Moleküler Baskılama Temelli Kuvars Kristal Mikroterazi (QCM) Sensörler ile Klorpirifos Tayini

Öz

Pestisitler tarımsal üretimin kontrolü gibi pek çok alanda yaygın olarak kullanılmaktadır. Bu maddeler tarımsal ürünlerin artmasına katkıda bulunur ancak toksik özelliklerinden dolayı canlılar üzerinde olumsuz etkilere sebep olmaktadır. Bu nedenle pestisitlerin tayin edilmesi, hem insanlar üzerinde artan toksik etkilerinden hem de kullanımının kontrol altına alınmasından dolayı büyük bir öneme sahiptir. Bu çalışmada, çevre sularında oldukça seçici, hassas, hızlı ve gerçek zamanlı bir ölçüm ile klorpirifos tayini için moleküler baskılama tekniği kullanılarak yeni bir kuvars kristal mikroterazi (QCM) sensör hazırlanmış olup N-metakriloil-(L)-triptofan metil ester (MATrp) uygun fonksiyonel monomer olarak seçilmiştir. Daha sonraki aşamada bu monomer kompleksi etilen glikol dimetakrilat (EGDMA) ile polimerleştirilmiştir. Polimerleştirilmiş klorpirifos baskılı nanofilmler QCM sensör çiplerinin altın yüzeylerine bağlanarak temas açısı ölçümleri ve atomik kuvvet mikroskobu (AFM) ile karakterizasyonları yapılmıştır. Kalınlık ölçümleri ve AFM görüntüleri, nanofilmlerin homojen ve tek tabakalı olduğunu göstermiştir. Baskılanmış nanofilmlerin baskılanmamış nanofilmlere oranla daha fazla hedef moleküle duyarlılık gösterdiği belirlenmiştir. Langmuir adsorpsiyon modeli, bu afinite sistemi için en uygun model olarak belirlenmiştir. Klorpirifos baskılanmış nanofilmlerin seçiciliğini göstermek için aynı derişimdeki klorpirifos, diazinon ve paration çözeltilerinin yarışmalı adsorpsiyonu araştırılmıştır. Sonuç olarak, QCM sensörlerin hızlı yanıt, kullanım kolaylığı, yüksek hassasiyet ve seçicilik ve gerçek zamanlı ölçüm alınabilme gibi özelliklerinden dolayı pestisit analizleri için alternatif bir yöntem olarak kullanılma potansiyeline sahip olduğu belirlenmiştir.

Anahtar Kelimeler

• Klorpirifos,kuvars kristal mikroterazi (QCM),nanofilm,moleküler baskılanmış polimerler,pestisit

Chlorpyrifos Detection with Molecular Imprinted Based Quartz Crystal Microbalance (QCM) Sensors

Öz

Pesticides have been widely used in many fields such as agricultural products control. Although these substances contribute to increasing of agricultural products, they have also many negative effects on the health due to contamination. Thus, it appears that pesticides have great effects on the management and increase the toxicity effects on humans. In this study, we were prepared to a new quartz crystal microbalance (QCM) sensor by using molecular imprinting technique for highly sensitive and selective, fast and realtime detection of chlorpyrifos in environmental waters. N-methacryloyl-(L)-tryptophan methyl ester (MATrp) was selected as a proper functional monomer and this monomer complex was polymerized with ethylene glycol dimethacrylate (EGDMA). Then, chlorpyrifos imprinted nanofilms were characterized by atomic force microscopy (AFM) and contact angle measurements binding to the gold surfaces of the QCM sensor chips. The thickness measurements and AFM observations indicated that the nanofilms were almost homogeneous and monolayer. The imprinted nanofilms were found to show more sensitivity towards the target molecule than the non-imprinted nanofilms. Langmuir adsorption model was determined as the most suitable model for this affinity system. In order to show the selectivity of the chlorpyrifos imprinted nanofilms, competitive adsorption of chlorpyrifos, diazinon and parathion solutions at the same concentration was investigated. As a result of, QCM sensors determined to have a potential to be used as an alternative method for pesticides analysis due to such properties fast response, easy-to-use properties, precision, selective and real-time measurement capability.

Anahtar Kelimeler

• Chlorpyrifos,nanofilm,pesticide,molecular imprinted polymer,quartz crystal microbalance (QCM)

Kaynakça

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