Research Article
Year 2019,
, 1316 - 1326, 01.09.2019
Abstract
References
- Apak R, Gorinstein S, Bohm V, Schaich KM, Özyürek M, et al. 2013. Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report). Pure and Applied Chemistry, 85: 957-998.
- Arnao MB, 2000. Some methodological problems in the determination of antioxidant activity using chromogen radicals: a practice case. Tr Food Sc Technol, 11(11): 419-421.
- Ascione F, Vasaturo A, Caserta S, D’Esposito V, Formisano P, Guido S, 2016. Comparison between fibroblast wound healing and cell random migration assays in vitro. Experimental Cell Research, 347: 123-132.
- Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M, 2008. Growth factors and cytokines in wound healing. Wound Repair Regen., 16: 585-601.
- Brand-Williams W, Cuvelier ME, Berset C, 1995. Use of free radical method to evaluate antioxidant activity. Food Science and Technology, 28: 25-30.
- Braun-falco O, Plewig G, Wolff HH, Burgdorf WHC (Eds.). 2000. Dermatology. Second ed. Springer, Berlin/Heidelberg/Newyork.
- Cornejo A, Jiménez JM, Caballero L, Melo F, Maccioni RB, 2011. Fulvic acid inhibits aggregation and promotes disassembly of tau fibrils associated with Alzheimer’s disease. J Alzheimers Dis., 27: 143-153.
- De Donatis A, Cirri P, 2009. Understanding the specificity of receptor tyrosine kinases signaling. Commun Integr Biol., 1: 156-157.
- Ebeling S, Naumann K, Pollok S et al. 2014. From a traditional medicinal plant to rational drug: Understanding the clinically proven wound healing efficacy of birch bark extract. PLoS One, 9: e86147.
- Fronza M, Heinzmann B, Hamburger M et al., 2009. Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol., 126: 463-467.
- Gao Y, Zhu J, Bao H, Hector V, Zhao B, Chu Z, 2018. Effect of lignite fulvic acid on growth, antioxidant ability, and HSP70 of Pacific white shrimp, Litopenaeus vannamei. Aquaculture Iternational, 26(6): 1519-1530. Guo S, Di Pietro LA, 2006. Factors Affecting Wound Healing. J Dent Res., 89: 219-29.
- Guzmán-Martinez L, Farías GA, Maccioni RB, 2013. Tau oligomers as potential targets for Alzheimer’s diagnosis and novel drugs. Frony Neurol., 4: 167.
- Hoang AM, Oates TW, Cochran DL, 2000. In vitro wound healing responses to enamel matrix derivative. Journal of Periodontology, 71(8): 1270-1277.
- Jurcsik I, 1994. Possibilities of applying humic acids in medicine, wound healing and cancer therapy. In: Senesi N, Milano TM., editors. Humic Substances in the Global Environment. Newyork: Elsevier; 1994. pp. 1331–1336.
- Liang CC, Park AY, Guan JL, 2007. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2): 329-333.
- MacCarthy P, 2001. The principles of humic substances. Soil Sci., 166: 738-751.
- Martin P, 1997. Wound healing–aiming for perfect skin regeneration. Science, 276: 75–81.
- Mossman T, 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65: 55-63.
- Motojima HO, Villareal M, Han J, Isoda H, 2011. Microarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid. Cytotechnology, 63: 181-190.
- Rasik AM, Shukla A, 2000. Antioxidant status in delayed healing type of wounds. Int. J Exp Pathol., 81: 257-263.
- Ruch RJ, Cheng SJ, Klaunig JE, 1989. Prevention of cyto-toxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 10(6): 1003-1008.
- Schafer M, Werner S, 2008. Oxidative stres in normal and impaired wound repair. Pharmacol Res., 58: 165-171.
- Sherry L, Millhouse E, Lappin DF, Murray C, Culshaw S, Nile CJ,Ramage G, 2013. Investigating the biological properties of carbohydrate derived fulvic acid (CHD-FA) as a potential nove therapy fort he management of oral biofilm infections. BMC Oral Health, 13: 47.
- Smith MB, March J, 2001. March’s Advanced Organic Chemistry, Reactions, Mechanisms, and Structure, Wiley, 484.
- Sönmez YM, 2011 Fulvik Asit Prosesinin Geliştirilmesi ve Tasarımı (Development and Design of Fulvic Acid Process). M.Sc. Thesis, Gazi University, Institute of Science and Technology, Ankara, Turkey.
- Tachibana Y, Hiribe S, Tawa R, 2004. Studies of antioxidative activity of humic substances in peat (1). Trace Nutrients Res., 23: 104-108.
- Tominaga H, Ishiyama M, Ohseto F, Sasamoto K, Hamamoto T, Suzuki K, Watanabe M, 1999. A water-soluble tetrazolium salt useful for colorimetric cell viability assay. Analytical Communications, 36: 47-50.
- Ueda J, Ikota N, Shinozuka T, Yamaguchi T, 2004. Reactive oxygen species scavenging ability of a new compound derived from weathered coal. Specrochimica Acta Part A, 60: 2487-2492.
- Ullah A, Khan A, Khan I, 2016. Diabetes mellitus and oxidative stres-A concise review. Saudi Pharmaceutical Journal, 24: 547-553.
- Van Rensburg CEJ, Malfeld SCK, Dekker J, 2001. Topical application of oxifulvic acid suppressess the cutaneous immune response in mice. Drug Dev Res., 53: 29-32.
- Winkler J, Ghosh S, 2018. Therapeutic potential of fulvic acid in chronic inflammatory diseases and diabetes. Journal of Diabetes Research, https://doi.org/10.1155/2018/5391014
- Zouboulis CC, 2000. Human skin: an independent peripheral endocrine organ. Hormone Research in Paediatrics, 54: 230-242.
Year 2019,
, 1316 - 1326, 01.09.2019
Abstract
This study aimed to investigate the antioxidant and in vitro wound healing activities of fulvic acid (FA) purified from Hüsamlar leonardite in Muğla/Turkey. Purified FA was analyzed with FT/IR spectrophotometer and flame photometer for determining functional groups and cation impurities, respectively. 1, 5, 10, 20, 30, 50, 100 μg mL-1 concentrations of FA were tested for antioxidant activity (by using DPPH radical scavenging and H2O2 scavenging assays). Also, effect of FA on BJ human foreskin fibroblast and HaCaT spontaneously immortalized non-tumorigenic human keratinocyte cells proliferation was tested by in vitro MTT and WST-8 assays and on cell migration by wound healing assay (scratch assay). The results of the study show that the FA has a low DPPH radical scavenging activity, but it exhibited high H2O2 scavenging activity at low concentrations. The effect of FA on the proliferation of BJ and HaCaT cells varied according to the cell type, FA concentration and treatment time. However, it accelerated wound healing by increasing cell migration, especially in HaCaT cells. 1 and 10 μg mL-1 FA had a significant wound healing effect on BJ cells, and all concentrations of FA had a significant wound healing effect on HaCaT cells at the end of the 24 h treatments.
References
- Apak R, Gorinstein S, Bohm V, Schaich KM, Özyürek M, et al. 2013. Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report). Pure and Applied Chemistry, 85: 957-998.
- Arnao MB, 2000. Some methodological problems in the determination of antioxidant activity using chromogen radicals: a practice case. Tr Food Sc Technol, 11(11): 419-421.
- Ascione F, Vasaturo A, Caserta S, D’Esposito V, Formisano P, Guido S, 2016. Comparison between fibroblast wound healing and cell random migration assays in vitro. Experimental Cell Research, 347: 123-132.
- Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M, 2008. Growth factors and cytokines in wound healing. Wound Repair Regen., 16: 585-601.
- Brand-Williams W, Cuvelier ME, Berset C, 1995. Use of free radical method to evaluate antioxidant activity. Food Science and Technology, 28: 25-30.
- Braun-falco O, Plewig G, Wolff HH, Burgdorf WHC (Eds.). 2000. Dermatology. Second ed. Springer, Berlin/Heidelberg/Newyork.
- Cornejo A, Jiménez JM, Caballero L, Melo F, Maccioni RB, 2011. Fulvic acid inhibits aggregation and promotes disassembly of tau fibrils associated with Alzheimer’s disease. J Alzheimers Dis., 27: 143-153.
- De Donatis A, Cirri P, 2009. Understanding the specificity of receptor tyrosine kinases signaling. Commun Integr Biol., 1: 156-157.
- Ebeling S, Naumann K, Pollok S et al. 2014. From a traditional medicinal plant to rational drug: Understanding the clinically proven wound healing efficacy of birch bark extract. PLoS One, 9: e86147.
- Fronza M, Heinzmann B, Hamburger M et al., 2009. Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol., 126: 463-467.
- Gao Y, Zhu J, Bao H, Hector V, Zhao B, Chu Z, 2018. Effect of lignite fulvic acid on growth, antioxidant ability, and HSP70 of Pacific white shrimp, Litopenaeus vannamei. Aquaculture Iternational, 26(6): 1519-1530. Guo S, Di Pietro LA, 2006. Factors Affecting Wound Healing. J Dent Res., 89: 219-29.
- Guzmán-Martinez L, Farías GA, Maccioni RB, 2013. Tau oligomers as potential targets for Alzheimer’s diagnosis and novel drugs. Frony Neurol., 4: 167.
- Hoang AM, Oates TW, Cochran DL, 2000. In vitro wound healing responses to enamel matrix derivative. Journal of Periodontology, 71(8): 1270-1277.
- Jurcsik I, 1994. Possibilities of applying humic acids in medicine, wound healing and cancer therapy. In: Senesi N, Milano TM., editors. Humic Substances in the Global Environment. Newyork: Elsevier; 1994. pp. 1331–1336.
- Liang CC, Park AY, Guan JL, 2007. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2): 329-333.
- MacCarthy P, 2001. The principles of humic substances. Soil Sci., 166: 738-751.
- Martin P, 1997. Wound healing–aiming for perfect skin regeneration. Science, 276: 75–81.
- Mossman T, 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65: 55-63.
- Motojima HO, Villareal M, Han J, Isoda H, 2011. Microarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid. Cytotechnology, 63: 181-190.
- Rasik AM, Shukla A, 2000. Antioxidant status in delayed healing type of wounds. Int. J Exp Pathol., 81: 257-263.
- Ruch RJ, Cheng SJ, Klaunig JE, 1989. Prevention of cyto-toxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 10(6): 1003-1008.
- Schafer M, Werner S, 2008. Oxidative stres in normal and impaired wound repair. Pharmacol Res., 58: 165-171.
- Sherry L, Millhouse E, Lappin DF, Murray C, Culshaw S, Nile CJ,Ramage G, 2013. Investigating the biological properties of carbohydrate derived fulvic acid (CHD-FA) as a potential nove therapy fort he management of oral biofilm infections. BMC Oral Health, 13: 47.
- Smith MB, March J, 2001. March’s Advanced Organic Chemistry, Reactions, Mechanisms, and Structure, Wiley, 484.
- Sönmez YM, 2011 Fulvik Asit Prosesinin Geliştirilmesi ve Tasarımı (Development and Design of Fulvic Acid Process). M.Sc. Thesis, Gazi University, Institute of Science and Technology, Ankara, Turkey.
- Tachibana Y, Hiribe S, Tawa R, 2004. Studies of antioxidative activity of humic substances in peat (1). Trace Nutrients Res., 23: 104-108.
- Tominaga H, Ishiyama M, Ohseto F, Sasamoto K, Hamamoto T, Suzuki K, Watanabe M, 1999. A water-soluble tetrazolium salt useful for colorimetric cell viability assay. Analytical Communications, 36: 47-50.
- Ueda J, Ikota N, Shinozuka T, Yamaguchi T, 2004. Reactive oxygen species scavenging ability of a new compound derived from weathered coal. Specrochimica Acta Part A, 60: 2487-2492.
- Ullah A, Khan A, Khan I, 2016. Diabetes mellitus and oxidative stres-A concise review. Saudi Pharmaceutical Journal, 24: 547-553.
- Van Rensburg CEJ, Malfeld SCK, Dekker J, 2001. Topical application of oxifulvic acid suppressess the cutaneous immune response in mice. Drug Dev Res., 53: 29-32.
- Winkler J, Ghosh S, 2018. Therapeutic potential of fulvic acid in chronic inflammatory diseases and diabetes. Journal of Diabetes Research, https://doi.org/10.1155/2018/5391014
- Zouboulis CC, 2000. Human skin: an independent peripheral endocrine organ. Hormone Research in Paediatrics, 54: 230-242.
There are 32 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Structural Biology |
| Journal Section | Biyoloji / Biology |
| Authors | |
| Publication Date | September 1, 2019 |
| Submission Date | January 8, 2019 |
| Acceptance Date | June 14, 2019 |
| Published in Issue | Year 2019 |