Dactylorhiza umbrosa (Kar. & Kir.) Nevski (Orchidaceae)'nin Antioksidan Özellikleri, İz Element ve Mineral Kompozisyonunun Değerlendirilmesi

Öz

Bu çalışmada, Türkiye'nin Doğu Anadolu Bölgesi'nde yetişen Dactylorhiza umbrosa (Kar. & Kir.) Nevski (Orchidaceae)'in antioksidan özellikleri, iz elementleri ve mineral bileşimleri belirlenmiştir. İz element ve mineral konsantrasyonları, indüktif eşleşmiş plazma-optik emisyon spektrometresi (ICP-OES) kullanılarak belirlenmiştir. Önemli eser elementler olan Mn, Cu, Co ve Se miktarlarının yüksek olduğu ve Mg mineralinin miktarının da oldukça yüksek olduğu tespit edilmiştir. Dactylorhiza umbrosa (Kar. & Kir.) Nevski bitkisinin element miktarlarının sırasıyla Sr> Mg> V> Mn> Li> Ti> Cu> Se> Cr> Pb> Co> As> Be> Zn> Cd olarak sıralandığı belirlenmiştir. Dactylorhiza umbrosa (Kar. & Kir.) Nevski'nin metanol ekstraktındaki toplam antioksidan aktivite, toplam fenolik ve flavonoid içerikleri sırasıyla 12.44 ± 0.20 mM askorbik asit g-1, 4.97 ± 0.48 mg gallik asit g-1 ve 4.51 ± 0.29 mg quarcetin g-1 olarak belirlendi. Metanol ekstraktında bazı eser elementlerin yüksek seviyelerinin bulunması ve antioksidan aktivite, toplam fenolik ve flavonoid içeriklerinin varlığı, gösterilen antioksidan özellikler açısından önemli olabilir.

Anahtar Kelimeler

• Dactylorhiza umbrosa,antioksidan,iz element

Evaluation of Antioxidant Properties, Trace Element and Mineral Composition of Dactylorhiza umbrosa (Kar. & Kir.) Nevski (Orchidaceae)

Öz

In this study, antioxidant properties, trace element and mineral composition of Dactylorhiza umbrosa (Kar. & Kir.) Nevski (Orchidaceae) grown in the East Anatolia Region of Turkey was determined. The concentrations of trace element and mineral were determined by using inductive paired plasma-optical emission spectrometer (ICP-OES). It has founded that the quantities of important trace elements Mn, Cu, Co and Se were high and also the quantity of the Mg mineral was quite high. Dactylorhiza umbrosa (Kar. & Kir.) Nevski plant's element quantities were determined to be Sr > Mg > V > Mn > Li > Ti > Cu > Se > Cr > Pb > Co > As > Be > Zn > Cd respectively. Total antioxidant activity, total phenolic and flavonoid contents were 12.44 ± 0.20 mM ascorbic acid g-1, 4.97 ± 0.48 mg gallic acid g-1 and 4.51 ± 0.29 mg quarcetin g-1 respectively in methanol extracts of Dactylorhiza umbrosa (Kar. & Kir.) Nevski plant. The presence of high levels of some trace elements, antioxidant activity, total phenolic and flavonoid contents in the methanol extract may have important to the showed antioxidant properties.

Anahtar Kelimeler

• Dactylorhiza umbrosa,antioxidant,trace element

Kaynakça

1. Agay D, Anderson RA, Sandre C, Bryden NA, Alonso A, Roussel AM, Chancerelle Y, 2005. Alterations of Antioxidant Trace Elements (Zn, Se, Cu) and Related Metallo-Enzymes in Plasma and Tissues Following Burn İnjury in Rats. Burns, 31: 366-371.
2. Chimsook T, 2016. Phytochemical Screening, Total Phenolic Content, Antioxidant Activities and Cytotoxicity of Dendrobium signatum Leaves. MATEC Web of Conferences, 62: 03005.
3. Esetlili BÇ, Pekcan T, Çobanoğlu Ö, Aydoğdu E, Turan S, Anaç D, 2014. Essential Plant Nutrients and Heavy Metals Concentrarions of Some Medicinal and Aromatic Plants. Journal of Agricultural Science, 20: 239-247.
4. Gamez-Meza N, Noriega-Rodriguez JA, Medina-Juarez LA, Ortega-Garcia J, Cazarez-Casanova R, Argulo-Guerreo O, 1999. Antioxidant Activity in Soybean oil of Extracts from Thompson Grape Bagasse. Journal of the American Oil Chemists Society, 76: 1445-47.
5. Ghorbani A, Gravendeel B, Naghibi F, Boer H, 2014. Wild Orchid Tuber Collection in Iran: A Wake-Up Call For Conservation. Biodiversity and Conservation, 23: 2749-2760.
6. Gulcin I, Sat IG, Beydemir S, Elmastas M, Kufrevioglu OI, 2004. Comparison of Antioxidant Activity of Clove (Eugenia caryophylata Thunb) Buds and Lavender (L stoechas L.). Food Chemistry, 8: 393.
7. Jan AT, Azam M, Siddiqui K, Ali A, Choi I, Hak QMR, 2015. Heavy Metals and Human Health: Mechanistic Insight into Toxicity and Counter Defence System of Antioxidants. International Journal of Molecular Sciences, 16: 29592-29630.
8. Kumar NJİ, Soni H, Kumar RN, 2007. Characterization of Heavy Metals in Vegetables Using Inductive Coupled Plasma Analyzer (ICPA). Journal of Applied Science and Environmental Management, (11)3: 75-79.

9. Lamasion J, Carnat A, Petitjean-Freytet C, 1990. Tannin Content and Inhibiting Activity of Elastase in Rosaceae. Annales Pharmaceutiques Françaises, 48: 335-340.
10. McLaughlin MJ, Parker DR, Clarke JM, 1999. Metals And Micronutrients – Food Safety Issues. Field Crops Research, 60: 143-163.
11. Minh TN, Khang DT, Tuyen PT, Minh LM, Anh LH, Quan NV, Ha PTT, Quan NT, Toan NP, Elzaawely AA, Xuan TD, 2016. Phenolic Compounds and Antioxidant Activity of Phalaenopsis Orchid Hybrids. Antioxidants, 5(3): 31.
12. Nguyen HC, Lin KH, Huang MY, Yang CM, Shih TH, Hsiung TC, Lin YC, Tsao FC, 2018. Antioxidant Activities of the Methanol Extracts of Various Parts of phalaenopsis Orchids with White, Yellow and Purple Flowers. Not Bot Horti Agrobo, 46(2): 457-465.
13. Nielsen FH, Jhonson LAK, 2017. Data from Controlled Metabolic Ward Studies Provide Guidance for the Determination of Status Indicators and Dietary Requirements for Magnesium. Biological Trace Elements Research, 177: 43-52.
14. Özcan M, 2004. Mineral Contents of Some Plants Used as Condiments in Turkey. Food Chemistry, 84: 437–440.
15. Özdemir F, Esen E, 2004. Stronsiyum ve Osteoporoz. Osteoporoz Dünyasından, 10: 77-79.
16. Prieto P, Pineda M, Aguilar M, 1999. Spectrophotometric Quantitation of Antioxidant Capacitiy Through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E. Analytical Biochemistry, 269: 337-341.
17. Rattana K, Sangchanjiradet S, 2017. Micropropagation of Dendrobium Signatum Rchb.F. Pertanika Journal of Tropical Agricultural Science, 40(4): 577 – 586.
18. Sandal G, 2009. Doğu Akdeniz Bölgesinde Yetişen Orkideler ve Yetişme Ortamı Nitelikleri ile Tehdit Faktörlerinin Araştırılması, Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi (Basılmış).
19. Shah AN, Tanveer M, Saddam H, Yang G, 2016. Beryllium in the Environment: Whether Fatal for Plant Growth? Reviews in Environmental Science and Bio/Technology, 15: 549-561.
20. Shapoo AG, Kaloo ZA, Ganie AH, Khuroo AA, Singh S, 2016. Dactylorhiza umbrosa (Kar. & Kir.) Nevski (Orchidaceae): An Addition to Flora of India from Kashmir Himalaya. Check List: The Journal of Biodiversity Data, 12: 3 1904.
21. Singh NKS, Devi CB, Singh TS, Singh NR, 2010. Trace Elements of some Selected Medicinal Plants of Manipur. Indian Journal of Natural Products and Resources, 2: 227-231.
22. Singleton VL, Orthofer R, Lamuela RRM, 1999. Analysis of Total Phenol and other Oxidation Substrates and Antioxidants by Means of Folin Ciocalteu Reagent. Methods in Enzymology, 299: 152-178.
23. Štajner D, Popovic´ BM, Kapor A, Boza P, Štajner M, 2010. Antioxidant and Scavenging Capacity of Anacamp. pyrimidalis L.–Pyrimidal Orchid from Vojvodina. Phytotherapy Research, 24:759–763.
24. Tudosie MS, Păun SC, Ionică M, Ardelean L, Bumbea V, Ciupan R, Mureşan A, Mladin C, 2012. Study of Lithium Elimination in Patients with Chronic Renal Disease. Therapeutics, Pharmacology and Clinical Toxicology, 2012: 202-209.
25. Urgeova E, Polivka L, 2009. Secondary Metabolites with Antibacterial Effects from Leaves of Different Hop Cultivars During Vegetal Periods. Nova Biotechnologica, 9: 327-332.
26. Vukivećević T, 2012. Toxic Effects of Cadmium. Acta Medica Medianae, 51: 65-70.
27. Wani AL, Ara A, Usmani JA, 2015. Lead Toxicity: A Review. Interdisciplinary Toxicology, 8: 55-64.
28. Yi OS, Meyer A, Frankel N, 1997. Antioxidant Activity of Grape Extracts in a Lecithin Liposome System. Journal of the American Oil Chemists Society, 74: 1301-1307.
29. Zurera G, Estrada B, Rineon F, Pozo R, 1987. Lead and Cadmium Contamination Levels in Ediple Vegatables. Bulltein of Environmental Contamination and Toxicology, 38: 805-812.