Synthesis, characterization, and inhibitory properties of novel N-benzylbenzimidazole-silver(I) complexes on carbonic anhydrase and polyphenol oxidase enzymes

Year 2020, , 320 - 327, 01.03.2020

Adem Ergün , Mert Olgun Karataş

https://doi.org/10.21597/jist.551498

Cited By: 1

Abstract

The
biological properties of silver complexes are well known but the studies about
the enzyme inhibitory properties of them are very rare. On the other hand, it
is known that one of the anticancer mechanisms of action of benzimidazole
derivatives is enzyme inhibition. Therefore, in this study, five novel N-benzylbenzimidazole-silver(I)
complexes were synthesized and fully characterized by 1H NMR, 13C
NMR, IR, mass spectroscopic methods and elemental analyses. The inhibitory
properties of the synthesized complexes were tested on the activity of human
carbonic anhydrase I - II and polyphenol oxidase, and it was observed that the
complexes inhibited the activity of these enzymes with the IC50
values of 29.58-32.65 µM, 21.05-23.65 µM and 30.38-44.45 µM, respectively. We
suggest that by also considering the low toxicity of silver compounds to human
body at low concentrations, the evaluated complexes in this study are promising
agents in the treatment of glaucoma and hyperpigmentation as carbonic anhydrase
and polyphenol oxidase inhibitors.

Keywords

benzimidazole, silver, carbonic anhydrase, polyphenol oxidase

Yeni N-benzilbenzimidazol-gümüş(I) komplekslerinin sentezi, karakterizasyonu, karbonik anhidraz ve polifenol oksidaz enzimleri üzerindeki inhibitör özellikleri

Abstract

Gümüş
komplekslerinin biyolojik özellikleri çok iyi bilinmesine rağmen enzim
inhibisyonu özelliklerine yönelik çalışmalar oldukça sınırlıdır. Diğer taraftan
benzimidazol türevlerinin anti-kanser etki mekanizmalarından birinin enzim
inhibisyonu olduğu bilinmektedir. Bu nedenle bu çalışmada, beş yeni N-benzilbenzimidazol-gümüş(I) kompleksi
sentezlendi, 1H NMR, 13C NMR, IR ve kütle spektroskopik
yöntemleri ve elementel analiz sonuçları ile tamamen karakterize edildi. Elde
edilen komplekslerin insan karbonik anhidraz I - II ve polifenol oksidaz
enzimleri üzerindeki inhibisyon etkileri incelendi ve komplekslerin bu
enzimlerin aktivitelerini sırasıyla 29.58-32.65 µM, 21.05-23.65 µM ve
30.38-44.45 µM’lık IC50 değerleri ile inhibe ettiği gözlendi. Gümüş
bileşiklerinin insan vücudunda düşük konsantrasyonlarda toksik etki göstermediği
de göz önüne alınırsa, bu çalışmada test edilen kompleksler karbonik anhidraz
inhibitörü olarak glokom tedavisinde ve polifenol oksidaz inhibitörü olarak ise
hiper-pigmentasyon hastalığı tedavisinde kullanılmak için umut verici
adaylardır.      

Keywords

Benzimidazol, gümüş, karbonik anhidraz, polifenol oksidaz

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