Antibacterial Activity and Biofilm Property of Silver Nanoparticles Synthesized by Using Saintpaulia Aqueous Leaf Extract

Öz

Today, nanoparticles are effectively used in different areas. Initially, physical and chemical methods were used in the synthesis of nanoparticles. Biosynthesis (green synthesis) has emerged as an alternative to overcome the toxic effect of chemically synthesized nanoparticles. In this study, green synthesis of silver nanoparticles (AgNPs) with the leaf extract of African violet plant (Saintpaulia) was carried out. As a result of the characterization, it was determined that nanoparticles were formed at a spherical size of 40.4 nm with a spectrum showing a spectrum at a wavelength of 432 nm. Antibacterial and biofilm inhibition activities of AgNPs against four Gram-positive and four Gramnegative were determined with bacterial strains. Silver nanoparticles at 10 mM concentration showed bacteriocidal activity against all bacterial strains. In the antibiotic activity study, the highest inhibition percentage was obtained against the Salmonella infantis strain at 80.3% at a concentration of 10 mM.

Anahtar Kelimeler

• Saintpaulia,Silver nanoparticles,Green synthesis,Antibacterial activity,Antibiofilm activity

Saintpaulia Sulu Yaprak Özütü Kullanılarak Sentezlenen Gümüş Nanopartiküllerin Antibakteriyel ve Antibiyofilm Aktivitesi

Öz

Günümüzde nanopartiküller farklı alanlarda etkin bir şekilde kullanılmaktadır. İlk zamanlar nanopartiküllerin sentezinde fiziksel ve kimyasal yöntemler kullanılmaktaydı. Kimyasal olarak sentezlenmiş nanoparçacıkların toksik etkisinin üstesinden gelmek için biyosentez (yeşil sentez) bir alternatif olarak ortaya çıkmıştır. Bu çalışmada, Afrika menekşesinin (Saintpaulia) yaprak özütü ile gümüş nanopartiküllerin (AgNP’lerin) yeşil sentezi gerçekleştirilerek karakterizasyonu yapılmıştır. Karakterizasyon sonucunda ~432 nm dalga boyunda spektrum gösteren, 40.4 nm büyüklüğünde küresel boyutta nanopartiküllerin oluştuğu belirlenmiştir. AgNP’lerin dört Gram-pozitif ve dört Gram-negatif bakteri suşlarına karşı antibakteriyel ve biyofilm inhibisyon aktiviteleri belirlenmiştir. 10 mM konsantrasyonda gümüş nanopartiküller tüm bakteri suşlarına karşı bakteriyosidal etki göstermiştir. Antibiyofilm aktivitesi çalışmasında ise en yüksek inhibisyon yüzdesi 10 mM konsantrasyonda %80.3 oranında Salmonella infantis suşuna karşı elde edilmiştir.

Anahtar Kelimeler

• Saintpaulia,Gümüş nanopartiküller,Yeşil sentez,Antibakteriyel aktivite,Antibiyofilm aktivite

Destekleyen Kurum

Bartın Üniversitesi

Kaynakça

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