Evaluation of the Effects of Some Antibiotics on Biofilm Forming Staphylococci Isolates

Öz

Staphylococci are pathogenic microorganisms that have various virulence factors. One of the most important of these virulence factors is biofilm production. Some staphylococci become resistant to antibiotics by producing biofilms and treatment of infections becomes more difficult. The aim of this study was to determine the effect of vancomycin, erythromycin, oxacillin antibiotics on planktonic and biofilm forming forms of staphylococci isolates which were isolated from mastitis cow's milk in a previous study. Minimum inhibitory concentration (MIC), minimum biofilm inhibitory concentration (MBIC), minimum biofilm eradication concentration (MBEC) values of antibiotics were determined. When the MIC, MBIC and MBEC values of antibiotics were compared; the highest range of MIC values was determined for erythromycin (32-64 μg ml-1). MBIC and MBEC values were similar for all test antibiotics. As a result of this study, staphylococci isolates were found to be more resistant to antibiotics by producing biofilm and were affected by higher doses of antibiotics than their free-living forms.

Anahtar Kelimeler

• Antibiotic resistance,biofilm,staphylococci

Bazı Antibiyotiklerin Biyofilm Oluşturan Stafilokok İzolatları Üzerindeki Etkilerinin Değerlendirilmesi

Öz

Stafilokoklar çeşitli virülans faktörlere sahip olan patojen mikroorganizmalardır. Bu virülans faktörlerden birisi biyofilm üretimidir. Bazı stafilokoklar, biyofilm üreterek antibiyotiklere karşı dirençli hale gelmektedir ve enfeksiyonların tedavisi zorlaşmaktadır. Bu çalışmanın amacı, daha önce bir çalışmada mastitisli inek sütlerinden izole edilmiş olan stafilokok izolatlarının planktonik ve biyofilm oluşturan formlarının üzerine vankomisin, eritromisin, oksasilin antibiyotiklerinin etkisini belirlemektir. Antibiyotiklerin minimum inhibitör konsantrasyonu (MİK), biyofilm minimum inhibitör konsantrasyonu (MBİK) ve minimum biyofilm eradikasyon konsantrasyonu (MBEK) değerleri belirlenmiştir. Antibiyotiklerin MİK, MBİK ve MBEK değerleri karşılaştırıldığında; en yüksek MİK değeri (32-64 μg ml-1) eritromisin için olduğu belirlenmiştir. MBİK ve MBEK değerleri ise tüm antibiyotikler için benzerlik göstermiştir. Bu çalışmanın sonucunda, stafilokok izolatlarının biyofilm oluşturarak antibiyotiklere karşı daha dirençli hale geldikleri ve serbest yaşayan formlarına göre daha yüksek dozda antibiyotiklerden etkilendikleri saptanmıştır.

Anahtar Kelimeler

• Antibiyotik direnci,biyofilm,stafilokok

Kaynakça

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