Kanserli ve Sağlıklı Larenks Dokularında ICP-MS Yöntemi ile Eser Elementler Analizi

Yıl 2020, , 1779 - 1785, 01.09.2020

Ömer Faruk Koçak , Mevlüt Albayrak , Fatma Betül Özgeriş

https://doi.org/10.21597/jist.681303

Öz

Eser elementler vücudun düzgün çalışması için gereklidir. Biyolojik element konsantrasyonundaki değişiklikler çok sayıda patolojik durumda gözlenmiştir. Bu çalışmanın amacı, larenks dokularındaki eser element konsantrasyonunun değerlendirilmesidir. Çalışmada larenks kanseri teşhisi konmuş 9 hastanın kanserli larenks dokuları ve aynı hastaların sağlıklı larekns dokuları kullanılmıştır. Larenks dokularındaki Al, Cr, Mn, Co, Ni, Cu, Zn, As, Se, Ag, Cd, Sb ve Pb seviyeleri, Endüktif Eşleşmiş Plazma Kütle Spektrometresi (ICP-MS) kullanılarak belirlenmiştir. Sonuçlar kontrol grubu ile karşılaştırıldı ve larenks kanserli dokularda Cu seviyesinin sağlıklı dokulara göre anlamlı derecede (p <0.05) yüksek olduğu ve Al, Cr, Mn, As, Se, Ag ve Sb elementlerinin derişiminin ise sağlıklı dokulara göre anlamlı derecede düşük olduğu bulundu. Bu sonuçlar, larenks kanseri etyolojisinde eser elementlerin önemli rolleri olduğunu düşündürmektedir.

Anahtar Kelimeler

Larenks kanseri, eser elementler, indüktif eşleşmiş plazma - kütle spektrometresi, FFPE doku

Teşekkür

Atatürk Üniversitesi Tıp Fakültesi Tıbbi Patoloji Anabilim Dalı'na FFPE doku örneklerini sağladığı için içten teşekkürlerimizi sunarız.

Trace Elements Analysis in Cancer and Healthy Larynx Tissues by ICP-MS Method

Öz

Trace elements are necessary for the body to function properly. Changes in biological element concentration have been observed in a number of pathological conditions. The aim of this study is to evaluate the trace element concentration in the larynx tissues. Cancer laryngeal tissues and healthy larynx tissues of the same patients were used in the study. Al, Cr, Mn, Co, Ni, Cu, Zn, As, Se, Ag, Cd, Sb and Pb levels in the larynx tissues were determined using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The results were compared with the control group and it was found that Cu level in tissues with laryngeal cancer was significantly higher (p <0.05) compared to healthy tissues, and the concentration of Al, Cr, Mn, As, Se, Ag, and Sb elements was significantly lower than healthy tissues. These results suggest that trace elements play an important role in the etiology of laryngeal cancer.

Anahtar Kelimeler

Laryngeal cancer, TRACE ELEMENTS, inductively coupled plasma - mass spectrometer, FFPE tissue

Kaynakça

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