Polymerization of Hydroquinone Using Horseradish Peroxidase Nanobiocatalyst

Öz

In this study, the effects of flower shaped hybrid nanobiocatalyst (HRP-Fe2+) containing horseradish peroxidase (HRP) enzyme and Fe2+ ions on the polymerization of hydroquinone were investigated. According to the obtained results, HRP-Fe2+ hybrid nanobiocatalyst in the presence of hydrogen peroxide (H2O2) initiator has shown enhanced catalytic stability at high reaction temperatures compared to that of free HRP enzyme. Poly(hydroquinone) was successfully synthesized with 44% of yield in pH 7.4 buffer at 60 oC reaction temperature with 5 weight % HRP-Fe2+catalyst loading. HRP-Fe2+ nanobiocatalyst also showed some degree of catalytic activity even at 70 oC reaction temperature without having denaturation, in which free HRP enzyme denatures. On the other hand, HRP-Fe2+ also showed lower catalytic activity in the polymerization of hydroquinone compared to that of the free HRP enzyme. It was observed that both polymerizations resulted in polymer product with almost the same yields, but the polymers obtained from using the free HRP enzyme had higher molecular weights in contrast with the polymers obtained from HRP-Fe2+ biocatalyst. It was concluded that the catalytic activity of the HRP-Fe2+ nanobiocatalyst slightly decreased compared to the free HRP enzyme.

Anahtar Kelimeler

• Horseradish peroxidase
• nanobiocatalyst
• hydroquinone
• enzymatic polymerization

Horseradish Peroksidaz Nano Biyokatalizörü İle Hidrokinon’un Polimerleştirilmesi

Öz

Bu araştırmada, Fe2+ iyonlarının horseradish peroksidaz (HRP) enzimiyle oluşturduğu çiçek şekilli hibrit nano biyokatalizörün (HRP-Fe2+), hidrokinon’un polimerleşmesi üzerine etkisi incelenmiştir. Elde edilen sonuçlara göre; HRP-Fe2+ biyokatalizörü ve hidrojen peroksit (H2O2) başlatıcısı kullanılarak gerçekleştirilen polimerleşmeler, serbest HRP enziminin kullanıldığı koşullara kıyasla yüksek sıcaklıklarda oldukça yüksek katalitik kararlılık göstermiştir. Poly(hidrokinon), pH 7.4 tamponu içerisinde 60 oC sıcaklıkta ve ağırlıkça %5 oranında HRP-Fe2+ biyokatalizörü eklenmesiyle %44 verimle sentezlenmiştir. HRP-Fe2+ biyokatalizörü, serbest HRP enziminin denatüre olduğu 70 oC gibi yüksek sıcaklıklarda bile bozunmaya uğramadan katalitik aktivite göstermiştir. HRP-Fe2+ biyokatalizörünün ayrıca serbest HRP enzimine kıyasla az da olsa daha düşük katalitik aktivite gösterdiği gözlenmiştir. Bu iki katalizörle gerçekleştirilen polimerleşmelerde verimlerin birbirine yakın olduğu, ancak serbest HRP enzimi kullanılarak elde edilen polimerlerin HRP-Fe2+ kullanılarak elde edilen polimerlere kıyasla daha yüksek molekül ağırlığına sahip olduğu gözlenmiştir. Buradan da HRP-Fe2+ biyokatalizörünün katalitik aktivitesinin, serbest HRP enzimine kıyasla azaldığı sonucuna varılmıştır.

Anahtar Kelimeler

• Horseradish peroksidaz
• nano biyokatalizör
• hidrokinon
• enzimatik polimerleşme

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