Characterization of Some Registered Chickpea (Cicer arietinum L.) Varieties with Simple Sequence Repetitions (SSRs) Markers

Yıl 2021, , 732 - 742, 01.03.2021

Şeyda Turkay Melike Bakır

https://doi.org/10.21597/jist.774375

Öz

Chickpea (Cicer arietinum L.) is an economically important edible legume plant that is grown worldwide. In this study, genetic similarities of 10 registered chickpea varieties which are registered after 2011 were investigated by using 6 polymorphic SSR markers selected from 15 SSR markers. The total number of alleles were 29 and the average number of alleles for each locus were 4.83. The average heterozygous rate was 0.62, PIC (polymorphism information content) was between 0.41 and 0.74 and an average of 0.58. It was observed that 10 chickpea varieties were divided into two groups with the genetic similarity dendogram created using Neighbor-joining and UPGMA from the genetic similarity matrix. In terms of genetic similarity, Sezenbey and Zuhal varieties (96%) registered by the Black Sea Agricultural Research Institute (96%) and Ilgaz and Aslanbey varieties (96%) registered by the Eastern Mediterranean Agricultural Research Institute were found the closest varieties. On the other hand, Seçkin registered by the Mediterranean Agricultural Research Institute and Akça registered by Transitional Zone Agricultural Research Institute were found the farthest varieties (21%). With this study, determining of the genetic similarities some registered chickpea varieties was aimed to contribute to chickpea breeding studies and the other genetic-based studies.

Anahtar Kelimeler

SSRs, Chickpea, genetic diversity, characterization

Bazı Tescilli Nohut (Cicer arietinum L.) Çeşitlerinin Simple Sequence Repeats (SSRs) Markörler ile Karakterizasyonu

Öz

Nohut (Cicer arietinum L.) dünya çapında yetiştirilen ve ekonomik açıdan önemli olan yemeklik baklagil bitkisidir. Bu çalışmada, 2011 yılı sonrası tescil edilen 10 tescilli nohut çeşidi 15 SSR markörü içerisinden polimorfizm gösteren 6 SSR markörü kullanılarak genetik benzerlikleri incelenmiştir. Toplam allel sayısı 29 ve her bir lokus için ortalama allel sayısı 4.83 olarak belirlenmiştir. Ortalama heterozigotluk oranı 0.62, PIC (polymorphism information content) değeri 0.41 ile 0.74 arasında ve ortalama 0.58 olarak bulunmuştur. Genetik benzerlik matriksinden Neighbour-joining ve UPGMA kullanılarak oluşturulan genetik ilişki dendogramı ile 10 nohut çeşidinin iki ayrı gruba ayrıldığı görülmüştür. Genetik benzerlik açısından birbirine en yakın çeşitlerin Karadeniz Tarımsal Araştırma Enstitüsü tarafından tescil edilen Sezenbey ve Zuhal çeşitleri (%96) ile Doğu Akdeniz Tarımsal Araştırma Enstitüsü tarafından tescil edilen Ilgaz ve Aslanbey çeşitleri (%96) olduğu, birbirlerine en uzak çeşitlerin ise Doğu Akdeniz Tarımsal Araştırma Enstitüsü tarafından tescil edilen Seçkin ile Geçit Kuşağı Tarımsal Araştırma Merkezi tarafından tescil edilen Akça çeşitleri (%21) olduğu tespit edilmiştir. Bu çalışma ile bazı tescilli nohut çeşitlerin genetik benzerlik ilişkisi belirlenmiş, nohut ıslah çalışmaları ve nohutta gerçekleştirilecek diğer genetik tabanlı çalışmalara katkıda bulunması amaçlanmıştır.

Anahtar Kelimeler

Nohut, SSRs, genetik çeşitlilik, karakterizasyon

Kaynakça

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