Acute Ecotoxicological and Histopathological Effects of Maxilon Blue 5G as an Azo Dye on Earthworms

Öz

Today, the effects of dyes on the environment and life health are important scientific issues. In this paper, for the first time, we report the histopathological and ecotoxicological studies of Maxilon blue 5G on earthworms as very important organisms for soil structure. Earthworms was exposed to Maxilon blue 5G by direct injection method with different doses in a range of 1.0-8000 mg L-1 for 48 h. The experimental analysis showed that some considerable morphological abnormalities in the earthworms were detected with the injection of 5000 mg L-1 and 8000 mg L-1 of Maxilon blue 5G dosages. LD50 values of Maxilon Blue 5G in earthworms’ experiments were calculated as 6324.56 mg L-1 after 48 h, and these values are the first experimental findings for the literature. The findings of the study were supported by histopathological investigations that are many severe tissue damages that were observed in the intestine and the whole body of earthworms injected with a high dosage of Maxilon blue 5G.

Anahtar Kelimeler

• Earthworm
• ecotoxicological
• dyestuff
• histopathological
• maxilon blue 5G

Bir Azo Boyası Olarak Maxilon Blue 5G'nin Solucanlar Üzerindeki Akut Ekotoksikolojik ve Histopatolojik Etkileri

Öz

Günümüzde boyaların çevre ve yaşam sağlığı üzerindeki etkileri önemli bilimsel konulardır. Maxilon blue 5G'nin toprak yapısı için çok önemli organizmalar olan solucanlar üzerindeki histopatolojik ve ekotoksikolojik çalışmalarını ilk kez bu yazıda sunuyoruz. Solucanlar, Maxilon blue 5G'ye, 1.0-8000 mg L-1 aralığında farklı dozlarda, direkt enjeksiyon yöntemiyle 48 saat süreyle maruz bırakıldı. Deneysel analiz, 5000 mg L-1 ve 8000 mg L-1 Maxilon blue 5G dozajlarının enjeksiyonu ile solucanlarda bazı önemli morfolojik anormalliklerin tespit edildiğini gösterdi. Maxilon Blue 5G'nin solucan deneylerindeki LD50 değerleri 48 saat sonra 6324.56 mg L-1 olarak hesaplanmıştır ve bu değerler literatür için ilk deneysel bulgulardır. Çalışmanın bulguları, yüksek dozda Maxilon blue 5G enjekte edilen solucanların bağırsaklarında ve tüm vücudunda gözlenen birçok ciddi doku hasarının histopatolojik incelemeleri ile desteklendi.

Anahtar Kelimeler

• Solucan
• Ekotoksikolojik etkiler
• Boyar madde
• Histopatoloji
• Maxilon blue 5G

Kaynakça

1. Albadarin AB, Mangwandi C, 2015. Mechanisms of Alizarin Red S and Methylene blue biosorption onto olive stone by-product: Isotherm study in single and binary systems. Journal of environmental managementanage, 164: 86–93.
2. Alderete BL, da Silva J, Godoi R, da Silva FR., Taffarel SR, da Silva LP, Picada JN, 2021. Evaluation of toxicity and mutagenicity of a synthetic effluent containing azo dye after advanced oxidation process treatment. Chemosphere, 263: 128291.
3. Alkan M, Doğan M, Turhan Y, Demirbaş Ö, Turan P, 2008. Adsorption kinetics and mechanism of maxilon blue 5G dye on sepiolite from aqueous solutions. Chemical Engineering Journal, 139(2): 213–223.
4. Balapure K, Bhatt N, Madamwar D, 2015. Mineralization of reactive azo dyes present in simulated textile waste water using down flow microaerophilic fixed film bioreactor. Bioresource Technology, 175: 1–7.
5. Banerjee A, Biswas JK, Pant D, Sarkar B, Chaudhuri P, Rai M, Meers E, 2019. Enteric bacteria from the earthworm (Metaphire posthuma) promote plant growth and remediate toxic trace elements. Journal of Environmental Management, 250: 109530.
6. Beer F, Urbat F, Franz CMAP, Huch M, Kulling SE, Bunzel M, Bunzel D, 2019. The human fecal microbiota metabolizes foodborne heterocyclic aromatic amines by reuterin conjugation and further transformations. Molecular Nutrition & Food Research, 63(10): 1801177.
7. Cai X, Yuan Y, Liao Z, Xing K, Zhu C, Xu Y, Yu L, Wang L, Wang S, Zhu X, Gao P, Zhang Y, Jiang Q, Xu P, Shu G, 2018. α-Ketoglutarate prevents skeletal muscle protein degradation and muscle atrophy through PHD3/ADRB2 pathway. The Faseb Journal, 32(1): 488–499.
8. Carmen Z, Daniel S, 2012. Textile organic dyes – characteristics, polluting effects and separation/elimination procedures from ındustrial effluents – a critical overview. Organic Pollutants Ten Years After the Stockholm Convention - Environmental and Analytical Update. InTech publications No:3, pp. 55-86, Shanghai-China.

9. Cerniglia CE, Freeman JP, Franklin W, Pack LD, 1982. Metabolism of azo dyes derived from benzidine, 3,3’-dimethylbenzidine and 3,3’ -dimethoxybenzidine to potentially carcinogenic aromatic amines by intestinal bacteria. Carcinogenesis, 3(11): 1255–1260.
10. Connon RE, Geist J, Werner I, 2012. Effect-based tools for monitoring and predicting the ecotoxicological effects of chemicals in the aquatic environment. Sensors, 12(9): 12741–12771.
11. Copaciu F, Opriş O, Coman V, Ristoiu D, Niinemets Ü, Copolovici L, 2013. Diffuse water pollution by anthraquinone and azo dyes in environment importantly alters foliage volatiles, carotenoids and physiology in wheat (Triticum aestivum). Water, Air, & Soil Pollution, 224: 1478.
12. De Oliveira GAR, Leme DM, de Lapuente J, Brito LB, Porredón C, Rodrigues L de B, Brull N, Serret JT, Borràs M, Disner GR, Cestari MM, De Oliveira DP, 2018. A test battery for assessing the ecotoxic effects of textile dyes. Chemico-Biological Interactions, 291: 171–179.
13. DeVito SC, 1993. Predicting azo dye toxicity. Critical Reviews in Environmental Science and Technology, 23(3): 249–324.
14. Eisenhauer N, 2010. The action of an animal ecosystem engineer: Identification of the main mechanisms of earthworm impacts on soil microarthropods. Pedobiologia, 53(6): 343–352.
15. Franco JH, da Silva BF, Dias EFG, de Castro AA, Ramalho TC, Zanoni MVB, 2018. Influence of auxochrome group in disperse dyes bearing azo groups as chromophore center in the biotransformation and molecular docking prediction by reductase enzyme: Implications and assessment for environmental toxicity of xenobiotics. Ecotoxicology and Environmental Safety, 160: 114–126.
16. Genázio Pereira PC, Reimão RV, Pavesi T, Saggioro EM, Moreira JC, Veríssimo Correia F, 2017. Lethal and sub-lethal evaluation of Indigo Carmine dye and byproducts after TiO2 photocatalysis in the immune system of Eisenia andrei earthworms. Ecotoxicology and Environmental Safety, 143: 275–282.
17. Gopinathan R, Kanhere J, Banerjee J, 2015. Effect of malachite green toxicity on non target soil organisms. Chemosphere, 120: 637–644.
18. Govindwar SP, Kurade MB, Tamboli DP, Kabra AN, Kim PJ, Waghmode TR, 2014. Decolorization and degradation of xenobiotic azo dye Reactive Yellow-84A and textile effluent by Galactomyces geotrichum. Chemosphere, 109: 234–238.
19. Haque MM, Haque MA, Mosharaf MK, Marcus PK, 2021. Decolorization, degradation and detoxification of carcinogenic sulfonated azo dye methyl orange by newly developed biofilm consortia. Saudi Journal of Biological Sciences, 28(1): 793-804.
20. Hassaan MA, El Nemr A, 2017. Health and environmental impacts of dyes: mini review. American Journal of Environmental Science and Engineering, 1: 64–67.
21. Kant R, 2012. Textile dyeing industry an environmental hazard. Natural Science, 04(1): 22–26.
22. Köktürk M, Altindağ F, Ozhan G, Çalimli MH, Nas MS, 2021. Textile dyes Maxilon blue 5G and Reactive blue 203 induce acute toxicity and DNA damage during embryonic development of Danio rerio. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 242:108947.
23. Langdon CJ, Piearce TG, Meharg AA, Semple KT, 2003. Interactions between earthworms and arsenic in the soil environment: a review. Environmental Pollution, 124(3): 361–373.
24. Lavelle P, Pashanasi B, Charpentier F, Gilot C, Rossi JP, Derouard L, André J, Ponge JF, Bernier N, 1998. Large-scale effects of earthworms on soil organic matter and nutrient dynamics. Lucie Press, pp.103–122.
25. Levine WG, 1991. Metabolism of AZO dyes: Implication for detoxication and activation. Drug Metabolism Reviews, 23(3&4): 253–309.
26. Moorthy AK, Rathi BG, Shukla SP, Kumar K, Bharti VS, 2021. Acute toxicity of textile dye Methylene blue on growth and metabolism of selected freshwater microalgae. Environmental Toxicology and Pharmacology, 82: 103552.
27. Nas MS, Kuyuldar E, Demirkan B, Calimli MH, Demirbaş O, Sen F, 2019. Magnetic nanocomposites decorated on multiwalled carbon nanotube for removal of Maxilon Blue 5G using the sono-Fenton method. Scientific Report, 9: 10850.
28. Nayak AK, Panda SS, Basu A, Dhal NK, 2018. Enhancement of toxic Cr (VI), Fe, and other heavy metals phytoremediation by the synergistic combination of native Bacillus cereus strain and Vetiveria zizanioides L. International Journal of Phytoremediation, 20(7): 682–691.
29. Park BS, Yoo JH, Kim JH, Kim JE, Lee SE, 2012. Biotransformation of endosulfan by the tiger worm, Eisenia fetida. Journal of Agricultural Chemistry and Environment, 1: 20-27.
30. Pino MR, Val J, Mainar AM, Zuriaga E, Español C, Langa E, 2015. Acute toxicological effects on the earthworm Eisenia fetida of 18 common pharmaceuticals in artificial soil. Science of The Total Environment, 518–519: 225–237.
31. Puvaneswari N, Muthukrishnan J, Gunasekaran P, 2006. Toxicity assessment and microbial degradation of azo dyes. Indian Journal of Experimental Biology, 44: 618-626.
32. Rawat D, Mishra V, Sharma RS, 2016. Detoxification of azo dyes in the context of environmental processes. Chemosphere, 155: 591–605.
33. Reile CG, Rodríguez MS, de Sousa Fernandes DD, de Araújo Gomes A, Diniz PHGD, Di Anibal CV, 2020. Qualitative and quantitative analysis based on digital images to determine the adulteration of ketchup samples with Sudan I dye. Food Chemistry, 328, 127101.
34. Sweeney EA, Chipman JK, Forsythe SJ, 1994. Evidence for Direct-Acting Oxidative Genotoxicity by Reduction Products of Azo Dyes. Environmental Health Perspectives, 102(6): 119-122.
35. Tkaczyk A, Mitrowska K, Posyniak A, 2020. Synthetic organic dyes as contaminants of the aquatic environment and their implications for ecosystems: a review. Science of The Total Environment, 717: 137222.
36. Washington TA, White JP, Davis JM, Wilson LB, Lowe LL, Sato S, Carson JA, 2011. Skeletal muscle mass recovery from atrophy in IL-6 knockout mice. Acta Physiologica, 202(4): 657–669.
37. Yang G, Chen C, Wang Y, Peng Q, Zhao H, Guo D, Wang Q, Qian Y, 2017. Mixture toxicity of four commonly used pesticides at different effect levels to the epigeic earthworm, Eisenia fetida. Ecotoxicology and Environmental Safety, 142: 29–39.
38. Yesudhason BV, Kanniah P, Subramanian ER, Pnesakki V, Rajendiran V, Sivasubramaniam S, 2018. Exploiting the unique phenotypes of the earthworm Eudrilus eugeniae to evaluate the toxicity of chemical substances. Environmental Monitoring and Assessment, 190: 145.
39. Zanoni TB, Lizier TM, Assis M das D, Zanoni MVB, De Oliveira DP, 2013. CYP-450 isoenzymes catalyze the generation of hazardous aromatic amines after reaction with the azo dye Sudan III. Food and Chemical Toxicology, 57: 217–226.
40. Zollinger H, 2003. Color Chemistry. Synthesis, Properties and Applications of Organic Dyes and Pigments. Verlag Helvetica Chimica Acta publications No:3, pp. 1–125, Zürich-Switzerland.