The Role of Curcumin-Loaded Solid Lipid Nanoparticles Labeled with Tc-99m in the Liver-Spleen Scintigraphy

Yıl 2016, Cilt: 6 Sayı: 3, 27 - 34, 30.09.2016

Hayrettin Eroğlu , Ayşe Yenilmez Arif Kursad Ayan Cemal Gündoğdu Sare Şipal

Öz

Recently, nanotechnology and molecular imaging felds are integrated to facilitate the diagnosis and
the treatment of diseases with create multi-functional nanoparticles. Nuclear medicine is one of the felds that beneft
from the multi-faceted and unique characteristics of new generation imaging agents that are made of nanoparticles.
This study investigated the feasibility of using curcumin-loaded SLNs in liver and spleen scintigraphy. Initially, the
curcumin-loaded solid lipid nanoparticles (C-SLN) were prepared using microemulsion and solidifcation at low
temperatures. The size of the C-SLN particle is measured by Laser diffraction (LD) method. Moreover, by applying
thermogravimetric analysis (TGA) it is observed that C-SLN particles are stable with temperature variation. Then,
they are radiolabeled with Technetium-99m (99mTc). Using the thin-layer chromatography method, the radiolabeling
effciency was found to be higher than 95%. 99mTc-C-SLNs were injected to the rabbit intravenously (i.v.) and
using a gamma camera, dynamic images were recorded. Liver and spleen were clearly displayed in these dynamic
images. In addition to scintigraphic fndings, the presence of 99mTc-C-SLNs in the liver and the spleen was observed
in the ex vivo biodistribution studies and the analyses performed under the fluorescence microscope.  

Anahtar Kelimeler

Biodistribution, curcumin, scintigraphy, solid lipid nanoparticle, Tc-99m

Tc-99m ile İşaretli Kurkumin Yüklü Katı Lipit Nanopartiküllerin Karaciğer-Dalak Sintigrafsindeki Rolü

Öz

Son zamanlarda, hastalıkların tanı ve tavisini kolaylaştırmak amacıyla, nanoteknoloji ve
moleküler görüntüleme alanları, çok fonksiyonlu nanopartiküllerin oluşturulması ile birleştirilmiştir.
Nanopartiküllerden oluşturulan yeni nesil görüntüleme ajanlarının sahip olduğu çok yönlü ve eşsiz
özelliklerinden faydalanan alanlardan biriside nükleer tıp alanıdır. Yapılan bu çalışmada; kurkumin
yüklü katı lipit nanopartiküllerin (K-KLN) karaciğer ve dalak sintigrafsinde kullanılabilirliliği
araştırıldı. Öncelikle, kurkumin yüklü katı lipit nanopartiküller (K-KLN) mikroemülsiyon ve düşüksıcaklıkda katılaştırma yöntemiyle hazırlandı. Lazer kırınımı (LD) analizi ile partikül boyutu belirlendi.
Termogravimetrik analizi (TGA) ile K-KLN’lerin sıcaklığa karşı stabil olduğu gözlemlendi. Daha sonra
K-KLN’ler Teknesyum-99m (99mTc) ile radyoaktif olarak işaretlendi. İnce katman kromatograf yöntemi
ile radyoaktif işaretleme etkinliği %95’den daha fazla olarak bulundu. 99mTc-K-KLN’ler intravenöz (i.v.)
olarak tavşana enjekte edildi ve gama kamera ile dinamik görüntüler kaydedildi. Elde edilen dinamik
görüntülerde karaciğer ve dalak belirgin bir şekilde görüntülendi. Sintigrafk bulgulara ek olarak
yapılan ex vivo biyodağılım çalışmaları ve floresan mikroskop çalışmalarında karaciğer ve dalakta
99mTc-K-KLN’lerin varlığı gözlemlendi.  

Anahtar Kelimeler

Biyodağılım, katı lipit nanopartikül, kurkumin, sintigraf, Tc-99m

Kaynakça

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