The Effects of Storage Time on Nutrient Composition and Silage Quality Parameters of Corn Silage Made in Plastic Mini Silo in Laboratory Conditions

Yıl 2016, Cilt: 6 Sayı: 3, 177 - 183, 30.09.2016

Betül Zera Sarıçiçek Birgül Yıldırım Zahide Kocabaş Emel Özgümüş Demir

Öz

This study was carried out to determine the effects of storage duration on nutrient composition and
silage quality parameters of corn silage made in plastic mini silo under laboratory conditions. Corn was used as a
silage material. Corn (31.87% of dry matter) was harvested at the dough stage, and ensiled at laboratory conditions
in PVC mini silos, for 90, 104, 118, 132, 146, 160, 174, 188 and 202 days. The dry matter, crude protein, ether
extract, ash and crude fber content of corn silage decreased with the longer storage time (P<0.05). However,
nitrogen free extract (NFE) content showed an increase througout the storage time. Storage time didn’t signifcantly
lead to change on EE content of corn silage. Lactic acid (LA) concentration of corn silage increased until the 104th
day, but it decreased between 104th and 188th days (P<0.05), after which it increased again. Prolongation of storage
time gave rise to fluctuation of LA concentration. Contrary to the decrease in LA concentration, acetic acid (AA)
concentration increased depending on storage time. Silage NH3-N concentration was highest at the 146th day, CO2
concentration increased until the 160th day, then, decreased in the other storage time (P<0.05). The lowest WSC
concentration of corn silage was found at the 118th day, but the highest at the 202th day (p<0.05). Silage pH values
were remained at normal levels. Flieg scores of corn silage were signifcantly affected by storage time. In this
research, changes were observed in nutrient composition and silage fermentation characteristics with the prolonged
storage time of corn silage made in mini silos in laboratory conditions

Anahtar Kelimeler

Mini silo, nutrient composition, silage, storage time, quality parameters

Laboratuvar Koşullarında Mini Siloda yapılan Mısır Silajının Besin Madde Kompozisyonu ve Silaj Kalite Parametrelerindeki Değişimi Üzerine Depolama Süresinin Etkisi

Öz

Bu çalışma; laboratuvar koşullarında plastik mini siloda yapılan mısır silajının, besin madde kompozisyonu ve
silaj kalite parametreleri üzerine depolama süresinin etkisini belirlemek amacıyla yapılmıştır. Silaj materyali olarak
mısır kullanılmıştır. % 31.87 Kuru madde (KM) içerikli Mısır hamur olumu devresinde hasat edilmiş, laboratuvar
koşullarında PVC mini silolarda 90, 104, 118, 132, 146, 150, 164, 178, 182, 196 ve 202 gün silolanmıştır. Silajın
depolama süresi uzadıkça kuru madde (KM) içeriğinde azalma görülmüştür. Silajın ham protein (HP), ham kül (HK)
ve ham sellüloz (HS) içeriğinde depolama süresi uzadıkça azalma, NÖM içeriğinde ise artış olmuştur (P<0.05).
Depolama süresi mısır silajının ham yağ (HY) içeriğinde önemli bir değişime neden olmamıştır. Silajın laktik
asit (LA) konsantrasyonu depolama süresinin 104. gününe kadar artmış, 104-188. günler arasında düşmüş, 188.
günden sonra tekrar artış göstermiştir (P<0.05). Depolama süresinin uzaması LA konsantrasyonunda dalgalanmaya
neden olmuştur. Depolama süresine bağlı olarak AA konsantrasyonu, LA konsantrasyonunun tam tersi bir durum
göstermiştir. Silajın NH3-N konsantrasyonu 146. günde en yüksek değeri göstermiş, CO2 konsantrasyonu 160.
güne kadar artmış sonra azalmıştır (P<0.05). Silajın SÇK konsantrasyonu en düşük 118. günde, en yüksek ise
202 günde elde edilmiştir. Silajın pH değeri her depolama periyodu için normal sınırlar arasında kalmıştır. Mısır
silajının Flieg puanı depolama zamanı ile etkilenmiştir. Bu çalışmada, laboratuvar koşullarında mini siloda yapılan
mısır silajının, depolama süresi uzadıkça besin madde kompozisyonu ve fermentasyon karakteristiklerinde değişim
olduğu görülmüştür.

Anahtar Kelimeler

Besin madde kompozisyonu, depolama zamanı, kalite parametreleri, mini silo, silaj

Kaynakça

• Akyildiz R, 1984. Laboratory quidance of feeds. A.Univ Agric Fac. Textbook:895 Practice Quid. 293p.
• AOAC, 1990. Association of Offcial Analytical Chemists. Offcial Methods of analysis, 15th (Ed.), Vol.1. AOAC, Washington, DC, 69-79.
• Archibald JD, 1946. Studies in the chemistry of grass silage. Agri. Res. 72(8); 277-288.
• Ashbell G, Weinberg ZG, Azrieli A, Hen Y, Horev B, 1991. A simple system to study the aerobic deterioaration of silages. Canadian Agric. Eng., 33: 391-393.
• Ashbell G, Weinberg ZG, Hen Y, 1998. Recycling of broiler litter for cattle feed. 10th European Poultry Congress, Jarusalem, Israel, 21-26.
• Ashbell G, Weinberg ZG, Hen Y, Filya İ, 2002. The effects of temparature on the aerobic stability of wheat or corn silages. J. India Microbiol Bioctechnol. 28: 261-263.
• Autrey KM, Knodt CB, Williams PS, 1947. Grass and legume silage studies using two-quart glass jars as miniature silos. J. Dairy Sci. 30(10):775-785.
• Buckmaster DR, Rotz CA, Muck RE, 1989. A comprehensive model of forage changes in the silo. Trans. ASAE 32(4): 1143-1152.
• Cabarkapa I, Palic D, Plavsic D, Vukmirovic DJ, Colovic R, 2010. The influence of a bacterial inoculant on reduction of aerobic microflora during ensiling of alfalfa. Food and Feed Research, 37(1): 23-26.
• Colovic R, Vukmirovic D, Palic D, Plavsic M, Glamocic D, Jajic I, Jeremic D, 2010. A simple system for studing the fermentation Dynamics of silages. Journal on Processing and Energy in Agriculture, 14(3): 157-159.
• Der Bedrosian MC, Kung Jr, l, Nestor Jr. KE, 2012. The effects of hybrid, maturity and length of storage on the composition and nutritive value of corn silage. J. Dairy. Sc.95: 5115-5126.
• Dubois M, Giles KA, Hamilton JK, Rebes PA and Smith F, 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem., 28: 350-356.
• Heinrichs J, Ishler V, 2010. Evaluating forage quality by visual appraisal, pH, and dry matter content. Department of Dairy and Animal Science The Pennsylvania State University 324 Henning Building University.
• Jaster EH, 1995. Legume and grass silage preservation. p. 91 in Post-harvest Physiology and Preservation of Forages. K. Moore and M. A. Peterson, ed. Crop Sci. Soc. of Am. Spec. Pubi. 22. Crop Sci. Soc. of Am. and Am. Soc. of Agron., Madison, WI.
• Johanson HE, Merry RJ, Davies DR, Kell DB, Theodorou MK, Griffth GW, 2005. Vacuum packing: a model system for laboratory-scale silage fermentations. Journal of Applied Microbiolojy, 98: 106-113.
• Kilic A, 2006. Determined of quality in roughage. Hasat Poblication, Istanbul.
• Kleinschmit DH, Kung lJr, 2006. The effects of Lactobacillus buchneri 40788 and Pediococcus pentosaceus R1094 on the fermentation of corn silage. J. Dairy Sci. 89: 3999-4004.
• Kolver ES, Roche JR, Mıller D, Densley R, 2001. Maize silage for dairy cows Proceedings of the New Zealand Grassland Association 63: 195.201.
• Kung L, DerBedrosian MD, 2010. Effect of Time of Storage on Changes in Nutrient Composition and Value of Silages. Penn State Dairy Cattle Nutrition Workshop.
• Lindgren S, Axelssona LT McFeeter RF, 1990. Anaerobic-lactate degradation by Lactobacillus plantarum. FEMS Microbiol. Lett. 66:209-213.
• Naeini SZ, Emami NK, Rowghani E, Bayat A, 2014. Influence of ensiling time on chemical composition, fermentation characteristics, gas production and protein fractions of sweet sorghum silage. Res. Opin. Anim.Vet.Sci. 4(6):286-203.
• Nevens WB, 1933. Types and varieties of corn for silage. University of Illinois Agricultural Experiment Station Bulletin, 391.
• Newbold JR, Lewis EA, Lavrijssen J, Brand HJ, Vedder H, Bakker J, 2006. Effect of storage time on ruminal starch degradability in corn silage. J. Dairy Sci. 89(Suppl 1):190. Abst.
• Palic D, Vukmirovic D, Colovic R, Ivanov D, Kokic R, Spasevski N, Jeremic D, 2011. Improved mini-silo for studying fermentation of silage in laboratory conditions. Biotechnology in Anim Husbandry, 27:3, 1271-1278
• Van Ranst G, Fievezb V, De Rieka J, Van Bockstaele E, 2009. Influence of ensiling forage at different dry matters and silage additives on lipid metabolism and fatty acid composition. Anim. Feed Sci. Techn. 150:62-74.
• Ward RT, Ondarza MB, 2008. Effect of month of sample submittal on corn silage nutrient fractions starch availability ,NDF digestibility, and fermentation profles measured at a commercial forage-testing laboratory. J. Dairy Sci. 91 (Suppl.1):30 abstr.
• Weinberg ZG, Khanal P, Yildiz C, Chen Y, Arieli A, 2010. Ensiling fermentation products and aerobic stability of corn and sorghum silages. Japanese Society of Grassland Science. 57:1-5.Winer
• BJ, Brown DR, Michels KM, 1991. Statistical Prenciples in Experiment Design. McGrow-Hill, Inc. USA,1048p.
• Winer BJ, Brown DR, Michels KM, 1991. Statistical Prenciples in Experiment Design. McGrow-Hill, Inc. USA,1048p.
• Yahaya MS, Kimura A, Harai J, Nguyen HV, Kawai M, Takahashi J, Matsuoka S, 2001. Effect of length of ensiling on silo degradation and digestibility of structural carbohydrates of lucerne and orchhardgrass. Anim. Feed Sci and Techn. 92:141-148.