Assessment of the Combined Effects of Acetamiprid and Propineb in Vivo

Yıl 2017, Cilt: 7 Sayı: 1, 79 - 86, 31.03.2017

Pınar Göç Rasgele

Öz

Pesticides are among commonly used chemicals in agriculture and are one of major environmental
pollutants. Acetamiprid and Propineb are widely used to control sucking insects and fungal infections on crops,
respectively. The study presented aimed to research genotoxic effects of mixture of Acetamiprid and Propineb,
in vivo. It was observed that mixture of Acetamiprid+Propineb increases the frequency of micronucleated
polychromatic erythrocytes (MNPCE) at all concentrations for 24 and 48 h depending on concentrations. But these
increases were not signifcant. The combined effect of the Acetamiprid and Propineb on bone marrow cells of
mice in vivo was found to be antagonistic in terms of percentage of MNPCE. In addition, mixture of Acetamiprid
and Propineb signifcantly decreased polychromatic erythrocytes/normochromatic erythrocytes (PCE/NCE) ratio
at all concentrations. The results of the present investigation revealed that Acetamiprid was non-genotoxic, while
mixture of the Acetamiprid and Propineb may have cytotoxic effects for mice bone marrow cells. But, additional in
vivo and in vitro mutagenicity studies measuring different levels of DNA damage are still necessary.

Anahtar Kelimeler

Mice bone marrow, micronucleus assay, mutagenicity, pesticide mixtur

Acetamiprid ve Propinebin Kombine Etkilerinin in Vivo Değerlendirilmesi

Öz

Pestisitler tarımda yaygın olarak kullanılan kimyasallardır ve önemli çevre kirleticilerindendirler.
Acetamiprid ve Propineb ürünlerde görülen fungal hastalıkları ve entomolojik patojenleri kontrol etmek için
yaygın olarak kullanılırlar. Bu çalışmada Acetamiprid ve Propineb pestisit karışımlarının genotoksik etkilerinin
in vivo araştırılması amaçlanmıştır. Acetamiprid+Propineb karışımının tüm konsantrasyonlarının 24 ve 48 saatlik
muamelelerde konsantrasyonlara bağlı olarak mikronukleuslu polikromatik eritrosit (MNPCE) frekansını arttırdığı
gözlendi. Fakat bu artışlar anlamlı bulunmamışlardır. Acetamiprid ve Propineb’in fare kemik iliği hücreleri üzerine
olan kombine etkisinin MNPCE yüzdesi bakımından antagonistik olduğu bulunmuştur. Ayrıca, Acetamiprid ve
Propineb karışımı polikromatik eritrosit/normokromatik eritrosit (PCE/NCE) oranını tüm konsantrasyonlarda
anlamlı olarak azaltmıştır. Çalışmanın sonuçları Acetamiprid’in non-genotoksik olduğunu, Acetamiprid ve
Propineb karışımının fare kemik iliği hücreleri için sitotoksik etkilere sahip olabileceğini göstermiştir. Fakat DNA
hasarının farklı seviyelerini ölçen ilave in vivo ve in vitro mutajenite çalışmaları yapılması gereklidir.

Anahtar Kelimeler

Fare kemik iliği, mikronukleus yöntemi, mutajenite, pestisit karışımı

Kaynakça

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