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Immunohistochemical Investigation in Sheep with Parasitary Liver Fibrosis

Yıl 2019, Cilt: 9 Sayı: 1, 244 - 253, 01.03.2019
https://doi.org/10.21597/jist.444133

Öz

Liver fibrosis significantly effect human and animal health in our country and worldwide. In this study, 30 sheep liver tissues with macroscopical parasitary fibrosis and 10 healthy liver tissues were taken in terms of histopathologically and immunohistochemically (α-SMA, iNOS COX-2, p53 and PCNA). Cell infiltration consisting of lymphocytes, macrophages and eosinophils and fibrosis were detected in portal area in histopatological investigation. And also there was nodules (pseudolobulus) formed by regenerated hepatocytes that do not vena centralis in center. Also granulomas contained in foreign body giant cells were seen. According to immunohistochemical examination; stronger positivity was observed in α-SMA, iNOS, COX-2, p53 and PCNA compared to control group. It has been found beneficial to use α-SMA to demonstrate connective tissue uptake in fibrosis, using COX-2 as due to fibrosis and cirrhosis at the base of liver tumors and their role in wound healing; using PCNA in order to evaluate increase proliferation, using p53 for apoptosis, using iNOS antibody as its secreted from macrophages in parasitic infections.

Kaynakça

  • Anavi S, Eisenberg-Bord M, Hahn-Obercyger M, Genin O, Pines M, Tirosh O, 2015. The role of iNOS in cholesterol-induced liver fibrosis, Laboratory Investigation, 95, 914-924.
  • Baddamwar A, 2004. Hepatic fibrosis in experimental Schistosom japonicum infection in pigs, Master of Science Programme in Veterinary Medicine for International Students Faculty of Veterinary Medicine and Animal Science Swedish University of Agricultural Sciences, Uppsala.
  • Balkaya İ, Kapakin Terim KA, Atasever İ, 2010. Fasciola hepatica ile doğal enfekte sığır karaciğerlerinin morfolojik ve histopatolojik olarak incelenmesi, Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 5, 1, 7-11.
  • Benzer F ve Temizer OS 2003. Fasciola hepatica ile enfekte koyunlarda lipid peroksidasyonu, antioksidant enzimler ve nitrik oksit düzeyleri, Turkish Journal of Veterinary and Animal Sciences, 27, 657-661.
  • Beytut E, Akça A, Gökçe Hİ, 2011. Pathological and immunohistochemical evaluation of the eff ects of interferon gamma (IFN-γ) and aminoguanidine in rats experimentally infected with Fasciola hepatica, Turkish Journal of Veterinary and Animal Sciences, 35, 4, 243-253.
  • Brunet LR, 2001. Nitric oxide in parasitic infections, International Immunopharmacology, 1, 1457-1467.
  • Cordova M, Reategui L ve Espinoza JR 1998. Immunodiagnosis of human Fascioliasis with Fasciola hepatica cysteine proteinases, Journal of Clinical Microbiology, 52, 3, 766-772.
  • Erdoğdu S, 2010. Safra yolu bağlanmış sıçanlarda antitrombotik ajan dalteparinin hepatik fibrozis gelişimini önlemedeki rolü. Trakya Üniversitesi, Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, Uzmanlık tezi (Basılmış).
  • Golbar HM, Izawa T, Juniantito V, Ichikawa C, Tanaka M, Kuwamura M, Yamate J, 2013. Immunohistochemical characterization of macrophages and myofibroblasts in fibrotic liver lesions due to Fasciola infection in cattle, Journal of Veterinary Medical Science, 75, 7, 857-65.
  • Ito Y, Yoshida H, Nakano K, Takamura Y, Miya A, Kobayashi K, Yokozawa T, Matsuzuka F, Matsuura N, Kuma K and Miyauchi A, 2003. Cyclooxygenase-2 expression in thyroid neoplasms, Histopathology, 42, 492-497.
  • Kumar V, Abbas AK, Fausto N, Astes JS, 2014. Robbins and cotran pathological basis of disease, Philadelphia US: Elsevier Saunders, 877-927.
  • Marinković D, Kukolj V, Aleksić-Kovačević S, Jovanović M, Knežević M, 2013. The role of hepatic myofibroblasts in liver cirrhosis in fallow deer (Dama dama) naturally infected with giant liver fluke (Fascioloides magna), BMC Veterinary Research, 6, 9, 45.
  • Özdemir E, 1998. Ürotelyal karsinogenezis araştırılmasında p53 anahtarı, Türk Klinik Tıp Bilimleri Dergisi, 18, 277-284.
  • Qi LN, Bai T, Chen ZS, Wu FX, Chen YY, De Xiang B, Peng T, Han ZG, Li LQ, 2014. The p53 mutation spectrum in hepatocellular carcinoma from Guangxi, China: role of chronic hepatitis B virus infection and aflatoxin B1 exposure, Liver Internation, 35, 3, 999-1009.
  • Rocha SWS, França M, Rodrigues GB, Barbosa KPS, Nunes AKS, Pastor AF, Oleveria AGV, Oleveria WH, Luna RLA, Peixoto CA, 2014. Diethylcarbamazine reduces chronic inflammation and fibrosis in carbon tetrachloride-(CCl 4-) Induced Liver Injury in Mice, Mediators of Inflammation, 696383, 15.
  • Rottenberg M, Castaños-Velez E, Mesquita R, Laguardia OG, Biberfeld P, Orn A, 1996. Intracellular co-localization of Trypanosoma cruzi and inducible nitric oxide synthase (İNOS): Evidence for dual pathway of iNOS induction, European Journalof Immunology, 26, 12, 3203-3213.
  • Santos FR, Vieira PM, Correa-Oliveira R, Giunchetti RC, Carneiro CM, Reis AB, Malaquias LC, 2011. Qualitative and quantitative immunohistochemical evaluation of iNOS expression in the spleen of dogs naturally infected with Leishmania chagasi, Parasitol Research, 108, 6, 1397-403.
  • Şahin İ, Yazar S ve Yaman O, 2008. Kayseri-karpuzsekisi havzasında yaşayan insanlarda Fasciola hepatica prevalansı, Journal of Health Sciences, 17, 2, 97-103.
  • Şimşek S, Çeribaşı AO, Ütük A, 2004. Dicrocoelium dendriticum’un koyun karaciğerinde yaptığı tahribatın morfolojik ve histopatolojik olarak incelenmesi, Türkiye Parazitoloji Dergisi, 28, 4, 189-191.
  • Taylor CR, Cote RJ, 1994. Immunomicroscopy: A diagnostic tool for the surgical pathologist, Saunders Company, 214-222.
  • Tousson E, Beltagy DM, Gazia MA, Al-Behbehani B, 2012. Expressions of p53 and CD 68 in mouse liver with Schistosoma mansoni infection and the protective role of silymarin, Toxicology and Industrial Health, 29, 8, 761–770.
  • WHO, 2014. Foodborne trematode infections. http://www.who.int/foodborne_trematode_infections/fascioliasis/en/ (Erişim Tarihi: 28.07.2015).
  • Wynn TA and Barron L, 2010. Macrophages: master regulators of inflammation and fibrosis, Semin. Liver Diseases, 30, 245–257.

Koyunlarda Paraziter Kaynaklı Karaciğer Fibrozisinde İmmunohistokimyasal İncelemeler

Yıl 2019, Cilt: 9 Sayı: 1, 244 - 253, 01.03.2019
https://doi.org/10.21597/jist.444133

Öz

Karaciğer fibrozisi, ülkemizde ve dünyanın pekçok bölgesinde insan ve hayvan sağlığını önemli ölçüde etkilemektedir. Bu çalışmada, makroskobik incelemede paraziter fibrozis bulguları gösteren 30 koyun karaciğeri histopatolojik ve immunohistokimyasal olarak (α-SMA, iNOS COX-2, p53 ve PCNA) incelendi. Histopatolojik incelemede portal alanlarda lenfosit, makrofaj ve eozinofilden oluşan hücre infiltrasyonu ile fibrozis, parankimde vena sentralisleri bulunmayan rejenere hepatositlerin oluşturduğu nodüller (pseudolobulus) şekillenmişti. Ayrıca bazı olgularda içinde yabancı cisim dev hücreleri bulunan granülomlar görüldü. İmmunohistokimyasal incelemede; kontrol grubuyla karşılaştırıldığında fibrotik karaciğerlerde α-SMA, iNOS, COX-2, p53 ve PCNA için çok daha yüksek pozitiflik gözlendi. Fibroziste bağ doku artışını göstermek için α-SMA, karaciğer tümörlerinin temelinde fibrozis ve sirozun olması ve yara iyileşmesinde rol alması yönünden COX-2, artan proliferasyonun değerlendirilmesinde PCNA, apoptozis için p53; paraziter enfeksiyonlarda makrofajlarda salınması yönünden iNOS antikorunun kullanılmasının faydalı olduğu görülmüştür.

Kaynakça

  • Anavi S, Eisenberg-Bord M, Hahn-Obercyger M, Genin O, Pines M, Tirosh O, 2015. The role of iNOS in cholesterol-induced liver fibrosis, Laboratory Investigation, 95, 914-924.
  • Baddamwar A, 2004. Hepatic fibrosis in experimental Schistosom japonicum infection in pigs, Master of Science Programme in Veterinary Medicine for International Students Faculty of Veterinary Medicine and Animal Science Swedish University of Agricultural Sciences, Uppsala.
  • Balkaya İ, Kapakin Terim KA, Atasever İ, 2010. Fasciola hepatica ile doğal enfekte sığır karaciğerlerinin morfolojik ve histopatolojik olarak incelenmesi, Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 5, 1, 7-11.
  • Benzer F ve Temizer OS 2003. Fasciola hepatica ile enfekte koyunlarda lipid peroksidasyonu, antioksidant enzimler ve nitrik oksit düzeyleri, Turkish Journal of Veterinary and Animal Sciences, 27, 657-661.
  • Beytut E, Akça A, Gökçe Hİ, 2011. Pathological and immunohistochemical evaluation of the eff ects of interferon gamma (IFN-γ) and aminoguanidine in rats experimentally infected with Fasciola hepatica, Turkish Journal of Veterinary and Animal Sciences, 35, 4, 243-253.
  • Brunet LR, 2001. Nitric oxide in parasitic infections, International Immunopharmacology, 1, 1457-1467.
  • Cordova M, Reategui L ve Espinoza JR 1998. Immunodiagnosis of human Fascioliasis with Fasciola hepatica cysteine proteinases, Journal of Clinical Microbiology, 52, 3, 766-772.
  • Erdoğdu S, 2010. Safra yolu bağlanmış sıçanlarda antitrombotik ajan dalteparinin hepatik fibrozis gelişimini önlemedeki rolü. Trakya Üniversitesi, Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, Uzmanlık tezi (Basılmış).
  • Golbar HM, Izawa T, Juniantito V, Ichikawa C, Tanaka M, Kuwamura M, Yamate J, 2013. Immunohistochemical characterization of macrophages and myofibroblasts in fibrotic liver lesions due to Fasciola infection in cattle, Journal of Veterinary Medical Science, 75, 7, 857-65.
  • Ito Y, Yoshida H, Nakano K, Takamura Y, Miya A, Kobayashi K, Yokozawa T, Matsuzuka F, Matsuura N, Kuma K and Miyauchi A, 2003. Cyclooxygenase-2 expression in thyroid neoplasms, Histopathology, 42, 492-497.
  • Kumar V, Abbas AK, Fausto N, Astes JS, 2014. Robbins and cotran pathological basis of disease, Philadelphia US: Elsevier Saunders, 877-927.
  • Marinković D, Kukolj V, Aleksić-Kovačević S, Jovanović M, Knežević M, 2013. The role of hepatic myofibroblasts in liver cirrhosis in fallow deer (Dama dama) naturally infected with giant liver fluke (Fascioloides magna), BMC Veterinary Research, 6, 9, 45.
  • Özdemir E, 1998. Ürotelyal karsinogenezis araştırılmasında p53 anahtarı, Türk Klinik Tıp Bilimleri Dergisi, 18, 277-284.
  • Qi LN, Bai T, Chen ZS, Wu FX, Chen YY, De Xiang B, Peng T, Han ZG, Li LQ, 2014. The p53 mutation spectrum in hepatocellular carcinoma from Guangxi, China: role of chronic hepatitis B virus infection and aflatoxin B1 exposure, Liver Internation, 35, 3, 999-1009.
  • Rocha SWS, França M, Rodrigues GB, Barbosa KPS, Nunes AKS, Pastor AF, Oleveria AGV, Oleveria WH, Luna RLA, Peixoto CA, 2014. Diethylcarbamazine reduces chronic inflammation and fibrosis in carbon tetrachloride-(CCl 4-) Induced Liver Injury in Mice, Mediators of Inflammation, 696383, 15.
  • Rottenberg M, Castaños-Velez E, Mesquita R, Laguardia OG, Biberfeld P, Orn A, 1996. Intracellular co-localization of Trypanosoma cruzi and inducible nitric oxide synthase (İNOS): Evidence for dual pathway of iNOS induction, European Journalof Immunology, 26, 12, 3203-3213.
  • Santos FR, Vieira PM, Correa-Oliveira R, Giunchetti RC, Carneiro CM, Reis AB, Malaquias LC, 2011. Qualitative and quantitative immunohistochemical evaluation of iNOS expression in the spleen of dogs naturally infected with Leishmania chagasi, Parasitol Research, 108, 6, 1397-403.
  • Şahin İ, Yazar S ve Yaman O, 2008. Kayseri-karpuzsekisi havzasında yaşayan insanlarda Fasciola hepatica prevalansı, Journal of Health Sciences, 17, 2, 97-103.
  • Şimşek S, Çeribaşı AO, Ütük A, 2004. Dicrocoelium dendriticum’un koyun karaciğerinde yaptığı tahribatın morfolojik ve histopatolojik olarak incelenmesi, Türkiye Parazitoloji Dergisi, 28, 4, 189-191.
  • Taylor CR, Cote RJ, 1994. Immunomicroscopy: A diagnostic tool for the surgical pathologist, Saunders Company, 214-222.
  • Tousson E, Beltagy DM, Gazia MA, Al-Behbehani B, 2012. Expressions of p53 and CD 68 in mouse liver with Schistosoma mansoni infection and the protective role of silymarin, Toxicology and Industrial Health, 29, 8, 761–770.
  • WHO, 2014. Foodborne trematode infections. http://www.who.int/foodborne_trematode_infections/fascioliasis/en/ (Erişim Tarihi: 28.07.2015).
  • Wynn TA and Barron L, 2010. Macrophages: master regulators of inflammation and fibrosis, Semin. Liver Diseases, 30, 245–257.
Toplam 23 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Yapısal Biyoloji
Bölüm Biyoloji / Biology
Yazarlar

Ayhan Akgün 0000-0001-6585-8655

Fatma İlhan 0000-0003-0363-6285

Yayımlanma Tarihi 1 Mart 2019
Gönderilme Tarihi 16 Temmuz 2018
Kabul Tarihi 5 Ekim 2018
Yayımlandığı Sayı Yıl 2019 Cilt: 9 Sayı: 1

Kaynak Göster

APA Akgün, A., & İlhan, F. (2019). Koyunlarda Paraziter Kaynaklı Karaciğer Fibrozisinde İmmunohistokimyasal İncelemeler. Journal of the Institute of Science and Technology, 9(1), 244-253. https://doi.org/10.21597/jist.444133
AMA Akgün A, İlhan F. Koyunlarda Paraziter Kaynaklı Karaciğer Fibrozisinde İmmunohistokimyasal İncelemeler. Iğdır Üniv. Fen Bil Enst. Der. Mart 2019;9(1):244-253. doi:10.21597/jist.444133
Chicago Akgün, Ayhan, ve Fatma İlhan. “Koyunlarda Paraziter Kaynaklı Karaciğer Fibrozisinde İmmunohistokimyasal İncelemeler”. Journal of the Institute of Science and Technology 9, sy. 1 (Mart 2019): 244-53. https://doi.org/10.21597/jist.444133.
EndNote Akgün A, İlhan F (01 Mart 2019) Koyunlarda Paraziter Kaynaklı Karaciğer Fibrozisinde İmmunohistokimyasal İncelemeler. Journal of the Institute of Science and Technology 9 1 244–253.
IEEE A. Akgün ve F. İlhan, “Koyunlarda Paraziter Kaynaklı Karaciğer Fibrozisinde İmmunohistokimyasal İncelemeler”, Iğdır Üniv. Fen Bil Enst. Der., c. 9, sy. 1, ss. 244–253, 2019, doi: 10.21597/jist.444133.
ISNAD Akgün, Ayhan - İlhan, Fatma. “Koyunlarda Paraziter Kaynaklı Karaciğer Fibrozisinde İmmunohistokimyasal İncelemeler”. Journal of the Institute of Science and Technology 9/1 (Mart 2019), 244-253. https://doi.org/10.21597/jist.444133.
JAMA Akgün A, İlhan F. Koyunlarda Paraziter Kaynaklı Karaciğer Fibrozisinde İmmunohistokimyasal İncelemeler. Iğdır Üniv. Fen Bil Enst. Der. 2019;9:244–253.
MLA Akgün, Ayhan ve Fatma İlhan. “Koyunlarda Paraziter Kaynaklı Karaciğer Fibrozisinde İmmunohistokimyasal İncelemeler”. Journal of the Institute of Science and Technology, c. 9, sy. 1, 2019, ss. 244-53, doi:10.21597/jist.444133.
Vancouver Akgün A, İlhan F. Koyunlarda Paraziter Kaynaklı Karaciğer Fibrozisinde İmmunohistokimyasal İncelemeler. Iğdır Üniv. Fen Bil Enst. Der. 2019;9(1):244-53.