Endemik Tür Geranium ibericum subsp. jubatum'un Farklı Kısımlarının Toplam Fenolik İçerikleri ve Antioksidan Aktivitelerinin Karşılaştırılması

Yıl 2020, Cilt: 10 Sayı: 1, 378 - 385, 01.03.2020

Emriye Ay Erhan Seyhan Gezen Rena Hüseyinoğlu

https://doi.org/10.21597/jist.553329

Öz

Bu çalışma, endemik tür Geranium ibericum subsp. jubatum’un fenolik içeriği ve antioksidan kapasitesi üzerine yapılmış ilk çalışmanın sonuçlarını içermektedir. Çalışmada, Türkiye için endemik tür olan G.ibericum subsp. jubatum’un farklı kısımlarının (yaprak, gövde, çiçek ve kök) metanol özütlerinin toplam fenolik içerikleri ve toplam antioksidan aktiviteleri belirlenmiş ve karşılaştırma yapılmıştır. G. ibericum subsp. jubatum’un farklı kısımlarına ait metanol özütlerinin antioksidan kapasiteleri sentetik bir antioksidan olan BHT ve doğal bir antioksidan olan α-tokoferol ile karşılaştırılmış ve sonuçlar değerlendirilmiştir. Toplam fenolik içerik ile toplam antioksidan kapasiteleri arasında pozitif bir ilişki bulunmaktadır. En yüksek fenolik içerik ve antioksidan kapasitesinin kök kısmının metanol özütüne ait olduğu bulunmuştur. Yapılan istatiksel değerlendirilmeler sonucunda G. ibericum subsp. jubatum’un farklı kısımları arasında toplam fenolik içerik ve toplam antioksidan aktiviteleri (DPPH, ABTS) yönünden önemli farklılıklar görülmüştür (p<0.05).

Anahtar Kelimeler

Geranium ibericum subsp. jubatum, ABTS, DPPH, antioksidan aktivitesi, toplam fenolik içerik

Comparison of Total Phenolic Contents and Antioxidant Activities of Different Parts of the Endemic Plant Geranium ibericum subsp. jubatum

Öz

This paper includes the results of the first study on the phenolic content and antioxidant capacity of endemic species Geranium ibericum subsp. jubatum found in Turkey. In this study, the methanol extracts of different parts (leaf, stem, flower, and root) of the Geranium ibericum subsp. jubatum exhibited different free radical scavenging activity, total phenolic content, and antioxidant activity. The synthetic antioxidant BHT and the natural antioxidant α-tocopherol were evaluated as a positive control and compared with methanol extracts of the plant parts. There was a positive correlation between the total phenolic content and the free radical scavenging activity in different parts of Geranium ibericum. It has been found that the highest phenolic content and antioxidant activity were found in the roots. The total phenolic content and antioxidant activity (ABTS, DPPH) showed statistically significant differences among the different parts of the G. ibericum subsp. jubatum (p<0.05).

Anahtar Kelimeler

Geranium ibericum subsp. jubatum, ABTS, DPPH, antioxidant activity, total phenolic content.

Kaynakça

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