Determination of Isoflavones in Nuts, Dried Fruits and Vegetables by High Performance Liquid Chromatography

Yıl 2020, Cilt: 10 Sayı: 2, 1191 - 1201, 01.06.2020

Teslima Daşbaşı

https://doi.org/10.21597/jist.619176

Öz

In this study, the amounts of daidzin, daidzein and genistein of isoflavone components were determined in nuts, dried fruits and vegetables by high performance liquid chromatography (HPLC). Firstly, the extraction conditions like the rate of the aqueous and organic phase, the total volume of the extraction solution, time and rotation per minute of centrifugation were optimized. After extraction conditions were specified, the parameters of HPLC device have investigated. The isoflavone components were extracted by a mixture of 0.1% acetic acid and methanol (20:80 v/v), at the room temperature. The chromatographic separation was conducted on C18 with wavelength of 260 nm at 30 oC. The diode array detector was used. The analytical parameters given as the dedection and quantification limits, linearity, precision and accuracy were determined. The relative standard deviation of method was determinated ≤3 % (n=21) and the calibration curse was worked in the range 1-100 mg L-1. The total isoflavone concentration in real samples from Sivas centrum was determined in the range 9.6 ˗ 469.1 μg g-1.

Anahtar Kelimeler

Isoflavone, Daidzin, Daidzein, Genistein, HPLC

Destekleyen Kurum

Sivas Cumhuriyet Üniversitesi Bilimsel Araştırma Projeler Birimi

Proje Numarası

GMYO-002

Teşekkür

Bu çalışma Sivas Cumhuriyet Üniversitesi Bilimsel Araştırma Projeler Birimi tarafından desteklenmiştir.

Determination of Isoflavones in Nuts, Dried Fruits and Vegetables by High Performance Liquid Chromatography

Öz

In this study, the amounts of daidzin, daidzein and genistein of isoflavone components were determined in nuts, dried fruits and vegetables by high performance liquid chromatography (HPLC). Firstly, the extraction conditions like the rate of the aqueous and organic phase, the total volume of the extraction solution, time and rotation per minute of centrifugation were optimizaed. After extraction conditions were specified, the parameters of HPLC device have investigated. The isoflavone components were extracted by a mixture of 0.1% acetic acid and methanol (20:80 v/v), at the room temperature. The chromatographic separation was conducted on C18 with wavelength of 260 nm at 30 oC. The diode array detector was used. The analytical parameters given as the dedection and quantification limits, linearity, precision and accuracy were determined. The relative standard deviation of method was determinated ≤3 % (n=21) and the calibration curse was worked in the range 1-100 mg L-1. The total isoflavone concentration in real samples from Sivas centrum was determined in the range 9.6 ˗ 469.1 μg g-1.

Anahtar Kelimeler

Isoflavone, Daidzin, Daidzein, Genistein, HPLC

Proje Numarası

GMYO-002

Kaynakça

• Abrankó L, Nagy A, Szilvássy B, Stefanovits-Bányai E, Hegedüs A, 2015. Genistein isoflavone glycoconjugates in sour cherry (Prunus cerasus L.) cultivars. Food Chemistry,166: 215–222.
• Barros F, Dykes L, Awika JM, Rooney LW, 2013. Accelerated solvent extraction of phenolic compounds from sorghum brans. Journal of Cereal Science, 58: 305–312.
• Bingham SA, Atkinson C, Liggins J, Bluck L,Coward A, 1998. Phytooestrogens: whereare we now? British Journal of Nutrition, 79: 393–406.
• Brglez Mojzer E, Knez Hrncic M, Skerget M, Knez Z, Bren U, 2016. Polyphenols: Extraction methods, antioxidative action, bioavailability and anticarcinogenic effects. Molecules, 2: 901.
• Cos P, Bruyne TD, Apers S, Berghe DV, Pieters L, Vlietinck AJ, 2003. Phytoestrogens: Recent Developments. Planta Medica, 69: 589–599.
• Delgado-Zamarreno MM, Perez-Martin L, Bustamante-Rangel M, Carabias-Martinez R, 2012. A modified QuErChers method as a sample treatment before the determination of isoflavones in foods by ultra-performance liquid chromatography–triple quadrupole mass spectrometry. Talanta, 100: 320–32.
• Delmonte P, Perry J, Rader JI, 2006. Determination of isoflavones in dietary supplements containing soy, Red Clover and Kudzu: Extraction followed by basic or acid hydrolysis. Journal of Chromatography A, 1107: 59–69.
• Durmaz G, Gökmen V, 2010. Impacts of roasting oily seeds and nuts on their extracted oils. Lipid Technology, 22(8): 179–182.
• Grace PB, Taylor JI, Botting NP, Fryatt T, Oldfield MF, Bingham SA, 2003. Quantification of isoflavones and lignans in urine using gas chromatography/mass spectrometry. Analytical Biochemistry, 315: 114–121.
• Franke AA, Custer LJ, Wilkens LR, Marchand LL, Nomura AMY, Goodman MT, Kolonel LN, 2002. Liquid chromatographic- photodiode array mass spectrometric analysis of dietary phytoestrogens from human urine and blood. Journal of Chromatography B, 777: 45–59.
• Fukuda I, Tsutsui M, Yoshida T, Toda T, Takanori T, Ashida H, 2011. Oral Toxicological Studies of Black Soybean (Glycine Max) Hull Extract: Acute Studies in Rats and Mice, And Chronic Studies in Mice. Food and Chemical Toxicology, 49(12): 3272–3278.
• King JC, Rechkemmer G, Constance JG, 2008. Second International Nuts and Health Symposium, 2007: Introduction. The Journal of Nutrition, 138( 9): 17345–17355.
• Klejdus B, Vacek J, Adam V, Zehnalek J, Kizek R, Trnkova L, Kuban V, 2004. Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection. Journal of Chromatography B, 806: 101–111.
• Krenn L, Unterrieder I, Ruprechter R, 2002. Quantification of isoflavones in red clover by high-performance liquid chromatography. Journal of Chromatography B, 777: 123–128.
• Küçükboyacı N, Kadıoğlu O, Adıgüzel N, Tamer U, Güvenç A, Bani B, 2013. Determination of Isoflavone Content by HPLC-UV Method and in vitro Antioxdant Activity of Red Clover (Trifolium Pratense L.). Turkish Journal of Pharmceutical Sciences, 10(3): 463–472.
• Liggins J, Bluck LJ, Runswick S, Atkinson C, Coward WA, Bingham SA, 2000. Daidzein and genistein contents of vegetables. British Journal of Nutrition, 84(5): 717–725.
• Maubach J, Bracke ME, Heyerick A, Depypere HT, Serreyn RF, Mareel MM, Keukeleire DD, 2003. Analytical Technologies in the Biomedical and Life Sciences. Quantitation of soy-derived phytoestrogens in human breast tissue and biological fluids by high-performance liquid chromatography. Journal of Chromatography B, 784: 137–144.
• Modaseri M, Messripour M, Khorami H, 2011. Effect of Soybean on Male Reproductive Physiology in Mice. International Conference on Life Science and Technology, 3: 15–18.
• Naim M, Gestetner B, Zilkah S, Birk Y, and Bondi A, 1974. Soybean Isoflavones. Characterization, Determination, and Antifungal Activity. Journal of Agricultural and Food Chemistry, 22: 806–810.
• Luque-Garcia JL, Luque de Castro MD, 2004. Ultrasound-assisted Soxhlet extraction: an expeditive approach for solid sample treatment. Aplication to the extraction of total fat from oleaginous seeds. Journal of Chromatography A, 1034: 237–242.
• Özhatay N, Kültür Ş, Arslan S, 2009. Check-list of additional taxa to the supplement Flora of Turkey IV. Turkish Journal of Botany, 33: 191–226.
• Prior RL, Cao G,1999. Assessing antioxidant capacity in plant foods. Free Radical Biology & Medicine, 27: S11–S.
• Prior RL, Wu X, Schaich K, 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53: 4290–302.
• Ribeiro MLL, Mandarino JMG, Carrao-Panizzi MC, Oliveira MCN, Campo CBH, Nepomuceno AL, Ida EI, 2007. Isoflavone content and b-glucosidase activity in soybean cultivars of different maturity groups. Journal of Food Composition and Analysis, 20: 19–24.
• Rostagno MA, Villares A, Guillamon E, García-Lafuente A, Martínez, JA, 2009. Sample preparation for the analysis of isoflavones from soybeans and soy foods. Journal of Chromatography A, 1216: 2–29.
• Stratil P, Klejdus B, Kuban V,2006. Determination of totalcontent of phenolic compounds and their antioxidantactivity in vegetables - Evaluation of spectrophotometricmethods. Journal of Agricultural and Food Chemistry, 54:607–616.
• Thomas BF, Zeisel SH, Busby MG, Hill JM, Mitchell RA, Scheffler NM, Brown SS, Bloeden LT, Dix KJ, Jeffcoat AR, 2001. Quantitative analysis of the principle soy isoflavones genistein, daidzein and glycitein, and their primary conjugated metabolites in human plasma and urine using reversed-phase high-performance liquid chromatography with ultraviolet detection. Journal of Chromatography B, 760: 191–205.
• Toro-Funes N, Odriozola-Serrano I, Bosch-Fuste J, Latorre-Moratalla ML, Veciana-Nogues MT, Izquierdo-Pulido M, Vidal-Carou MC, 2012. Fast simultaneous determination of free and conjugated isoflavones in soy milk by UHPLC–UV. Food Chemistry, 135: 2832–2838.
• Tsao R, 2010. Chemistry and biochemistry of dietary polyphenols. Nutrients, 2: 1231–1246.
• Vila-Donat P, Caprioli G, Maggi F, Ricciutelli M, Torregiani E, Vittori S, Sagratini G, 2015. Effective clean-up and ultra high-performance liquid chromatography–tandem mass spectrometry for isoflavone determination in legumes. Food Chemistry, 174: 487–494.
• Xiao HB, Krucker M, Albert K, Liang XM, 2004. Determination and identification of isoflavonoids in Radix astragali by matrix solid-phase dispersion extraction and high-performance liquid chromatography with photodiode array and mass spectrometric detectionJournal of Chromatography A, 1032: 117–124.
• Zafra-Gómez A, Garballo A, García-Ayuso LE, Morales Juan C, 2010. Improved sample treatment and chromatographic method for the determination of isoflavones in supplemented foods. Food Chemistry, 123: 872–877.