Peroxidases are enzymes that catalyze the oxidation of various organic substrates in the presence of hydrogen peroxide and contain heme group in their structure. In particular, plant peroxidases are frequently used in industry, clinical diagnosis, biosensor construction and organic synthesis reactions. Due to their commercial value, the identification and purification of these enzymes in different sources is of great importance. In this study, peroxidase enzyme was purified for the first time by using aminobenzohydrazide-based affinity chromatography technique from onion roots 750-fold with a yield of 37.7. SDS-PAGE was performed to determine the molecular weight of the purified enzyme and a single band was observed at 51.2 kDa. In addition, KM values of the enzyme for guaiacol, ABTS and pyrogallol substrates were calculated as 3.44, 0.46 and 21.27 mM, respectively.
Onion (Allium cepa) root peroxidase affinity chromatography purification
Soğan (Allium cepa) kökü peroksidaz afinite kromatografisi saflaştırma
Birincil Dil | Türkçe |
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Konular | Kimya Mühendisliği |
Bölüm | Kimya / Chemistry |
Yazarlar | |
Yayımlanma Tarihi | 1 Haziran 2020 |
Gönderilme Tarihi | 20 Ekim 2019 |
Kabul Tarihi | 1 Şubat 2020 |
Yayımlandığı Sayı | Yıl 2020 Cilt: 10 Sayı: 2 |