Investigation of Neurotoxic and Immunotoxic Effects of the Chinar (Platanus orientalis L.) Tree Leaf Infusion Against Ethanol Toxicity in Rats

Yıl 2020, Cilt 10, Sayı 2, 778 - 787, 01.06.2020

Abdulahad DOĞAN Fatih DONMEZ Abdulhamit BATTAL Ali ASLAN Ozgur Ozan ANUK

https://doi.org/10.21597/jist.643987

Cited By: 1

Öz

In this study, immunotoxic and neurotoxic effects of leaf infusion of Platanus orientalis L. (PO) were investigated in rats ethanol-induced toxicity. Neurotoxic effects of ethanol were evaluated by measuring acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in rat brain tissue. Immunotoxic effects were evaluated by adenosine deaminase (ADA) and myeloperoxidase (MPO) biomarkers in rat liver, lung, erythrocyte and spleen tissues. Thirty male Wistar rats were divided into five groups after toxicity study. The groups are explained as follow: Control, Ethanol 20 %, Ethanol 20 % + Silymarin (10 mg kg-1), Ethanol 20 % + PO-20 mg mL-1 infusion and Ethanol 20 % + PO-60 mg mL-1 infusion. The results showed that AChE and BChE activities statistically decreased in the groups treated with PO leaf infusion. While there was a statistically significant decrease in ADA and MPO activities in liver tissue of groups treated with PO leaf infusion. Also, MPO activity of erythrocyte in ethanol group was significantlly increased according to silymarin and PO-60 groups. On the other hand, there was no statistically significant finding was found in lung and spleen tissues. It was concluded that the infusion prepared from P. orientalis leaves suppressed to ethanol-induced neurotoxicity and immunotoxicity.

Anahtar Kelimeler

Platanus orientalis, acetylcholinesterase, butyrylcholinesterase, adenosine deaminase, myeloperoxidase, ethanol

Kaynakça

• Abdalla FH, Cardoso AM, Schmatz R, Gonçalves JF, Baldissarelli J, Martins CC, Pereira LB, Zanini D, de Oliveira LS, da Costa P, Pimentel VC, Pereira LB, Lhamas CL, Schetinger MR, Morsch VM, Mazzanti CM, 2014. Protective effect of quercetin in ecto-enzymes, cholinesterases, and myeloperoxidase activities in the lymphocytes of rats exposed to cadmium. Molecular and Cellular Biochemistry, 396(1-2): 201-211.
• Asadbeigi M, Mohammadi T, Rafieian-Kopaei M, Saki K, Bahmani M, Delfan, M, 2014. Traditional effects of medicinal plants in the treatment of respiratory diseases and disorders: an ethnobotanical study in the Urmia. Asian Pacific Journal of Tropical Medicine, 7: 364–368.
• Bilgi C, Tokgöz S, Aydin A, Çelik T, Uzbay IT, 2003. The effects of chronic ethanol consumption and ethanol withdrawal on serum cholinesterase activity in rats. Alcohol, 38(4): 316-320.
• Bradley PP, Priebat DA, Christensen RD, Rothstein G, 1982. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. Journal of Investigative Dermatology, 78(3): 206-209.
• Brust J, 2010. Ethanol and cognition: indirect effects, neurotoxicity and neuroprotection: a review. International Journal of Environmental Research and Public Health, 7(4): 1540-1557.
• Cargnelutti LO, Bitencourt PER, Bochi G, Duarte T, Boligon A, Pigatto AS, Athayde ML, Moresco RN, Moretto MB. 2015. Syzygium cumini Leaf Extract Protects Against Ethanol Induced Acute Injury in Rats by Inhibiting Adenosine Deaminase Activity and Proinflammatory Cytokine Production. Research Journal of Phytochemistry, 9: 56-67.
• Celik I, Isık I, Kaya MS, 2010. Evaluation of neurotoxic and immunotoxic effects of trichloroacetic acid on rats. Toxicology and Industrial Health, 26(10): 725-731.
• Celik I, Isik I, Ozok N, Kaya MS, 2011. Neurotoxic and immunotoxic effects of fenthion and omethoate on frogs at acute exposure. Toxicology and Industrial Health, 27(4): 357-362.
• Chadwick SMS, Sisson JH, 2015. Alcohol’s effects on lung health and immunity. Alcohol Research, 37(2): 199.
• Chatzigeorgiou S, Thai QD, Tchoumtchoua J, Tallas K, Tsakiri EN, Papassideri I, Trougakos IP, 2017. Isolation of natural products with anti-ageing activity from the fruits of Platanus orientalis. Phytomedicine, 33: 53-61.
• Demartini G, Esposti D. 2002. Pharmacology of Silymarin. Clinical Drug Investigation, 22: 51-65.
• Dogan A, Anuk OO, 2019. Investigation of the phytochemical composition and antioxidant properties of chinar (Platanus orientalis L.) leaf infusion against ethanol-induced oxidative stress in rats. Molecular Biology Reports, 46(3): 3049-3061.
• Eardley S, Bishop FL, Prescott P, Cardini F, Brinkhaus B, Santos-Rey K, Vas J, Von Ammon K, Hegyi G, Dragan S, Uehleke B, Fønnebø V, Uehleke B, 2012. A systematic literature review of complementary and alternative medicine prevalence in EU. Journal of Complementary Medicine Research, 19(2): 18-28.
• Ellman GL, Courtney KD, Andres JV, Featherstone RM, 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7(2): 88-95.
• Fernández E, Rodrigo L, Riestra S, García S, Gutiérrez F, Ocio G. 2000. Adenosine deaminase isoenzymes and neopterin in liver cirrhosis. Journal of Clinical Gastroenterology, 30(2): 181-186.
• George AK, Behera J, Kelly KE, Zhai Y, Tyagi N, 2017. Hydrogen sulfide, endoplasmic reticulum stress and alcohol mediated neurotoxicity. Brain Research Bulletin, 130: 251-256.
• Gezginci-Oktayoglu S, Sacan O, Bolkent S, Ipci Y, Kabasakal L, Sener G, Yanardag R. 2014. Chard (Beta vulgaris L. var. cicla) extract ameliorates hyperglycemia by increasing GLUT2 through Akt2 and antioxidant defense in the liver of rats. Acta Histochemica, 116(1): 32-39
• Giusti G, Bergmeyer HU, 1974. Handbook of enzymatic methods of analysis. New York Academic Press NO:2, pp. 1092–1099. New York-USA.
• Gulec M, Iraz M, Yilmaz HR, Ozyurt H, Temel I, 2006. The effects of ginkgo biloba extract on tissue adenosine deaminase, xanthine oxidase, myeloperoxidase, malondialdehyde, and nitric oxide in cisplatin-induced nephrotoxicity. Toxicology and Industrial Health, 22(3): 125-130.
• Gurib-Fakim A, 2006. Medicinal plants: traditions of yesterday and drugs of tomorrow. Molecular Aspects of Medicine, 27(1):1-93.
• Gülçin İ, Scozzafava A, Supuran CT, Koksal Z, Turkan F, Çetinkaya S, Alwasel SH, 2016. Rosmarinic acid inhibits some metabolic enzymes including glutathione S-transferase, lactoperoxidase, acetylcholinesterase, butyrylcholinesterase and carbonic anhydrase isoenzymes. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(6): 1698-1702.
• Haider S, Nazreen S, Alam MM, Hamid H, Alam MS, 2012. Anti-inflammatory and anti-nociceptive activities of Platanus orientalis Linn. and its ulcerogenic risk evaluation. Journal of Ethnopharmacology, 143(1): 236-240.
• Hajhashemi V, Ghannadi A, Mousavi S, 2011. Antinociceptive study of extracts of Platanus orientalis leaves in mice. Research in Pharmaceutical Sciences, 6(2): 123–128.
• Hosseini SM, Taghiabadi E, Abnous K, Hariri AT, Pourbakhsh H, Hosseinzadeh H, 2017. Protective effect of thymoquinone, the active constituent of Nigella sativa fixed oil, against ethanol toxicity in rats. Iranian Journal of Basic Medical Sciences, 20(8): 927.
• Jebali J, Khedher SB, Sabbagh M, Kamel N, Banni M, Boussetta H, 2013. Cholinesterase activity as biomarker of neurotoxicity: utility in the assessment of aquatic environment contamination. Journal of Integrated Coastal Zone Management, 13(4): 525-537.
• Khan AS, 2017. Woody plants with possible anti-HIV activity. In Medicinally Important Trees. Springer Cham NO:1, pp. 109-131, Gewerbestrasse- Switzerland.
• Kocaman N, Özlem D. 2015. Hepatoprotektif bir ajan: Silymarin. Firat Medical Journal, 2015; 20(3): 128-32.
• Lu N, Sui Y, Tian R, Peng YY, 2018. Inhibitive effects of quercetin on myeloperoxidase-dependent hypochlorous acid formation and vascular endothelial injury. Journal of Agricultural and Food Chemistry, 66(19): 4933-4940.
• Meisel P, Krause T, Cascorbi I, Schroeder W, Herrmann F, John U, Kocher T, 2002. Gender and smoking-related risk reduction of periodontal disease with variant myeloperoxidase alleles. Genes and Immunity, 3(2): 102.
• Mitchell JJ, Paiva M, Heaton MB, 1999. The antioxidants vitamin E and β-carotene protect against ethanol-induced neurotoxicity in embryonic rat hippocampal cultures. Alcohol, 17(2): 163-168.
• Mitrokotsa D, Mitaku S, Demetzos C, Harvala C, Mentis A, Perez S, Kokkinopoulos D, 1993. Bioactive compounds from the buds of Platanus orientalis and isolation of a new kaempferol glycoside. Planta Medica, 59(06): 517-520.
• Nagy LE, Diamond I, Casso DJ, Franklin C, Gordon AS, 1990. Ethanol increases extracellular adenosine by inhibiting adenosine uptake via the nucleoside transporter. Journal of Biological Chemistry, 265(4): 1946-1951.
• Nixon K, Crews FT, 2002. Binge ethanol exposure decreases neurogenesis in adult rat hippocampus. Journal of Neurochemistry, 83(5): 1087-1093.
• Olson JR, 2018. Biomarkers of chlorpyrifos and profenofos exposure and effect in adult and adolescent egyptian cotton field workers. Occupational and Environmental Medicine, 75(2): 287.
• Ozkol H, Bulut G, Balahoroglu R, Tuluce Y, Ozkol HU. 2017. Protective effects of selenium, N-acetylcysteine and vitamin E against acute ethanol intoxication in rats. Biological Trace Element Research, 175(1): 177-185.
• Patočka J, Kuča K, Jun D, 2004. Acetylcholinesterase and butyrylcholinesterase–important enzymes of human body. Acta Medica (Hradec Králové), 47(4): 215-228.
• Rix M, Fay MF, 2017. Platanus orientalis: Platanaceae. Curtis's Botanical Magazine, 34(1): 29-40.
• Shende S, Joshi KA, Kulkarni AS, Charolkar C, Shinde VS (2018). Platanus orientalis Leaf Mediated Rapid Synthesis of Catalytic Gold and Silver Nanoparticles. Journal of Nanomedicine and Nanotechnology, 9(494): 2.
• Tamura R, Ohta H, Satoh Y, Nonoyama S, Nishida Y, Nibuya M, 2016. Neuroprotective effects of adenosine deaminase in the striatum. Journal of Cerebral Blood Flow & Metabolism, 36(4): 709-720.
• Tantry MA, Akbar S, Dar JA, Irtiza S, Galal A, Khuroo MA, Ghazanfar K, 2012. Acylated flavonol glycoside from Platanus orientalis. Fitoterapia, 83(2): 281-285.
• Tonk EC, De Groot DM, Wolterbeek AP, Penninks AH, Waalkens-Berendsen ID, Piersma AH, Van Loveren H, 2013. Developmental immunotoxicity of ethanol in an extended one-generation reproductive toxicity study. Archives of Toxicology, 87(2): 323-335.
• Tuzlacı E, Erol MK, 1999. Turkish folk medicinal plants. Part II: Eğirdir (Isparta). Fitoterapia, 70(6): 593-610.