The Determination of Inhibition Effect of Extracts of Thymbra sintenisii Bornm. et Aznav. Subsp on Angiotensin Converting Enzyme

Yıl 2020, Cilt: 10 Sayı: 3, 1848 - 1856, 01.09.2020

Tuğba Gür , Fatih Karahan , Zehra Baş , Vedat Türkoğlu

https://doi.org/10.21597/jist.702359

Cited By: 2

Öz

Angiotensin converting enzyme (ACE, peptidyldipeptidase A, EC 3.4.15.1) has got a significant role in the arrangement of blood pressure. ACE inhibitors usually play a part in the therapies of hypertension. Hypertension is an significant cardiovascular risk factor. The main purpose of the treatment is to reduce the incidence of hypertension. In this study, the inhibition effect of butanol and water extracts of Thymbra sintenisii Bornm. et Aznav. Subsp on angiotensin converting enzyme (ACE) activity in human plasma was investigated. ACE activity was calculated at 345 nm spectrophotometrically. Extracts of Thymbra sintenisii Bornm. et Aznav. subsp plant with water and butanol were made. The effectiveness of these extracts on ACE activity was researched. Water and butanol extracts of Thymbra sintenisii indicated inhibition impact on ACE. IC50 values for water and butanol extracts of Thymbra sintenisii was measured to be 1.696 mg mL-1 and 0.395 mg mL-1 respectively. Inhibition type for water and butanol extracts of Thymbra sintenisii from Lineweaver-Burk chart was defined to be non-competitive inhibition. Synthetic ACE inhibitors are utilized in the therapy of hypertension. On the other hand, synthetic ACE inhibitors exhibit a large number of adverse effects. Therefore, ACE inhibitors have been newly researched from native herbs. These conclusions demonstrate that water and butanol extracts of Thymbra sintenisii plant may have an ACE inhibition capacity.

Anahtar Kelimeler

Angiotensin converting enzyme, Inhibition, Thymbra sintenisii, Hypertension

Kaynakça

• Acharya J, Karak S, De B,2016. Metabolite Profile and Bioactivity of Musa X Paradisiaca L. Flower Extracts. J. Food Biochem, 40:724–730.
• Akkol EK, Avci G, Kucukkurt I, Keles H, Tamer U, Ince S, Yesilada E, 2009. Cholesterol-reducer, antioxidant and liver protective effects of Thymbra spicata L. var. Spicata. J Ethnopharmacol, 126:314-319.
• Andújar-Sánchez M, Cámara-Artigas A, Jara-Pérez V, 2003. Purification of angiotensin 1 converting enzyme from pig lung using concanavalin-A sepharose chromatography. Journal of Chromatography B, 783:247-252.
• Basi Z , Turkoglu N, Turkoglu V, Karahan F, 2019. In vitro effect of ethyl acetate, butanol and water extracts of Juniperus excelsa Bieb. on Angiotensin Converting Enzyme purified from human plasma. Chemical Papers, 73:2525–2533.
• Basi Z, Turkoglu V, 2018. Purification of angiotensin-converting enzyme from human plasma and investigation of the effect of some active ingredients isolated from Nigella sativa L. Extract on the enzyme activity. Biomedical Chromatography, 32: 4175.
• Basi Z, Turkoglu V, 2019. In vitro effect of oxidized and reduced glutathione peptides on angiotensin converting enzyme purified from human plasma. Journal of Chromatography B, 1104:190–195.
• Carretero OA, Oparil S, 2000. Essential hypertension. Part I: definition and etiology. Circulation, 101:329–335.
• Dang Y, Zhou T, Hao L, Cao J, Sun Y, Pan D, 2019. In vitro and in vivo Studies on the angiotensin-converting enzyme inhibitory activity peptides isolated from broccoli protein hydrolysate. Journal of Agricultural and Food Chemistry, 67(24): 6757-6764.
• Duncan AC, Jager AK, Van Staden J, 1999. Screening of Zulu medicinal plants for angiotensin converting enzyme (ACE) inhibitors, J Ethnopharmacol, 68:63–70.
• Fakıllı O,Özgüven M,2012. Inventory of researches on thyme (Thymus vulgaris L) in Turkey. ÇÜ Fen Mühendislik Bil Derg, 27:54-66.
• Holmquist B, Bünning P, J.F. Riordan JF, 1979. A continuous spectrophotometric assay for angiotensin converting enzyme. Analytical Biochemistry, 95:540-548.
• Israili ZH, Hall WD,1992. Cough and angioneurotic edema associated with angiotensin-converting enzyme inhibitor therapy, A review of the literature and pathophysiology. Ann Inter Med, 117:234-42.
• Jimsheena VK, Gowda LR, 2011. Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides Derived from Arachin by Simulated Gastric Digestion. Food Chem, 125:561–569.
• Kearney PM, Whelton M , Reynolds K, Muntner P, Whelton PK , He J, 2005. Global burden of hypertension: analysis of worldwide data. Lancet, 365: 217–223.
• Koparal AT, Zeytinoğlu M ,2003. Effects of carvacrol on a human non-small cell lung cancer (NSCLC) cell line A549. Cytotechnolog, 43:149-154.
• Leong XF, Mustafa MR, Jaarin K, 2013. Nigella sativa and its protective role in oxidative stress and hypertension. Evidence based Complementary and Alternative Medicine, 2013:120732.
• Li GH, Le GW, Shi YH, Shrestha S, 2003. Angiotensin I – converting enzyme inhibitory peptides derived from food proteins and their physiological and pharmacological effects. Nutr. Res, 24: 469–486.
• Loizzo MR , Saab AM, Tundis R , Menichini F, Bonesi M, Piccolo V, Statti GA, de Cindio B, Houghton PJ, Menichini F, 2008. In vitro inhibitory activities of plants used in Lebanon traditional medicine against angiotensin converting enzyme (ACE) and digestive enzymes related to diabetes. J Ethnopharmacol., 119:109-16.
• Namjoyan F, Azemi ME, Abdollahi E, Goudarzi N, Nikan K, 2015. Angiotensin I Converting Enzyme Inhibitory Activities of Hydroalcoholic Extracts of Nardostachys jatamansi, Prangos ferulacea and Marrubium vulgare. Jundishapur J Nat Pharm Prod, 10:17255.
• Onat T, Emerk K, Sözmen E, 2006. İnsan Biyokimyası. Palme Yayıncılık, s.813, Ankara.
• Prasanth RV, Ravi Vital K , Varsha P, Satyan S , 2014. Review on Thymus vulgaris traditional uses and pharmacological properties. Med Aromat Plants, 3:164-167.
• Raji IA, Mugabo P, Obikeze K, 2012. Effect of Tulbaghia violacea on the blood pressure and heart rate in male spontaneously hypertensive Wistar rats. J Ethnopharmacol, 140:98–106.
• Ranilla LG, Kwon VI, Apostolidis E , Shetty K , 2010. Phenolic compounds, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension of commonly used medicinal plants, herbs and spices in Latin America. Bioresour Technol, 101:4676–89.
• Sasaki C, Tamura S, Tohse R, Fujita S, Kikuchi M, Asada C, Nakamura Y, 2019. Isolation and identification of an angiotensin I-converting enzyme inhibitory peptide from pearl oyster (Pinctada fucata) shell protein hydrolysate. Process Biochemistry, 77: 137-142.
• Siti HN, Kamisah Y, Nur Iliyani MI, Mohamed S, Jaarin K, 2017. Citrus Leaf Extract Reduces Blood Pressure and Vascular Damage in Repeatedly Heated Palm Oil Diet-Induced Hypertensive Rats. Biomed. Pharmacother, 87:451–460.
• Zhang P, Chang C, Liu H, Li B, Yan Q, Jiang Z, 2020. Identification of novel angiotensin I-converting enzyme (ACE) inhibitory peptides from wheat gluten hydrolysate by the protease of Pseudomonas aeruginosa. Journal of Functional Foods. 65: 103751.