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Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province

Yıl 2020, Cilt: 10 Sayı: 4, 2366 - 2377, 15.12.2020

Mustafa Usta , Abdullah Güller

https://doi.org/10.21597/jist.719099
Cited By: 3

Öz

Virus-like symptoms were observed in alfalfa plants (Medicago sativa L.) grown in Van province of Turkey in 2019. The symptoms observed were dwarfing, mosaic pattern and yellowing of leaves, rosetting, and decreasing in leaf sizes. Genomic RNA of 19 symptomatic and non-symptomatic alfalfa leaves were extracted for the detection of virus RNA using virus-specific primer pair by RT-PCR. Of the 19 alfalfa leaf specimens, the six yielded the expected 700 bp DNA band in severely symptomatic alfalfa specimens. Two of them randomly selected were inserted into a plasmid vector. Obtained recombinant plasmids were sequenced in both directions. According to the results of the sequence analysis, it was revealed that the virus in infected alfalfa was the Alfalfa mosaic virus. The sequence data were recorded into the GenBank with access numbers MT210179 and MT210178 and denominated as Alakoy Y9 and Alakoy Y1 isolates, respectively. According to the phylogenetic tree created with 16 AMV sequences registered in the GeneBank for both sequences, the AMV CP gene sequence indicated the highest similarity with USA (JN256023), Brazil (FJ858265), and Puglia (Y09110) isolate and the lowest with Korea isolate (KY348844), at the nucleotide level. It was also revealed that both sequences show 98.45% nucleotide similarity with 7 nucleotide exchanges. According to the literature research results, this is the first report and molecular analysis of Alfalfa mosaic virus (AMV) in Medicago sativa L. in Van province of Turkey.

Anahtar Kelimeler

Alfalfa RT-PCR characterisation sequencing phylogenetic analysis

Kaynakça

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  • Alhudaib KA, 2019. Serological and Molecular Detection of Alfalfa mosaic virus in the Major Potato Growing Areas of Saudi Arabia. Scientifc Journal of King Faisal University (Basic and Applied Sciences), 20 (1): 73- 82.
  • Ali H, Gholam K, 2011. Detection and Identification of Clavibacter michiganensis subsp. insidiosus inducing Alfalfa Wilt Disease Using Specific PCR and Physiological Methods in Iran. Research Journal of Chemistry and Environment, 15 (2): 486-491.
  • Al-Saleh MA, Amer MA, 2013. Biological and Molecular variability of Alfalfa mosaic virus affecting alfalfa crop in riyadh region. The Plant Pathology Journal, 29(4) : 410-417.
  • Al-Shahwan IM, Abdalla OA, Al-Saleh MA, Amer MA, 2017. Detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in Saudi Arabia. Saudi Journal of Biological Sciences, 24 (6): 1336- 1343.
  • Anonim, 2014. T. C. Gıda Tarım ve Hayvancılık Bakanlığı, Tarla Bitkileri Araştırmaları Dairesi Başkanlığı, Çayır Mera ve Yem Bitkileri Araştırmaları Çalışma Grubu Raporu. https://www.tarimorman.gov.tr/TAGEM/Belgeler/SUNULAR/%C3%87MYB_Birg%C3%B Cl%20G%C3%BCner.PDF (Erişim Tarihi: 26.12.2018).
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Van İlinde Yoncada Saptanan Yonca mozaik virüs (AMV) İzolatlarının Kılıf Protein Genomunun Moleküler Karakterizasyonu

Yıl 2020, Cilt: 10 Sayı: 4, 2366 - 2377, 15.12.2020

Mustafa Usta , Abdullah Güller

https://doi.org/10.21597/jist.719099
Cited By: 3

Öz

Van ilinde yetiştiriciliği yapılan yonca (Medicago sativa L.) bitkilerinde 2019 yılında virüslerin oluşturduğu simptomlara benzer belirtiler görülmüştür. Gözlenen belirtiler arasında cüceleşme, yapraklarda mozaik deseni ve sararma, rozetlenme ve küçük yaprak oluşumu yer almaktadır. Simptom gösteren ve göstermeyen bitkilerden toplanan 19 yonca yaprak örneğine, virüs genomunu tespit edebilmek için uygun primer çiftleri ile RT-PCR testi uygulanmıştır. Yonca yaprağı örneklerinden şiddetli belirtiye sahip altısı beklenen büyüklükte 700 bp DNA band oluşturmuştur. Bunlar arasından rastgele seçilen ikisi bir plazmid vektörüne klonlanmıştır. Elde edilen rekombinant plazmidler her iki yönde dizilenmiştir. Dizi analizi sonuçlarına göre infekteli yoncalardaki virüsün Yonca mozaik virüs olduğu açığa çıkarılmıştır. Dizi bilgileri MT210179 ve MT210178 erişim numaraları ile gen bankasına yüklenmiştir ve sırasıyla Alakoy Y9 ve Alakoy Y1 olarak isimlendirilmiştir. Her iki dizi için gen bankasına kayıtlı 16 AMV dizisi ile oluşturulan filogenetik ağaca göre, her iki izolat da nükleotit düzeyinde ABD, Brezilya ve Puglia izolatları ile en yüksek benzerlik ve Güney Kore izolatı ile en düşük benzerlik oranı göstermiştir. Ayrıca her iki dizinin birbirleri arasında, 7 nükleotit değişikliği ile %98.45 oranında nükleotit benzerliği gösterdiği ortaya çıkarılmıştır. Gerçekleştirilen litaratür tarama çalışmalarına göre, bu çalışma Türkiye'nin Van ilinde yetiştirilen yonca bitkilerindeki Yonca mozaik virüsü (AMV)’ nün ilk raporu ve moleküler analizidir.

Anahtar Kelimeler

Dizileme filogenetik analiz RT-PCR karakterizasyon yonca

Kaynakça

  • Abdalla OA, Al-Shahwan IM, AL-Saleh MA, Amer MA, 2019. Molecular characterization of Alfalfa mosaic virus (AMV) isolates in alfalfa and other plant species in different regions in Saudi Arabia. European Journal of Plant Pathology,156: 603–613.
  • Alan B, Crabtree K, Dallwitz M, Gibbs A, Watson L, 1996. Viruses of Plants. Description and Lists from the VIDE Database. CAB International, UK,1484 PP.
  • Alfaro-Fernández A, Sánchez-Navarro J A, Landeira M, Font MI, Hernández-Llópis D, Pallás V, 2016. Evaluation of PCR and non-radioactive molecular hybridization techniques for the routine diagnosis of Tomato leaf curl new delhi virus, Tomato yellow leaf curl virus and Tomato yellow leaf curl sardinia virus. Journal of Plant Pathology, 98 (2): 245-254.
  • Alhudaib KA, 2019. Serological and Molecular Detection of Alfalfa mosaic virus in the Major Potato Growing Areas of Saudi Arabia. Scientifc Journal of King Faisal University (Basic and Applied Sciences), 20 (1): 73- 82.
  • Ali H, Gholam K, 2011. Detection and Identification of Clavibacter michiganensis subsp. insidiosus inducing Alfalfa Wilt Disease Using Specific PCR and Physiological Methods in Iran. Research Journal of Chemistry and Environment, 15 (2): 486-491.
  • Al-Saleh MA, Amer MA, 2013. Biological and Molecular variability of Alfalfa mosaic virus affecting alfalfa crop in riyadh region. The Plant Pathology Journal, 29(4) : 410-417.
  • Al-Shahwan IM, Abdalla OA, Al-Saleh MA, Amer MA, 2017. Detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in Saudi Arabia. Saudi Journal of Biological Sciences, 24 (6): 1336- 1343.
  • Anonim, 2014. T. C. Gıda Tarım ve Hayvancılık Bakanlığı, Tarla Bitkileri Araştırmaları Dairesi Başkanlığı, Çayır Mera ve Yem Bitkileri Araştırmaları Çalışma Grubu Raporu. https://www.tarimorman.gov.tr/TAGEM/Belgeler/SUNULAR/%C3%87MYB_Birg%C3%B Cl%20G%C3%BCner.PDF (Erişim Tarihi: 26.12.2018).
  • Anonymous 2017. Bugem Activities. T. C. Ministry of Food, Agriculture and Livestock, General Directorate of Plant Production, March 2017 (in Turkish).
  • Arlı-Sökmen M, Mennan H, Sevik MA, Ecevit O, 2005. Occurence of viruses in field-grown pepper crops and some of their reservoir weeds hosts in Samsun, Turkey. Phytoparasitica, 33(4), 347-358.
  • Bailiss KW, Ollennu LAA. 1986. Effect of Alfalfa mosaic virus isolates on forage yield of lucern (Medicago sativa) in Britain. Plant Pathology, 35: 162- 168.
  • Balasubramaniam M, Kim BS, Hutchens-Williams HM, Loesch-Fries LS, 2014. The photosystem II oxygen-evolving complex protein PsbP interacts with the coat protein of Alfalfa mosaic virus and inhibits virus replication. Molecular Plant-Microbe Interactions, 27(10): 1107- 1118.
  • Bol JF, 2003. Alfalfa mosaic virus: Coat protein dependent initiation of infection. Molecular Plant Pathology, 4(1): 1-8.
  • Brunt A, Crabtree K, Gibbs A, 1990. Viruses of Tropical Plants. CAB International, London.
  • Buzkan N, Demir M, Öztekin V, Mart C, Çağlar BK, Yilmaz MA, 2006. Evaluation of the status of capsicum viruses in the main growing regions of Turkey. Bulletin OEPP, 36(1): 15–19.
  • Chod J, Chodová D, Kočova M, Jokeš M, 1994. Occurrence of Alfalfa mosaic virus on red pepper (Capsicum annuum L.) and investigation of susceptibility of some cultivars with respect to Hill’s reaction activity and chlorophyll content. Zahradnictví, 21: 27–36.
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  • Özdemir S, Erilmez S, Paylan Cİ, 2011. Ege Bölgesi Patates Üretim Alanlarında Alfalfa mosaic alfamovirus’ün Serolojik ve Moleküler Olarak Tanılanması. Türkiye IV. Bitki Koruma Kongresi Bildirileri: 410.
  • Paliwal YC, 1982. Virus diseases of alfalfa and biology of Alfalfa mosaic virus in Ontario and western Quebec. Canadian Journal of Plant Pathology, 4: 175- 179.
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  • Peña E, Olate E, Chorbadjian RA, Rosales IM, 2011. First Report of Alfalfa mosaic virus Infection in Viburnum tinus in Chile. Plant Disease, 95(9):1198.
  • Peterson JJ, Samac DA, Grau CR, 2018. First Report of Fusarium Wilt of Alfalfa Caused by Fusarium oxysporum f. sp. medicaginis in Wisconsin. Disease Notes. 102 (2): 447.
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  • Qin F, Liu D, Sun B, Ruan L, Ma Z, Wang H, 2016. Identification of Alfalfa Leaf Diseases Using Image Recognition Technology. PLoS ONE 11(12): e0168274. doi:10.1371/journal. Pone.0168274.
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  • Zitikaitė I, Samuitienė M, 2008. Identifcation and some properties of Alfalfa mosaic alfamovirus isolated from naturally infected tomato cropbiologija. Biologija. 54 (2): 83–88.
Toplam 87 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Agronomi
Bölüm Bitki Koruma / Plant Protection
Yazarlar

Mustafa Usta 0000-0002-3940-2774

Abdullah Güller 0000-0003-3887-4208

Yayımlanma Tarihi 15 Aralık 2020
Gönderilme Tarihi 13 Nisan 2020
Kabul Tarihi 4 Temmuz 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 10 Sayı: 4

Kaynak Göster

APA Usta, M., & Güller, A. (2020). Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Journal of the Institute of Science and Technology, 10(4), 2366-2377. https://doi.org/10.21597/jist.719099
AMA Usta M, Güller A. Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Iğdır Üniv. Fen Bil Enst. Der. Aralık 2020;10(4):2366-2377. doi:10.21597/jist.719099
Chicago Usta, Mustafa, ve Abdullah Güller. “Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province”. Journal of the Institute of Science and Technology 10, sy. 4 (Aralık 2020): 2366-77. https://doi.org/10.21597/jist.719099.
EndNote Usta M, Güller A (01 Aralık 2020) Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Journal of the Institute of Science and Technology 10 4 2366–2377.
IEEE M. Usta ve A. Güller, “Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province”, Iğdır Üniv. Fen Bil Enst. Der., c. 10, sy. 4, ss. 2366–2377, 2020, doi: 10.21597/jist.719099.
ISNAD Usta, Mustafa - Güller, Abdullah. “Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province”. Journal of the Institute of Science and Technology 10/4 (Aralık 2020), 2366-2377. https://doi.org/10.21597/jist.719099.
JAMA Usta M, Güller A. Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Iğdır Üniv. Fen Bil Enst. Der. 2020;10:2366–2377.
MLA Usta, Mustafa ve Abdullah Güller. “Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province”. Journal of the Institute of Science and Technology, c. 10, sy. 4, 2020, ss. 2366-77, doi:10.21597/jist.719099.
Vancouver Usta M, Güller A. Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Iğdır Üniv. Fen Bil Enst. Der. 2020;10(4):2366-77.

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