Micromonospora ve Nonomuraea İzolatlarının Moleküler Tanımlamaları ve Antimikrobiyal Aktivitelerinin Belirlenmesi

Yıl 2022, Cilt: 12 Sayı: 4, 2004 - 2013, 01.12.2022

Fadime Özdemir Koçak

https://doi.org/10.21597/jist.1146165

Öz

Aktinobakteriler farklı ekosistemlerde yaşayabilen ve sekonder metabolit açısından zengin kaynak oluşturan
bakteri grubunun en büyük üyesini oluşturmaktadır. Nadir aktinobakteriler ise aktif metabolitler açısından önemli
hedeflerden biri haline gelmiştir. Nadir aktinobakterilerden olan Micromonospora ve Nonomuraea son dönemde en çok ilgi
çeken cinslerdendir. Sunulan çalışmada endemik bir türden Micromonospora ve Nonomuraea cins üyelerinin izolasyonu
hedeflenmiştir. İzolatların moleküler tanımlamalarında 16S rRNA gen bölgesi analizleri kullanılmıştır. Analizler
sonucunda 10 izolatın Micromonospora üyesi, 2 izolatın Nonomuraea üyesi olduğu belirlenmiştir. Nonomuraea sp. HCI 01
ve HCI 02 suşları Micromonospora sp. HCI 04, HCI 23, HCI 39, HCI 44 ve HCI 49 suşlarının olası yeni birer tür olma
potansiyeline sahip olduğu tespit edilmiştir. Antimikrobiyal aktivite çalışmalarında Micromonospora sp. HCI 45, HCI 47
ve HCI 49 suşları E. coli’ye karşı, HCI 20 ve HSF 02 S. aureus’a karşı ve HCI 04 ve HSF 02 ise S. cerevisiae’a karşı
etkinlik gösterdiği belirlenmiştir. Nonomuraea sp. ise HCI 02 S. cerevisiae’a karşı antimikrobiyal aktiviteye sahip olduğu
görülmüştür. Elde edilen sonuçlarla, olası yeni türlerin olduğu ve antibiyotik olma potansiyeline sahip biyolojik aktif
moleküllerin varlığı gösterilmiştir.

Anahtar Kelimeler

16S rRNA, antimikrobiyal aktivite, Micromonospora, Nonomuraea

Destekleyen Kurum

Bilecik Şeyh Edebali Universitesi

Proje Numarası

2013-01.BİL.13-01

Teşekkür

Bilecik Şeyh Edebali Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğüne teşekkür ederim.

Molecular Identification and Determination of Antimicrobial Activity of Micromonospora and Nonomuraea Isolates

Öz

Actinobacteria are the largest member of the Bacteria group, which can live in different ecosystems and
create a rich source of secondary metabolites. Rare actinobacteria have become one of the important targets in terms of
active metabolites. Micromonospora and Nonomuraea, which are rare Actinobacteria, are among the genera that have
attracted the most attention recently. In the present study, it was aimed to isolate Micromonospora and Nonomuraea genus
members from an endemic species. 16S rRNA gene region analyzes were used for molecular identification of isolates. As a
result of the analysis, it was determined that 10 isolates were members of Micromonospora and 2 isolates were members of
Nonomuraea. It has been determined that Nonomuraea sp. HCI 01 and HCI 02 strains and Micromonospora sp. HCI 04,
HCI 23, HCI 39, HCI 44 and HCI 49 strains have the potential to become a possible new species. In biological activity
studies, Micromonospora sp. HCI 45, HCI 47 and HCI 49 strains were found to be active against E. coli, HCI 20 and HSF
02 against S aureus, and HCI 04 and HSF 02 against S. cerevisiae. Nonomuraea sp. HCl 02 was found to have
antimicrobial activity against S. cerevisiae. The results obtained have shown that there are possible new species and the
existence of biologically active molecules with the potential to be antibiotics.

Anahtar Kelimeler

16S rRNA, antimicrobial activity, Micromonospora, Nonomuraea

Proje Numarası

2013-01.BİL.13-01

Kaynakça

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