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Comparative Study of Antagonistic Activity of Scots Pine Root Associated Mycorrhizal Fungus-Bacteria and Wheat Associated Bacteria Against Plant Pathogenic Fungi

Yıl 2011, Cilt: 1 Sayı: 2, 57 - 60, 30.06.2011

Öz

The aim of the present work was to test antagonistic activity of Scots pine root associated mycorrhizal

fungus-bacteria and wheat associated bacteria against pathogenic fungi Fusarium culmorum, Rhizoctonia

solani, Botrytis cinera and to determine the production of extracellular lytic enzymes by bacterial strains. The

higher number of bacterial strains isolated from wheat showed antagonistic activity compared to bacterial strains

isolated from mycorrhizal hyphae. The best performing strains from mycorrhizal hyphae were Arthrobacter ilicis

KNCL24, Rhodococcus. fascines HNOL8 and from wheat Bacillus cohnii 19, B. subtilis 1, and B. lentus 28. All

isolates presented high level of pectinase activity. Only four strains A. ilicis KNCL24, B. lentus 17, B. subtilis 4, B.

halodurans 12 were able to produce hydrogen cyanide (HCN). Our results showed that bacterial strains associated

with wheat possess more antagonistic activity compared to bacterial strains from mycorrhizal hyphae. The all tested

bacterial strains produced one or more cell wall degrading enzymes.

Kaynakça

  • Abd Rahman R.N.Z., Geok L., Basri M., Abd Rahmen B.S., 2005. Physical factors affecting the production of organic solvent- tolerant protease by Pseudomonas aeruginosa strain K . Bio resource Technology 96: 429-436.
  • Adams, P.B., 1990. The potential of mycoparasites for biological control of plant diseases. Annual Review of Phytopathology 28: 59–72.
  • Barea, J.M., Azcon R., Azcon-Aguilar C., 2002. Mycorrhizosphere interactions to improve plant fitness and soil quality. Antonie Van Leeuwenhoek 81: 343–351.
  • Berg, G., Krechel A., Ditz, M., Sikora, R., Ulrich, A., Hallmann, J., 2005. Endophytic and ectophytic potato-associated bacte- rial communities differ in structure and antagonistic function against plant pathogenic fungi. FEMS Microbiology Ecology 51: 215-229.
  • Bergsma-Vlami, M., Prins, M.E., Staatzs, M., Raaijmakers, J.M., 2005. Assessment of genotypic diversity of antibiotic produc- ing Pseudomonas species in the rhizosphere by denaturing gradient gel electrophoresis. Applied and Environmental Mi- crobiology 71: 993-1003.
  • Castric, P.A., 1975. Hydrogen cyanide, a secondary metabolite of Pseudomonas aeruginosa. Can J Microbiol 21: 613–618.
  • Chin-A-Woeng, T.F.C., Thomas-Oates, J.E., Lugtenberg B.J.J., Bloemberg, G.V., 2001. Introduction of the phzH gene of Pseudomonas chlororaphis PCL1391 extends the range of biocontrol ability of phenazine-1-carboxylic acid-producing Pseudomonas spp. strains. Molecular Plant–Microbe Interac- tions 14: 1006–1015.
  • Cook, R.J., Thomashow, L.S., Weller, D.M., Fujimoto, D., Maz- zola, M., Banger, G., Kim, D.S., 1995. Molecular mechanisms of defense by rhizobacteria against root diseases. Proceedings of National Academy of Sciences, USA 92: 4197-4201.
  • De Boer, M., Bom, P., Kindt, F., Keurentjes, J.J.B., Van Der Sluis, I., Van Loon, L.C., Bakker, P.A.H.M. 2003. Control of Fusarium wilt of radish by combining Pseudomonas putida strains that have different disease-suppressive mechanisms. Phytopathol- ogy 93: 626-632.
  • Egamberdieva, D., Kucharova, Z., Davranov, K., Berg, G., Makaro- va, N., Azarova, T., Chebotar, V., Tikhonovich, I., Kamilova, F., Validov, Sh. Lugtenberg, B., 2010. Bacteria able to control foot and root rot and to promote growth of cucumber in sali- nated soils. Biology Fertility of Soils, 47:197-205.
  • Egamberdiyeva, D., Hoflich, G., 2002. Root colonization and growth promotion of winter wheat and pea by Cellulomonas spp. at different temperatures. Journal of Plant Growth Regu- lation 38: 219-224.
  • Egamberdiyeva, D., Hoflich, G., 2003. The effect of associative bacteria from different climates on plant growth of pea at dif- ferent soils and temperatures. Archive Agronomy and Soil Science 49: 203-213.
  • Gamalero, E., Martinotti, M.G., Trotta, A., Lemanceau, P., Berta, G., 2004. Morphogenetic modifications induced by Pseudomo- nas fluorescens A6RI and Glomus mosseae BEG12 in the root system of tomato differ according to plant growth conditions. New Phytol 155: 293–300.
  • Garbaye, J., 1994. Helper bacteria: a new dimension to the mycor- rhizal symbiosis. New Phytol 128: 197–210.
  • Haas, D., Défago, G., 2005. Biological control of soil-borne patho- gens by fluorescent pseudomonads. Nature Reviews Microbi- ology 3: 307-319.
  • Howe, T.G., Ward, J.M., 1976. The utilization of Tween 80 as car- bon source by Pseudomonas. J Gen Microbiol 92: 234-235.
  • Höflich, G., Wiehe, W., Kühn, G. 1994. Plant growth stimulation with symbiotic and associative rhizosphere microorganisms. Experientia 50: 897-905.
  • Jiang, Y.M., Zhu, X.R., Li, Y.B., 2001. Postharvest control of litchi fruit rot by Bacillus subtilis. Lebensm. Wiss. U. Technol., 34: 430-436.
  • Lugtenberg, B.J.J, Chin-A-Woeng, T. F.C., Bleomberg, G.V., 2002. Microbe-plant interactions: principles and mechanisms. An- tonie van Leeuwenhoek 81: 373-383.
  • Loper, J.E., Henkels, M.D., 1997. Availability of iron to Pseudomo- nas fluorescens in rhizosphere and bulk soil evaluated with an ice nucleation reporter gene. Applied and Environmental Microbiology 63: 99–105.
  • Nielson, M.N., Sorensen, J., 1999. Chitinolytic activity of Pseudomonas fluorescens isolates from barley and sugar beet rhizosphere. FEMS Microbiology, Ecology 30: 217–227.
  • Raaijmakers, J.M., Vlami, M., De Souza, J.T., 2002. Antibiotic pro- duction by bacterial biocontrol agents. Antonie van Leeuwen- hoek 81: 537–547.
  • Smibert, R.M., Krieg, N.R., 1994. Phenotypic characterization. In: Methods for General and Molecular Bacteriology eds. P. Ger- hardt et al. Washington, D.C.: American Society for Microbi- ology. pp. 607-654.
  • Walsh, G.A., Murphy, R.A., Killeen, G.F., Headon, D.R., Power, R.F., 1995. Technical note: Detection and quantification of supplemental fungal b-glucanase activity in animal feed. J Anim Sci 73: 1074-1076.

İskoçya Çam Köküyle İlişkili Mikorizal Mantar-Bakteriler ile Buğdayla İlişkili Bakterilerin Patojenik Mantarlara Karşı Antagonistik Etkilerinin Karşılaştırılması

Yıl 2011, Cilt: 1 Sayı: 2, 57 - 60, 30.06.2011

Öz

Bu çalışmanın amacı, İskoçya çam köküyle ilişkili mikorizal mantar bakterileri ile Fusarium culmorum, Rhizoctonia solani, Botrytis cinera gibi patojenik mantarlara karşı buğdayla ilişkili bakterilerin antagonistik aktivitesini test etmek, ve bakteri suşları ile ekstrasellüler litik enzimlerinin üretimini belirlemektir. Mikorizal hiflerden izole edilen bakteriyel suşlara nazaran, buğdaydan izole edilen yüksek sayıdaki bakteriyel suşlar antagonistik aktivite göstermiştir. Mikorizal hiflerden elde edilen en iyi performansa sahip suşların, Arthrobacter ilicis KNCL24, Rhodococcus. fascines HNOL8 ve buğdaylardan ise Bacillus cohnii 19, B. subtilis 1 ve B. lentus 28 olduğu belirlenmiştir. Tüm izolatlar, yüksek düzeyde pektinaz aktivitesi göstermiştir. Sadece 4 suş (A. ilicis KNCL24, B. lentus 17, B. subtilis 4, B. halodurans 12) hidrojen siyanür üretebilmiştir. Sonuçlarımız, buğdayla ilişkili bakteriyel suşların, mikorizal hiflerden elde edilen bakteriyel suşlardan daha fazla antagonistik aktiviteye sahip olduğunu göstermiştir. Test edilen tüm bakteriyel suşlar, bir yada daha fazla hücre duvarını parçalayan enzimleri üretmiştir

Kaynakça

  • Abd Rahman R.N.Z., Geok L., Basri M., Abd Rahmen B.S., 2005. Physical factors affecting the production of organic solvent- tolerant protease by Pseudomonas aeruginosa strain K . Bio resource Technology 96: 429-436.
  • Adams, P.B., 1990. The potential of mycoparasites for biological control of plant diseases. Annual Review of Phytopathology 28: 59–72.
  • Barea, J.M., Azcon R., Azcon-Aguilar C., 2002. Mycorrhizosphere interactions to improve plant fitness and soil quality. Antonie Van Leeuwenhoek 81: 343–351.
  • Berg, G., Krechel A., Ditz, M., Sikora, R., Ulrich, A., Hallmann, J., 2005. Endophytic and ectophytic potato-associated bacte- rial communities differ in structure and antagonistic function against plant pathogenic fungi. FEMS Microbiology Ecology 51: 215-229.
  • Bergsma-Vlami, M., Prins, M.E., Staatzs, M., Raaijmakers, J.M., 2005. Assessment of genotypic diversity of antibiotic produc- ing Pseudomonas species in the rhizosphere by denaturing gradient gel electrophoresis. Applied and Environmental Mi- crobiology 71: 993-1003.
  • Castric, P.A., 1975. Hydrogen cyanide, a secondary metabolite of Pseudomonas aeruginosa. Can J Microbiol 21: 613–618.
  • Chin-A-Woeng, T.F.C., Thomas-Oates, J.E., Lugtenberg B.J.J., Bloemberg, G.V., 2001. Introduction of the phzH gene of Pseudomonas chlororaphis PCL1391 extends the range of biocontrol ability of phenazine-1-carboxylic acid-producing Pseudomonas spp. strains. Molecular Plant–Microbe Interac- tions 14: 1006–1015.
  • Cook, R.J., Thomashow, L.S., Weller, D.M., Fujimoto, D., Maz- zola, M., Banger, G., Kim, D.S., 1995. Molecular mechanisms of defense by rhizobacteria against root diseases. Proceedings of National Academy of Sciences, USA 92: 4197-4201.
  • De Boer, M., Bom, P., Kindt, F., Keurentjes, J.J.B., Van Der Sluis, I., Van Loon, L.C., Bakker, P.A.H.M. 2003. Control of Fusarium wilt of radish by combining Pseudomonas putida strains that have different disease-suppressive mechanisms. Phytopathol- ogy 93: 626-632.
  • Egamberdieva, D., Kucharova, Z., Davranov, K., Berg, G., Makaro- va, N., Azarova, T., Chebotar, V., Tikhonovich, I., Kamilova, F., Validov, Sh. Lugtenberg, B., 2010. Bacteria able to control foot and root rot and to promote growth of cucumber in sali- nated soils. Biology Fertility of Soils, 47:197-205.
  • Egamberdiyeva, D., Hoflich, G., 2002. Root colonization and growth promotion of winter wheat and pea by Cellulomonas spp. at different temperatures. Journal of Plant Growth Regu- lation 38: 219-224.
  • Egamberdiyeva, D., Hoflich, G., 2003. The effect of associative bacteria from different climates on plant growth of pea at dif- ferent soils and temperatures. Archive Agronomy and Soil Science 49: 203-213.
  • Gamalero, E., Martinotti, M.G., Trotta, A., Lemanceau, P., Berta, G., 2004. Morphogenetic modifications induced by Pseudomo- nas fluorescens A6RI and Glomus mosseae BEG12 in the root system of tomato differ according to plant growth conditions. New Phytol 155: 293–300.
  • Garbaye, J., 1994. Helper bacteria: a new dimension to the mycor- rhizal symbiosis. New Phytol 128: 197–210.
  • Haas, D., Défago, G., 2005. Biological control of soil-borne patho- gens by fluorescent pseudomonads. Nature Reviews Microbi- ology 3: 307-319.
  • Howe, T.G., Ward, J.M., 1976. The utilization of Tween 80 as car- bon source by Pseudomonas. J Gen Microbiol 92: 234-235.
  • Höflich, G., Wiehe, W., Kühn, G. 1994. Plant growth stimulation with symbiotic and associative rhizosphere microorganisms. Experientia 50: 897-905.
  • Jiang, Y.M., Zhu, X.R., Li, Y.B., 2001. Postharvest control of litchi fruit rot by Bacillus subtilis. Lebensm. Wiss. U. Technol., 34: 430-436.
  • Lugtenberg, B.J.J, Chin-A-Woeng, T. F.C., Bleomberg, G.V., 2002. Microbe-plant interactions: principles and mechanisms. An- tonie van Leeuwenhoek 81: 373-383.
  • Loper, J.E., Henkels, M.D., 1997. Availability of iron to Pseudomo- nas fluorescens in rhizosphere and bulk soil evaluated with an ice nucleation reporter gene. Applied and Environmental Microbiology 63: 99–105.
  • Nielson, M.N., Sorensen, J., 1999. Chitinolytic activity of Pseudomonas fluorescens isolates from barley and sugar beet rhizosphere. FEMS Microbiology, Ecology 30: 217–227.
  • Raaijmakers, J.M., Vlami, M., De Souza, J.T., 2002. Antibiotic pro- duction by bacterial biocontrol agents. Antonie van Leeuwen- hoek 81: 537–547.
  • Smibert, R.M., Krieg, N.R., 1994. Phenotypic characterization. In: Methods for General and Molecular Bacteriology eds. P. Ger- hardt et al. Washington, D.C.: American Society for Microbi- ology. pp. 607-654.
  • Walsh, G.A., Murphy, R.A., Killeen, G.F., Headon, D.R., Power, R.F., 1995. Technical note: Detection and quantification of supplemental fungal b-glucanase activity in animal feed. J Anim Sci 73: 1074-1076.
Toplam 24 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Mühendislik
Bölüm Bitki Koruma / Plant Protection
Yazarlar

Egamberdieva Dılfuza Bu kişi benim

Yayımlanma Tarihi 30 Haziran 2011
Gönderilme Tarihi 26 Ocak 2011
Kabul Tarihi 25 Mayıs 2011
Yayımlandığı Sayı Yıl 2011 Cilt: 1 Sayı: 2

Kaynak Göster

APA Dılfuza, E. (2011). İskoçya Çam Köküyle İlişkili Mikorizal Mantar-Bakteriler ile Buğdayla İlişkili Bakterilerin Patojenik Mantarlara Karşı Antagonistik Etkilerinin Karşılaştırılması. Journal of the Institute of Science and Technology, 1(2), 57-60.
AMA Dılfuza E. İskoçya Çam Köküyle İlişkili Mikorizal Mantar-Bakteriler ile Buğdayla İlişkili Bakterilerin Patojenik Mantarlara Karşı Antagonistik Etkilerinin Karşılaştırılması. Iğdır Üniv. Fen Bil Enst. Der. Haziran 2011;1(2):57-60.
Chicago Dılfuza, Egamberdieva. “İskoçya Çam Köküyle İlişkili Mikorizal Mantar-Bakteriler Ile Buğdayla İlişkili Bakterilerin Patojenik Mantarlara Karşı Antagonistik Etkilerinin Karşılaştırılması”. Journal of the Institute of Science and Technology 1, sy. 2 (Haziran 2011): 57-60.
EndNote Dılfuza E (01 Haziran 2011) İskoçya Çam Köküyle İlişkili Mikorizal Mantar-Bakteriler ile Buğdayla İlişkili Bakterilerin Patojenik Mantarlara Karşı Antagonistik Etkilerinin Karşılaştırılması. Journal of the Institute of Science and Technology 1 2 57–60.
IEEE E. Dılfuza, “İskoçya Çam Köküyle İlişkili Mikorizal Mantar-Bakteriler ile Buğdayla İlişkili Bakterilerin Patojenik Mantarlara Karşı Antagonistik Etkilerinin Karşılaştırılması”, Iğdır Üniv. Fen Bil Enst. Der., c. 1, sy. 2, ss. 57–60, 2011.
ISNAD Dılfuza, Egamberdieva. “İskoçya Çam Köküyle İlişkili Mikorizal Mantar-Bakteriler Ile Buğdayla İlişkili Bakterilerin Patojenik Mantarlara Karşı Antagonistik Etkilerinin Karşılaştırılması”. Journal of the Institute of Science and Technology 1/2 (Haziran 2011), 57-60.
JAMA Dılfuza E. İskoçya Çam Köküyle İlişkili Mikorizal Mantar-Bakteriler ile Buğdayla İlişkili Bakterilerin Patojenik Mantarlara Karşı Antagonistik Etkilerinin Karşılaştırılması. Iğdır Üniv. Fen Bil Enst. Der. 2011;1:57–60.
MLA Dılfuza, Egamberdieva. “İskoçya Çam Köküyle İlişkili Mikorizal Mantar-Bakteriler Ile Buğdayla İlişkili Bakterilerin Patojenik Mantarlara Karşı Antagonistik Etkilerinin Karşılaştırılması”. Journal of the Institute of Science and Technology, c. 1, sy. 2, 2011, ss. 57-60.
Vancouver Dılfuza E. İskoçya Çam Köküyle İlişkili Mikorizal Mantar-Bakteriler ile Buğdayla İlişkili Bakterilerin Patojenik Mantarlara Karşı Antagonistik Etkilerinin Karşılaştırılması. Iğdır Üniv. Fen Bil Enst. Der. 2011;1(2):57-60.