Effects of Some Treatments Prior to Strati cation on Germination in Kalecik Karası (Vitis vinifera L.) Seeds

Yıl 2013, Cilt: 3 Sayı: 4, 9 - 13, 31.12.2013

Murat Akkurt Nurhan Keskı̇n , Mina Shidfar1, Atilla Çakir Shıdfar Atilla Çakır

Öz

Kalecik Karası cv. (Vitis vinifera L.) is one of the main grapevine cultivars in Turkey that is com- monly used in breeding programs. However, low germination of seeds is crucial problem for breeding programs. In order to increase the germination ratio of Kalecik Karası cv. seeds, they were strati ed at +4°C for 60 and 90 days following some pre-treatments with growth regulators such as benzylaminopurine (BAP), gibberellic acid (GA3), BAP + GA3, and hydrogen peroxide (H2O2). Maximum germination ratio (66.67%) for 60-day strati cation was re- corded after pre-treatment with 1 g/l BAP + 3 g/l GA3. In addition, for 90-day strati cation, maximum germination ratio was also observed 64% after pre-treatment with 0.5 g/l BAP + 2 g/l GA3. According to the results, the highest germination ratio in Kalecik Karası cv. seeds was realized in 0.5-1 g/L BAP + 2-3 g/L GA3 treatments.

Anahtar Kelimeler

Benzylaminopurine, gibberellic acid, hydrogen peroxide, strati cation, germination

Kalecik Karası (Vitis vinifera L.) Tohumlarında Katlama Öncesi Bazı Uygulamaların Çimlenme Üzerine Etkisi

Öz

Kalecik Karası, (Vitis Vinifera L.) Türkiye’de ıslah programlarında yaygın olarak kullanılan üzüm çeşit-
lerinden birisidir. Ancak, tohumlardaki düşük çimlenme oranı, ıslah çalışmalarında önemli bir sorundur. Kalecik
Karası tohumlarında çimlenme oranını artırmak için tohumlar; benzilaminopürin (BAP), Gibberellik asit (GA3),
BAP+GA3 ve Hidrojen peroksit (H2O2) gibi büyümeyi düzenleyici maddeler ile ön muamele yapıldıktan sonra
+4°C de 60 ve 90 gün süreyle katlamaya tabi tutulmuştur. En yüksek çimlenme oranı (%66.67) 1 g/l BAP + 3 g/l
GA3 uygulaması ve 60 gün katlama süresinde elde edilmiştir. Bunun yanı sıra, 90 gün katlama süresinde ise en
yüksek çimlenme oranı %64 ile 0.5 g/l BAP+2 g/l GA3 uygulamasında elde edilmiştir. Bu sonuçlara göre, Kalecik
Karası tohumlarında en yüksek çimlenme oranı 0.5-1 g/l BAP + 2-3 g/l GA3 uygulamalarında gerçekleşmiştir.

Anahtar Kelimeler

Benzilaminopürin, giberalik asit, hidrojen peroksit, katlama, çimlenme

Kaynakça

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