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Effect of lead of Some Morphological and Biochmical Properties in Crisp Lettuce Plants (Lactuca sativa var. crispa)

Yıl 2015, Cilt: 5 Sayı: 1, 83 - 88, 31.03.2015

Öz

In this study, effect of lead stress on lettuce plants was determined to some morphological and biochemical properties. The study was carried out under greenhouse conditions, after 30 days from seed to plants, it was applied three different lead concentration (0, 150, 300 ppm) with irrigation water. The plants were irrigated eld capacity level. After the four weeks, samples of plant were harvested. Shoot fresh and root fresh weight, shoot dry and root dry weight, stem and root length, leaf area, the amount of malondialdehit (MDA), superoxide dismutase (SOD) and glutathione reductase (GR) enzyme activities were investigated on sample plant. There were decline to plant biomass, stem and root length, leaf area on increased of lead applications with irrigation water. SOD and GR activities were higher on the treatment of application 300 ppm Pb than other treatment. Antioxidative enzyme system is highly effective in protecting against lead stress in plant.

Kaynakça

  • Anonymous, 2013. Türkiye İstatistik Kurumu (http://tuikrapor.tuik. gov.tr/ reports).
  • Akıncı, İ.E., Akıncı, S., Yılmaz, K., 2010. Response of tomato (Solanum lycopersicum L.) to lead toxicity: Growth, element uptake, chlorophyll and water content. African Journal of Agricultural Research, 5 (6): 416-423.
  • Cakmak, I., Marschner, H., 1992. Magnesium def ciency and highlight intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiology, 98: 1222-1226.
  • Ghani, A., Shah, A.U., Akhtar, U., 2010. Effect of lead toxicity on growth, chlorophyll and lead (Pb) content of two varieties of maize (Zea mays L.). Pakistan Journal of Nutrition, 9(9): 887- 891.
  • Jiang, W., Liu, D., 2000. Effects of Pb2+ on root growth, cell division, and nucleolus of Zea mays L. Bull. Environ. Contam. Toxicol., 65: 786-793.
  • Kabata-Pendias, A., Pendias, H., 1984. Trace element in the soil and plants. CRC Press Florida.
  • Kıran, Y., Munzuroğlu, Ö., 2004. Mercimek (Lens culinaris Medik.) tohumlarının çimlenmesi ve de büyümesi üzerine kurşunun etkileri. F.Ü. Fen ve Mühendislik Dergisi, 16 (1): 1-9.
  • Kuşvuran, Ş., 2010. Kavunlarda Kuraklık ve Tuzluluğa Toleransın Fizyolojik Mekanizmaları Arasındaki Bağlantılar. Çukurova Üniversitesi Fen Bilimleri Enst., Doktora Tezi, 355s., Adana.
  • Liu, D. H., Jiang, W. S., Wang, W., Zhao, F. M., Lu, C., 1994. Effects of lead on root growth cell division and nucleolus of Allium cepa. Environ. Pollut., 86: 1-4.
  • LiuY,WangX,ZengG,QuD,GuJ,ZhouM,ChaiL.2007. Cadmium-induced oxidative stress and response of the ascorbate-glutathione cycle in Bechmeria nivea (L.) Gaud. Chemosphere, 69: 99-107.
  • Lutts, S., Kinet, J.M., Bouharmont, J., 1996. NaCl-induced senesence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Ann. Bot., 78: 389-398.
  • Miranda, M.G., Ilangovan, K., 1996. Uptake of lead by Lemna gibba L. in unce on spesi c growth rate and basic biochemical changes. Bull. Environ. Contam. Toxicol., 56: 1000-1007.
  • Mittler, R., 2002. Oxidative stress, antioxidants, and stress tolerance. Trends in Plant Science, 7: 405-410.
  • Morela, V.R.F., Capraru, G., Bara, I., Artenie, V., 2007. Lead acetate effect on superoxide dismutase activity in Lactuca sativa L., Mona and Syrena cultivars. Genetics and Molecular Biology, 8 (2): 115-118.
  • Prasad, K.V.S.K., Paradha, S.P., Sharmila, P., 1999. Concerted action of antioxidant enzymes and curtailed growth under zinc toxicity in Brassica juncea. Environ. Exp. Bot., 42: 1-10.
  • Sharma, P., Dubey, R.S., 2005. Lead toxicity in plants. Braz. J. Plant Physiol., 17(1): 35-52.
  • Verma, S., Dubey, R.S., 2003. Lead toxcitiy induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Sci., 164: 645-655.
  • Yong, X., Zhang, Z., Yang, Q., 2011. Effect of lead stress on growth characteristic and physiological indexes of Alternanthera philoxeroides. Agric. Sci. and Tech.,12(3): 347-349.
  • Xiong, Z. T., 1998. Lead uptake and effects on seed germination and plant growth in a Pb hyperaccumulator Brassica pekinensis (Rupr). Bull. Environ. Contam. Toxicol., 60: 285-291.
  • Zhou, D.X., Liu, Y.F., Liu, X.B., 2009. Effects of waterlogging stress on physiological and biochemical index in Alternant phiiloxeroides Hubei. Agricultural Sciences, 48(3): 585-587.
  • Cakmak, I., 1994. Activity of ascorbate-dependent H 0 scavenging 22 enzymes and leaf chlorosis are enhancend in magnesium and potassium de cient leaves, but not in phosphorus de cient leaves. Journal Experiment Botany, 45: 1259-1266.
  • Çolak, U., Doğan, M., 2011. Kurşun uygulamasının Triticum aestivum L. cv. Ceyhan 99’daki bazı zyolojik etkileri. Biyoloji Bilimleri Araştırma Dergisi, 4(2): 49-53.
  • Daşgan, H.Y., Koç, S., 2009. Evaluation of salt tolerance in common bean genotypes by ion regulation and searching for screening parameters. Journal of Food, Agriculture Environment, 7(2): 363-372.
  • Dinakar, N., Nagajyothi, P.C., Suresh, S., Udaykiran, Y., Damodharam, T., 2008. Phytotoxicity of cadmium on protein, proline and antioxidant enzyme activities in growing Arachis hypogaea L. seedlings. Journal of Environmental Science, 20: 199–206.
  • Fargašová, A., 1994. Effect of Pb, Cd, Hg, As and Cr on germination and root growth of Sinapis alba seeds. Bull. Environ. Contam. Toxicology, 52: 452-456.
  • Foyer, C.H., Lopez-Delgado, H., Dat, J.F., Scott, I.M., 1997. Hydrogen peroxide and glutathione-associated mechanisms of acclimatory stress tolerance and signaling. Physiologia Plantarum, 100: 241–254.
  • Freed, R., Einensmith, S.P., Guets, S., Reicosky, D., Smail, V.W., Wolberg, P., 1989. User’s guide to MSTAT-C, an analysis of agronomic research experiment. Michigan State University, USA.

Kurşunun Kıvırcık Salata (Lactuca sativa var. crispa) Bitkisinin Bazı Morfolojik ve Biyokimyasal Özelliklerine Etkisi

Yıl 2015, Cilt: 5 Sayı: 1, 83 - 88, 31.03.2015

Öz





Bu çalışmada, kıvırcık salata bitkisinin bazı morfolojik ve biyokimyasal özellikleri üzerine kurşun stresinin
etkisini ortaya koymak amaçlanmıştır. Kontrollü sera koşullarında yürütülen çalışmada tohum ekiminden itibaren
30 günlük bitkilere 3 farklı kurşun (0, 150, 300 ppm) dozu, sulama suyu ile birlikte verilmiştir. Bitkiler 4 hafta
boyunca tarla kapasitesi düzeyinde sulandıktan sonra bu sürenin sonunda hasat edilerek örnekler alınmıştır.
İncelenen parametreler; toprak üstü biyomas ve kök yaş ağırlığı, toprak üstü biyomas ve kök kuru ağırlığı, gövde ve
kök boyu, yaprak alanı, malondialdehit (MDA) miktarı, süperoksit dismutaz (SOD) ve glutatyon redüktaz (GR)
enzim aktiviteleri olarak belirlenmiştir. Kurşun uygulamaları doz artışına paralel olarak kıvırcık salata bitkisinin
biyomas, gövde ve kök boyu, yaprak alanı değerlerinde düşüşe neden olmuştur. 300 ppm Pb uygulamasındaki
SOD ve GR aktiviteleri, diğer uygulamalardan daha yüksek bulunmuştur. Antioksidatif enzim sisteminin salata
bitkisinde kurşun stresine karşı korunmada oldukça etkin olduğu gözlemlenmiştir.





Kaynakça

  • Anonymous, 2013. Türkiye İstatistik Kurumu (http://tuikrapor.tuik. gov.tr/ reports).
  • Akıncı, İ.E., Akıncı, S., Yılmaz, K., 2010. Response of tomato (Solanum lycopersicum L.) to lead toxicity: Growth, element uptake, chlorophyll and water content. African Journal of Agricultural Research, 5 (6): 416-423.
  • Cakmak, I., Marschner, H., 1992. Magnesium def ciency and highlight intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiology, 98: 1222-1226.
  • Ghani, A., Shah, A.U., Akhtar, U., 2010. Effect of lead toxicity on growth, chlorophyll and lead (Pb) content of two varieties of maize (Zea mays L.). Pakistan Journal of Nutrition, 9(9): 887- 891.
  • Jiang, W., Liu, D., 2000. Effects of Pb2+ on root growth, cell division, and nucleolus of Zea mays L. Bull. Environ. Contam. Toxicol., 65: 786-793.
  • Kabata-Pendias, A., Pendias, H., 1984. Trace element in the soil and plants. CRC Press Florida.
  • Kıran, Y., Munzuroğlu, Ö., 2004. Mercimek (Lens culinaris Medik.) tohumlarının çimlenmesi ve de büyümesi üzerine kurşunun etkileri. F.Ü. Fen ve Mühendislik Dergisi, 16 (1): 1-9.
  • Kuşvuran, Ş., 2010. Kavunlarda Kuraklık ve Tuzluluğa Toleransın Fizyolojik Mekanizmaları Arasındaki Bağlantılar. Çukurova Üniversitesi Fen Bilimleri Enst., Doktora Tezi, 355s., Adana.
  • Liu, D. H., Jiang, W. S., Wang, W., Zhao, F. M., Lu, C., 1994. Effects of lead on root growth cell division and nucleolus of Allium cepa. Environ. Pollut., 86: 1-4.
  • LiuY,WangX,ZengG,QuD,GuJ,ZhouM,ChaiL.2007. Cadmium-induced oxidative stress and response of the ascorbate-glutathione cycle in Bechmeria nivea (L.) Gaud. Chemosphere, 69: 99-107.
  • Lutts, S., Kinet, J.M., Bouharmont, J., 1996. NaCl-induced senesence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Ann. Bot., 78: 389-398.
  • Miranda, M.G., Ilangovan, K., 1996. Uptake of lead by Lemna gibba L. in unce on spesi c growth rate and basic biochemical changes. Bull. Environ. Contam. Toxicol., 56: 1000-1007.
  • Mittler, R., 2002. Oxidative stress, antioxidants, and stress tolerance. Trends in Plant Science, 7: 405-410.
  • Morela, V.R.F., Capraru, G., Bara, I., Artenie, V., 2007. Lead acetate effect on superoxide dismutase activity in Lactuca sativa L., Mona and Syrena cultivars. Genetics and Molecular Biology, 8 (2): 115-118.
  • Prasad, K.V.S.K., Paradha, S.P., Sharmila, P., 1999. Concerted action of antioxidant enzymes and curtailed growth under zinc toxicity in Brassica juncea. Environ. Exp. Bot., 42: 1-10.
  • Sharma, P., Dubey, R.S., 2005. Lead toxicity in plants. Braz. J. Plant Physiol., 17(1): 35-52.
  • Verma, S., Dubey, R.S., 2003. Lead toxcitiy induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Sci., 164: 645-655.
  • Yong, X., Zhang, Z., Yang, Q., 2011. Effect of lead stress on growth characteristic and physiological indexes of Alternanthera philoxeroides. Agric. Sci. and Tech.,12(3): 347-349.
  • Xiong, Z. T., 1998. Lead uptake and effects on seed germination and plant growth in a Pb hyperaccumulator Brassica pekinensis (Rupr). Bull. Environ. Contam. Toxicol., 60: 285-291.
  • Zhou, D.X., Liu, Y.F., Liu, X.B., 2009. Effects of waterlogging stress on physiological and biochemical index in Alternant phiiloxeroides Hubei. Agricultural Sciences, 48(3): 585-587.
  • Cakmak, I., 1994. Activity of ascorbate-dependent H 0 scavenging 22 enzymes and leaf chlorosis are enhancend in magnesium and potassium de cient leaves, but not in phosphorus de cient leaves. Journal Experiment Botany, 45: 1259-1266.
  • Çolak, U., Doğan, M., 2011. Kurşun uygulamasının Triticum aestivum L. cv. Ceyhan 99’daki bazı zyolojik etkileri. Biyoloji Bilimleri Araştırma Dergisi, 4(2): 49-53.
  • Daşgan, H.Y., Koç, S., 2009. Evaluation of salt tolerance in common bean genotypes by ion regulation and searching for screening parameters. Journal of Food, Agriculture Environment, 7(2): 363-372.
  • Dinakar, N., Nagajyothi, P.C., Suresh, S., Udaykiran, Y., Damodharam, T., 2008. Phytotoxicity of cadmium on protein, proline and antioxidant enzyme activities in growing Arachis hypogaea L. seedlings. Journal of Environmental Science, 20: 199–206.
  • Fargašová, A., 1994. Effect of Pb, Cd, Hg, As and Cr on germination and root growth of Sinapis alba seeds. Bull. Environ. Contam. Toxicology, 52: 452-456.
  • Foyer, C.H., Lopez-Delgado, H., Dat, J.F., Scott, I.M., 1997. Hydrogen peroxide and glutathione-associated mechanisms of acclimatory stress tolerance and signaling. Physiologia Plantarum, 100: 241–254.
  • Freed, R., Einensmith, S.P., Guets, S., Reicosky, D., Smail, V.W., Wolberg, P., 1989. User’s guide to MSTAT-C, an analysis of agronomic research experiment. Michigan State University, USA.
Toplam 27 adet kaynakça vardır.

Ayrıntılar

Bölüm Toprak Bilimi ve Bitki Besleme / Soil Science and Plant Nutrition
Yazarlar

Sevinç Kıran Bu kişi benim

Fatma Özkay Bu kişi benim

Şebnem Kuşvuran Bu kişi benim

Şebnem Ellı̇altıoğlu Bu kişi benim

Yayımlanma Tarihi 31 Mart 2015
Gönderilme Tarihi 26 Eylül 2014
Kabul Tarihi 25 Şubat 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 5 Sayı: 1

Kaynak Göster

APA Kıran, S., Özkay F., Kuşvuran S., Ellı̇altıoğlu S. (2015). Effect of lead of Some Morphological and Biochmical Properties in Crisp Lettuce Plants (Lactuca sativa var. crispa). Journal of the Institute of Science and Technology, 5(1), 83-88.
AMA Kıran S, Özkay F, Kuşvuran S, Ellı̇altıoğlu S. Effect of lead of Some Morphological and Biochmical Properties in Crisp Lettuce Plants (Lactuca sativa var. crispa). Iğdır Üniv. Fen Bil Enst. Der. Mart 2015;5(1):83-88.
Chicago Kıran Sevinç, Özkay Fatma, Kuşvuran Şebnem, ve Ellı̇altıoğlu Şebnem. “Effect of Lead of Some Morphological and Biochmical Properties in Crisp Lettuce Plants (Lactuca Sativa Var. Crispa)”. Journal of the Institute of Science and Technology 5, sy. 1 (Mart 2015): 83-88.
EndNote Kıran S, Özkay F, Kuşvuran S, Ellı̇altıoğlu S (01 Mart 2015) Effect of lead of Some Morphological and Biochmical Properties in Crisp Lettuce Plants (Lactuca sativa var. crispa). Journal of the Institute of Science and Technology 5 1 83–88.
IEEE S. Kıran, Özkay F., Kuşvuran S., ve Ellı̇altıoğlu S., “Effect of lead of Some Morphological and Biochmical Properties in Crisp Lettuce Plants (Lactuca sativa var. crispa)”, Iğdır Üniv. Fen Bil Enst. Der., c. 5, sy. 1, ss. 83–88, 2015.
ISNAD Kıran Sevinç vd. “Effect of Lead of Some Morphological and Biochmical Properties in Crisp Lettuce Plants (Lactuca Sativa Var. Crispa)”. Journal of the Institute of Science and Technology 5/1 (Mart 2015), 83-88.
JAMA Kıran S, Özkay F, Kuşvuran S, Ellı̇altıoğlu S. Effect of lead of Some Morphological and Biochmical Properties in Crisp Lettuce Plants (Lactuca sativa var. crispa). Iğdır Üniv. Fen Bil Enst. Der. 2015;5:83–88.
MLA Kıran Sevinç vd. “Effect of Lead of Some Morphological and Biochmical Properties in Crisp Lettuce Plants (Lactuca Sativa Var. Crispa)”. Journal of the Institute of Science and Technology, c. 5, sy. 1, 2015, ss. 83-88.
Vancouver Kıran S, Özkay F, Kuşvuran S, Ellı̇altıoğlu S. Effect of lead of Some Morphological and Biochmical Properties in Crisp Lettuce Plants (Lactuca sativa var. crispa). Iğdır Üniv. Fen Bil Enst. Der. 2015;5(1):83-8.