Fatty acids, Vitamins and Antioxidant Properties of Cladophora fracta var. intricata

Öz

In this study investigations of the fatty acids, vitamins and antioxidant properties of cultured (CFI) Cladophora fracta var. intricata. CFI was isolated from benthic habitats of the Tokat Yeşilırmak River (Tokat) and axenic cultured. According to the antioxidant analysis results, a dose-dependent activity was observed in CFI in terms of DPPH free radical scavenging activity, FRAP and TEAC reducing power, but it was not found to be significant as it remained above IC50 values. In CFI, C14:0 Myristic acid, C16:0 Palmitic acid and C16:1 Palmitoleic acid, C18: 1 N9C Oleic fatty acids and vitamins A, C, E were found to be high. According to antioxidant analysis results, CFI was found to be notable for the use of its fatty acid and vitamin values in food and pharmaceutical. Since it has low contamination properties, the algal taxon examined can be easily used in culture media.

Anahtar Kelimeler

• Cladophora fracta var. intricata
• Antioxidant
• Fatty acids
• Vitamins A-C-E

Cladophora fracta var. intricata’nın Yağ Asitleri, Vitamin ve Antioksidan Özellikleri

Öz

Bu çalışmada kültüre alınan (CFI) Cladophora fracta var. intricata'nın yağ asitleri, vitaminleri ve antioksidan özellikleri araştırılmıştır. CFI, Tokat Yeşilırmak nehrinin bentik habitatlarından izole edilmiş ve daha sora aksenik kültüre alınmıştır. Yapılan antioksidan analiz sonuçlarına göre CFI’da DPPH serbest radikal giderme aktivitesi, FRAP ve TEAC indirgeme gücü açısından doza bağlı bir aktivite görülmüş ancak IC50 değerlerinin üzerinde kaldığı için anlamlı bulunmamıştır. CFI'da C14:0 Miristik asit, C16:0 Palmitik asit ve C16:1 Palmitoleik asit, C18:1 N9C Oleik yağ asitleri ve A, C, E vitaminleri yüksek bulunmuştur. Antioksidan analiz sonuçlarına göre CFI'nin yağ asidi ve vitamin değerlerinin gıda ve ilaç sektöründe kullanımı açısından dikkat çekici olduğu tespit edilmiştir. İncelenen alg takson, düşük kontaminasyon özelliklerine sahip olduğundan kültür ortamlarında rahatlıkla kullanılabilir.

Anahtar Kelimeler

• Cladophora fracta var. İntricata
• Antioksidan
• Yağ asitleri
• Vitamin A-C-E

Kaynakça

1. Akköz, C., Arslan, D., Ünver, A., Özcan, M.M.B., & Yılmaz, B. (2009). Chemical Composition, Total Phenolic and Mineral Contents of Enteromorpha Intestınalıs (L.) Kütz. and Cladophora glomerata (L.) Kütz. Seaweeds. Journal of Food Biochemistry, 35, 513–523. https://doi.org/10.1111/j.1745-4514.2010.00399.x.
2. Andersen, R.A. (2005). Algal Cultiring Techniques. Elsevier Academic Press, Boston.
3. Annette, A., Ajoe, J.K., Tom, S., & Jawahar, L.M. (2019). Vitamin E and its anticancer effects. Critical Reviews in Food Science and Nutrition, 59, 2831-2838. https://doi.org/10.1080/10408398.2018.1474169.
4. Baytaşoğlu, H., & Başusta, N. (2014). Deniz Canlılarının Tıp ve Eczacılık Alanlarında Kullanılması. Yunus Araştırma Bülteni, 2, 71-80. https://doi.org/10.17693/yunusae.v15i21956.235767.
5. Burtis, C.A., & Ashwood, E.R. (1999). Tietz Textbook of Clinical Chemistry, Third Edition. W.B. Saunders Company Press, Philadelphia.
6. Elmastaş, M., & Gulcin, I. (2006). A study on the in vitro antioxidant activity of juniper (Juniperus communis L.) fruit extracts. Analytical Letters, 39, 47-65. https://doi.org/10.1080/00032710500423385.
7. Gökpınar, Ş., Koray, T., Akçiçek, E., Göksan, T., & Durmaz, Y. (2006). Algal Antioksidanlar. E.Ü. Su Ürünleri Dergisi, 23, 85-89.
8. Göksan, T., Durmaz, Y., & Gökpınar, Ş. (2003). Effects of Light Path Lengths and Initial Culture Density on The Cultivation of Chaetoceros Muelleri. Aquaculture, 217, 431-436.

9. Guiry, M.D., & Guiry, G.M. (2021). AlgaeBase. National University of Ireland, Galway. Available from http://www.algaebase.org (accessed: 20 July 2021).
10. Günal-Köroğlu, D., Süyünç, G., Yıldırım, R., &Turan, S. (2021). Antioxidant Potential of Some Fruit Extracts Prepared with Different Solvents. Journal of the Institute of Science and Technology, 11(2): 1127-1139. https://doi.org/10.21597/jist.75386.
11. Hoek, C.V.D. (1963). Revision of the European species of Cladophora. Brill Academic Publishers, Leiden.
12. Itoa, H., Kasamab, K., Narusec, S., & Shimurad, K. (1982). Antitumor effect of palmitoleic acid on Ehrlich ascites tumor. Cancer Letters, 17, 197-203.
13. Karan, T., & Erenler, R. (2018). Fatty acid constituents and anticancer activity of Cladophora fracta (OF Müller ex Vahl) Kützing. Tropical Journal of Pharmaceutical Research, October 17, 1977-1982. https://doi.org/10.4314/tjpr.v17i10.12.
14. Kartal, M., Orhan, I., Mahmud, A.A., Şenol, F.S., Atici, T., & Şener, B. (2009). Antioxidant and anticholinesterase assets and liquid chromatography-mass spectrometry preface of various fresh-water and marine macroalgae. Pharmacognosy Magazine, 5, 291-297. https://doi.org/10.4103/0973-1296.58147.
15. Krish, S., & Das, A. (2014). In-vitro bioactivity of marine seaweed, Cladophora rupestris. International Journal of Pharma and Bio Sciences, 5, 898-908. http://dx.doi.org/10.22376/ijpbs.2019.10.1.p1-12.
16. Lezcano, V., Fernández, C., Parodi, E.R., & Morelli, S. (2018). Antitumor and antioxidant activity of the f reshwater macroalga Cladophora surera. Journal of Applied Phycology, 1-9. http://doi.org/10.1007/s10811-018-1422-5.
17. Lobban, C.S., Chapman, D.J., & Kremer, B.P. (1988). Experimental Phycology A Laboratory Manual. Chambridge University Press, Chambridge.
18. Liyana-Pathirana, C.M., & Shahidi, F. (2005). Antioxidant activity of commercial soft and hard wheat (Triticum aestivum L.) as affected by gastric pH conditions. Journal of Agricultural and Food Chemistry, 6, 2433-40. https://doi.org/10.1021/jf049320i.
19. Lund, J.W.G. (2002). The Freshwater Algal Flora of the British Isles. Cambridge University Press, Chambridge.
20. Michalak, I., & Chojnacka, K. (2015). Algae as production systems of bioactive compounds. Engineering Life Science, 15, 160–176. https://doi.org/10.1002/elsc.201400191.
21. Moreno, P., & Salvadó, V. (2000). Determination of eight water- and fat-soluble vitamins in multi-vitamin pharmaceutical formulations by high-performance liquid chromatography. Journal of Chromatography A, 870, 207–215. https://doi.org/10.1016/s0021-9673(99)01021-3.
22. Nurjanah, A.A., Rahmadhani, A., & Seulalae, A.V. (2021). Antioxidant activity and combination characteristics of filtrates and Sargassum polycystum seaweed salt residue. Kuwait Journal of Sciences, 1-12.
23. Oyaizu, M. (1986). Studies on products of browing reaction-antioxidative activities of products browing reaction prepared from glucosamine. Japanese Journal of Nutrition, 44, 307-315.
24. Pabuçcu, K., Yılmaz, N., Şahin, F., Bayrak, Ö.F., Canpolat, E., & Demiriz Yücer, T. (2018). The Comparisons of Fatty Acid Composition in Some Marine and Freshwater Algae. Wulfenia Journal, 25, 40-47.
25. Padayatty, S.J., Katz, A., Wang, Y., Eck, P., Kwon, O., Lee, J.H., Chen, S., Corpe, C., Dutta, A., Dutta, S.K., & Levine, M. (2003). Vitamin C as an Antioxidant: Evaluation of Its Role in Disease Prevention. Journal of the American College of Nutrition, 22, 18-35. https://doi.org/10.1080/07315724.2003.10719272.
26. Paquot, C. (1979). Standarts methods for analysis of oils, fats and derivatives 6th edition. Oxford Pergamon Press, Oxford.
27. Prescott, G. W. (1970). Algae of The Western Great Lakes Area, WM.C. Brown Company Publishers, USA.
28. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rıce-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231–1237. https://doi.org/10.1016/s0891-5849(98)00315-3.
29. Rose, P.D., Brady, D., Letebele, B., & Duncan, J.R. (1994). Bioaccumulation of Metals by Scenedesmus, Selenastrum and Chlorella algae. Water SA, 20, 213-218.
30. Tanumihardjo, S.A. (2011). Vitamin A: biomarkers of nutrition for development. The American Journal of Clinical Nutrition, 94, 658-665. https://doi.org/10.3945/ajcn.110.005777.
31. Zbakh, H., Chiheb, I., Motilva, V., & Riadi, H. (2014). Antibacterial, cytotoxic and antioxidant potentials of Cladophora prolifera (Roth) Kutzing collected from the Mediterranean coast of Morocco. American Journal of Phytomedicine and Clinical Therapeutics, 2, 1187-1199.