Horozibiği (Amaranthus Albus L.) Bitkisinde Putresin Uygulamasının Bazı Fizyolojik ve Biyokimyasal Parametreler Üzerine Etkisi

Yıl 2025, Cilt: 15 Sayı: 1, 343 - 352, 01.03.2025

Tülay Toprak , Rüveyde Tunçtürk , Murat Tunçtürk

https://doi.org/10.21597/jist.1564573

Öz

Bu çalışma; insan gıdası, kaba yem ve yağı için yetiştirilen ve son yıllarda üzerinde yoğun araştırmalar yapılan horoz ibiği (Amaranthus albus L.) bitkisinde putresin uygulamalarının bazı fizyolojik parametreler üzerine etkilerini belirlemek amacıyla yürütülmüştür. Çalışma 2022 yılında Van YYÜ Ziraat Fakültesi Tarla Bitkileri Bölümü’ ne ait tam kontrollü iklim odasında Tesadüf Parselleri Deneme Deseni’ ne göre faktöriyel düzende kurulmuştur. Dört tekerrürlü olarak kurulan denemede horoz ibiği bitkisi ve 4 farklı putresin dozu (0, 50, 100 ve 150 mM) kullanılmıştır. Araştırmada, toplam antioksidan aktivite, toplam fenolik madde, toplam flavonoid madde, askorbik asit, toplam karotenoid, malonildialdehit (MDA), klorofil a, b ve toplam klorofil içeriği gibi parametreler incelenmiştir. Araştırma sonucunda; putresin dozlarının klorofil a ve toplam klorofil miktarları hariç incelenen diğer parametreler üzerindeki etkisi istatistiksel olarak önemli bulunmuştur. Çalışmada, en yüksek antioksidan aktivite miktarı (19.66 µmol TE/ gTA), toplam fenolik madde miktarı (108.0 mg GAE /gTA), toplam flavonoid madde miktarı (130.37 mg QE /100gTA), askorbik asit içeriği (22.58 mg LAA /100gTA) ve toplam karotenoid içeriği (5.35 μg/g TA) 50 mM putresin uygulamalarından, en yüksek MDA içeriği kontrol parsellerinden ve en yüksek klorofil b içeriği (13.76 μg/g TA) 100 mM putresin uygulamalarından tespit edilmiştir. En düşük değerler ise genel olarak kontrol parsellerinden belirlenmiştir. Putresin uygulamaları kontrole kıyasla incelenen parametreler üzerinde olumlu ve etkili olmuştur. Bitki üzerinde kullanılan dozların herhangi bir strese neden olmadığı, MDA miktarının, kontrole göre doz arttıkça azaldığı belirlenmiştir.

Anahtar Kelimeler

Biyokimyasal-madde miktarı, horoz ibiği, klorofil, putresin, tıbbi bitkiler

Effects of Putrescine Applications on Some Physiological and Biochemical Parameters in amaranth (Amaranthus Albus L.) plant

Öz

This study was conducted to determine the effects of putrescine applications on some physiological parameters in Amaranthus albus L., a plant that is grown for human food, fodder or oil and has been the subject of intensive research in recent years. The study was set up in a factorial design based on the Randomized Complete Block Design with four replications in a fully controlled climate chamber at the Department of Field Crops, Faculty of Agriculture, Van Yuzuncu Yil University, in 2022. In the experiment, Amaranthus albus L. and four different putrescine doses (0, 50, 100, and 150 mM) were used. The parameters investigated included total antioxidant activity, total phenolic content, total flavonoid content, ascorbic acid, total carotenoid, malondialdehyde (MDA), chlorophyll a, chlorophyll b, and total chlorophyll content. As a result of the study, it was found that the effect of putrescine doses on the investigated parameters, except for chlorophyll a and total chlorophyll content, was statistically significant. The highest antioxidant activity (19.66 µmol TE/ g), total phenolic content (108.0 mg GAE /g), total flavonoid content (130.37 mg QE /100g), ascorbic acid content (22.58 mg LAA /100g), and total carotenoid content (5.35 μg/g) were determined in the 50 mM putrescine treatments. The highest MDA content was found in the control plots, while the highest chlorophyll b content (13.76 μg/g) was observed in the 100 mM putrescine treatments. The lowest values were generally observed in the control plots. Putrescine treatments had a positive and effective impact on the investigated parameters compared to the control. The fact that the MDA content decreased as the dose increased compared to the control indicates that the doses used on the plant did not cause any stress..

Anahtar Kelimeler

Biochemical content, amaranthus albus L., chlorophyll, putrescine, medicinal plant

Kaynakça

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