Onosma heterophylla'nın Antioksidan, Yara İyileştirici ve Sitotoksik Özellikleri

Yıl 2025, Cilt: 15 Sayı: 4, 1219 - 1228

Laçine Aksoy , Ömer Hazman , Ahmet Büyükben , Şevval Candan , Emine Coşkun , Hanife Sena Arslan , Feyza Efiloğlu

https://doi.org/10.21597/jist.1551104

Öz

Çalışmada, Onosma heterophylla’nın toprak üstü kısımları kullanılarak hazırlanan sulu ekstraktların antioksidan, antikarsinojenik ve yara iyileştirici aktivitelerinin belirlenmesi amaçlanmıştır. Sulu ekstraktlar infüzyon ve maserasyon yöntemleri kullanılarak hazırlanmıştır. Ekstraktların antioksidan aktivitesini belirlemek için toplam fenolik madde içeriği, total antioksidan ve oksidan kapasitesi ile oksidatif stres indeks düzeyleri belirlenmiştir. Sitotoksik aktiviteyi belirlemek için L929 hücreleri ve A549 hücreleri kullanılmıştır. L929 hücreleri yara modelinde kullanılmıştır. O. heterophylla'nın aynı familyadaki türlere benzer antioksidan özelliklere sahip olduğu görülmektedir. Bu türle ilişkili en önemli biyolojik aktivite antikarsinojenik ve yara iyileştirici etkidir. Uygulanan hiçbir dozun sağlıklı L929 hücrelerinde hücre canlılığını azaltıcı bir etkisi yoktur. 500-1000 µg/mL konsantrasyonunda sağlıklı hücrelere zarar vermeden kanserli hücrelerin canlılığını azaltırken, 125-250 µg/mL konsantrasyonunda sağlıklı hücrelerin canlılığını artırmış kanserli hücrelerin canlılığını azaltmıştır, bu da çok etkili antikarsinojenik etkiye sahip fitokimyasallar içeren bir bitki olduğunu göstermektedir. O. heterophylla özütünün yara üzerinde %83.72±2.53 oranında kapanma oranına sahip olduğu gözlenmiştir. Gelecekteki çalışmalarda bitkinin biyolojik aktivitelerinden sorumlu bileşen, özellikle antikarsinojenik ve yara iyileştirici etkileri analiz edilebilir.

Anahtar Kelimeler

A549 , Antikanserojen , Hücre çiziği , L929 , Onosma heterophylla

Antioxidant, Wound Healing and Cytotoxic Properties of Onosma heterophylla

Öz

The study was aimed to determine the antioxidant, anticarcinogenic and wound healing activities of aqueous extracts prepared using Onosma heterophylla aerial parts. Aqueous extracts were prepared using infusion and maceration methods. Total phenolic substance content, total antioxidant and oxidant capacity with oxidative stress index levels were determined for to state the antioxidant activity. L929 cells and A549 cells were used to determine cytotoxic activity. L929 were used in the wound model. It is seen that the O. heterophylla has antioxidative properties like the species in the same family. The most important biological activity associated with this species is the anticarcinogenic and wound healing effect. No dose applied has a decreasing effect on cell viability on healthy L929 cells. While it reduces the viability of cancerous cells without harming healthy cells at a concentration of 500-1000 µg/mL, it increases the viability of healthy cells and reduces the viability of cancerous cells at a concentration of 125-250 µg/mL, indicating that it is a plant containing phytochemicals with a very effective anticarcinogenic effect. It was observed that O. heterophylla extract had a closure rate of 83.72±2.53% on the wound. In future studies, the component responsible for the biological activities of the plant, especially its anticarcinogenic and wound healing effects, can be analyzed.

Anahtar Kelimeler

A549 , Anticarcenogenic , Cell scratch , L929 , Onosma heterophylla

Kaynakça

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