Investigation of the Effects of Sinapic Acid Against LeadInduced Spleen Toxicity

Öz

This study aimed to examine the effects of sinapic acid (SA), a natural herbal compound containing phenolic acid, against lead (Pb)-induced spleen toxicity in male rats. Spleen toxicity was assessed following oral treatment of rats with Pb alone or in combination with SA for 7 days. Tissue malondialdehyde (MDA) levels, glutathione (GSH) levels, and the activities of glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) were determined biochemically. While the MDA levels increased in the Pbadministered group in the spleen tissue, GSH levels and the activities of GPx, SOD and CAT decreased. It was observed that co-administration of SA and Pb decreased MDA levels and increased GSH levels as well as GPx, SOD, and CAT activities. It also showed that Pb significantly increased the levels of NF-κB, TNF-α, IL-1β, COX-2, Beclin-1 and caspase3. Compared to the control and Pb groups, SA treatment was determined to significantly reduce the levels of NF-κB, TNF-α, IL-1β, COX-2, Beclin-1 and caspase-3. As a result, in this study, it was determined that SA has protective properties against Pb-induced spleen damage.

Anahtar Kelimeler

• Antioxidant
• Lead
• Oxidative Stress
• Sinapic Acid
• Spleen.

Kurşun Kaynaklı Oluşan Dalak Toksisitesine Karşı Sinapik Asitin Etkilerinin İncelenmesi

Öz

Bu çalışma, fenolik asit içeren doğal bitkisel bileşik olan sinapik asidin (SA), erkek ratlarda kurşun (Pb) kaynaklı dalak toksisitesine karşı etkilerini incelemeyi amaçladı. Dalak toksisitesi, ratların 7 gün boyunca sadece Pb ya da Pb ile kombinasyon halinde SA’nın oral tedavisini takiben değerlendirildi. Doku malondialdehit (MDA) seviyeleri, glutatyon (GSH) seviyeleri, glutatyon peroksidaz (GPx), süperoksit dismutaz (SOD) ve katalaz (KAT) aktiviteleri biyokimyasal olarak belirlendi. Dalak dokusunda Pb uygulanan grupta MDA düzeyi artarken, GSH seviyeleri ve GPx, SOD, KAT aktiviteleri azaldı. Pb ve SA’nın birlikte uygulanmasının MDA düzeyini azalttığı, GSH seviyeleri ve GPx, SOD, KAT aktivitelerini arttırdığı gözlemlendi. Ayrıca Pb'nin NF-κB, TNF-α, IL-1β, COX-2, Beklin-1 ve kaspaz-3 seviyelerini önemli ölçüde artırdığını gösterdi. Kontrol ve Pb grubuyla karşılaştırıldığında ise, SA tedavisinin NF-κB, TNF-α, IL-1β, COX-2, Beklin-1 ve kaspaz-3 seviyelerini önemli ölçüde azalttığı belirlendi. Sonuç olarak bu çalışmada SA'nın Pb kaynaklı dalak hasarına karşı koruyucu özelliğe sahip olduğu belirlendi.

Anahtar Kelimeler

• Antioksidan
• dalak
• kurşun
• oksidatif stres
• sinaptik asit

Kaynakça

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