Ratlarda 3-Asetilpyridin ile Oluşturulan Ataksi Modelinde Aposinin'in İskemi Modifiye Albümin (İMA) Düzeyleri Üzerindeki Etkisi

Öz

Serebellar ataksi koordinasyon bozukluğuna sebep olan nörodejenereatif bir hastalıktır. Bunun gibi nörodejeneratif hastalıklarda apocynin (APO) gibi bileşiklerin tedavi edici rolü bulunmaktadır. Yapılan çalışmanın amacı, 3-asetilpyridin (3-AP) ile oluşturulan serebellar ataksi modelinde APO tedavisinin iskemi modifiye albümin (İMA) seviyesi üzerindeki etkisini incelemektir. Çalışmada, 200–250 gr ağırlığında Sprague-Dawley cinsi, 5-6 haftalık erkek ratlar kullanıldı. Ratlar, Kontrol grubu, 3-AP grubu, 3-AP + APO grubu ve APO grubu olmak üzere dört gruba ayrıldı. 3-AP uygulaması ile ratlarda serebellar ataksi modeli oluşturuldu ve tedavi için APO uygulandı. Deney sonunda anestezi altında dekapitasyon gerçekleştirilerek alınan beyin dokularında İMA seviyeleri ölçüldü. Tüm gruplara göre 3-AP uygulanan ratlarda İMA düzeylerinde istatistiksel olarak anlamlı artış saptanırken, APO uygulanan ratlarda İMA seviyelerinde istatistiksel olarak anlamlı azalış tespit edildi (p<0.001). Son yıllarda biomarker olarak İMA düzeylerinin kullanılabilirliği yönünde birçok çalışma olsada yapılan literatür taramalarında APO’nun 3-AP uygulaması sonucu serebellar ataksi gibi nörodejeneratif hastalıklarda terapötik bir bileşik olarak kullanılabileceğine yönelik beyin İMA düzeylerine bakılan herhangi bir literature rastlanılmadığı için yapılan çalışma literatüre öncü çalışma olarak sunulmuştur. Elde edilen bulgular yapılacak çalışmalar için ışık tutar niteliktedir.

Anahtar Kelimeler

• Antioksidan
• aposinin
• ataksi
• beyin
• İMA

The Effect of Apocynin on Ischemia Modified Albumin (IMA) Levels in the Ataxia Model Induced with 3-Acetylpyridine in Rats

Öz

Cerebellar ataxia is a neurodegenerative disease that causes coordination disorder. In such neurodegenerative diseases, compounds such as apocynin (APO) have a therapeutic role. The aim of this study is to examine the effect of APO treatment on ischemia modified albumin (IMA) level in the cerebellar ataxia model induced by 3-acetylpyridine (3-AP). In the study, 5–6-week-old male Sprague-Dawley rats weighing 200-250 grams were used. Rats were divided into four groups: Control group (K), 3- 3-AP group, 3-AP + APO, and APO group. A cerebellar ataxia model was induced in rats using 3-AP administration, and APO was administered as a treatment. At the end of the experiment, decapitation was performed under anesthesia, and IMA levels were measured in the brain tissues obtained. In rats administered with 3-AP, there was a statistically significant increase in IMA levels compared to all groups, whereas rats treated with APO showed a statistically significant decrease in IMA levels (p<0.001). Although many studies in recent years have investigated the usability of IMA levels as biomarkers, literature searches did not reveal any studies examining the therapeutic potential of APO in neurodegenerative diseases like cerebellar ataxia induced by 3-AP, in terms of brain IMA levels. Therefore, this study is presented as a pioneering contribution to the literature. The findings obtained shed light on future research.

Anahtar Kelimeler

• Antioxidant
• apocynin
• ataxia
• brain
• IMA

Kaynakça

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