Sıçanlarda Diklofenak ile Oluşturulan Böbrek Toksisitesinde Rutin’in Etkilerinin Histopatolojik ve Biyokimyasal Olarak Araştırılması

Öz

Antiinflamatuar, antipiretik ve analjezik özellikleriyle bilinen nonsteroid antiinflamatuar ilaç (NSAID) kategorisinde yer alan diklofenak (DCL), uzun süreli kullanımı dokularda oksidatif stres ve inflamasyon artışına sebep olarak toksik etki oluşturur. Rutin (RUT), birçok bitkide doğal olarak bulunan anti-oksidan, anti-inflamatuar ve anti-apoptotik etkilere sahip bir flavanoid glikozidtir. Bu çalışmada, doğal bir antioksidan olan RUT’nin DCL kaynaklı böbrek doku hasarı üzerine etkilerinin araştırılması amaçlanmıştır. 28 adet wistar albino cinsi sıçan kontrol, DCL, RUT, DCL50 + RUT100 grupları olmak üzere 4 gruba ayrıldı. 4 gün boyunca 100 mg/kg RUT uygulaması oral yolla verilerek bununla birlikte 3. ve 4. günlerde 50 mg/kg dozda DCL uygulaması intraperitoneal yolla yapıldı. 5. günde böbrek dokuları alındı ve PCR (Polimeraz Zincir Reaksiyonu) yöntemi ile oksidatif stres, inflamasyon ve apoptotik belirteçlerin analizi ve dokuların histopatolojik analizi yapıldı. Böbrek dokularında DCL kaynaklı oksidatif stres, inflamasyon ve apoptoz parametrelerin düzeyleri kontrol grubuna göre artmıştır. RUT uygulamasıyla birlikte DCL bağlı tüm bu artış düzeylerinde azalmalar meydana gelmiştir. Böbrek dokularında DCL maruziyetinin sebep olduğu toksisiteye karşı RUT’nin potansiyel koruyucu etkiye sahip olduğu sonucuna varıldı.

Anahtar Kelimeler

• Diklofenak
• Nefrotoksisite
• Rutin
• Sıçan

Histopathological and Biochemical Investigation of the Effects of Rutin on Diclofenac-Induced Renal Toxicity in Rats

Öz

Diclofenac (DCL), known for its anti-inflammatory, antipyretic, and analgesic properties, belongs to the category of nonsteroidal anti-inflammatory drugs (NSAIDs) and can cause toxic effects due to increased oxidative stress and inflammation in tissues with long-term use. Rutin (RUT) is a flavonoid glycoside that naturally occurs in many plants and possesses antioxidant, anti-inflammatory, and anti-apoptotic effects. This study aimed to investigate the effects of RUT, a natural antioxidant, on DCL-induced kidney tissue damage. A total of 28 Wistar albino rats were divided into 4 groups: control, DCL, RUT, and DCL50 + RUT100. For 4 days, 100 mg/kg of RUT was administered orally, and on the 3rd and 4th days, 50 mg/kg of DCL was administered intraperitoneally. On the 5th day, kidney tissues were collected, and the analysis of oxidative stress, inflammation, and apoptotic markers was performed using the PCR (Polymerase Chain Reaction) method, along with histopathological analysis of the tissues. The levels of oxidative stress, inflammation, and apoptosis parameters in kidney tissues were found to be increased in the DCL group compared to the control group. The administration of RUT resulted in reductions in all these elevated levels associated with DCL. It was concluded that RUT has a potential protective effect against the toxicity caused by DCL exposure in kidney tissues.

Anahtar Kelimeler

• Diclofenac
• Nephrotoxicity
• Rutin
• Rat.

Kaynakça

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