Ratlarda Etilen Glikol Deneysel Nefrolitiazis Modelinde Amonyum Kloridin Etkileri ve Nefrolitiaziste S100A4 Ekspresyonu

Yıl 2026, Cilt: 6 Sayı: 1, 47 - 52, 24.03.2026

Hasan Erdoğan , Tuncer Kutlu , Ufuk Kaya

https://doi.org/10.62425/jlasp.1832766

https://izlik.org/JA95RB45FF

Öz

Böbrek taşlarının oluşum mekanizmasını in vivo olarak incelemek için fareler, tavşanlar, ratlar ve domuzlar dahil olmak üzere çeşitli hayvan modelleri geliştirilmiştir. Bu çalışmada farklı sürelerde (7 ve 14 gün) etilen glikol (EG) (%1) ve amonyum klorid’in (AK) (% 0,5, %1 ve %2) birlikte nefrolitiazis oluşturulması modelinde AK’nın etkisinin saptanması hedeflendi. Çalışmada her grupta 7 adet erkek Wistar Albino rat olacak şekilde 9 grup oluşturuldu. Histopatolojik inceleme için Hematoksilen Eozin ve Pizzalato boyaması yapıldı. S100A4 ekpresyonunun immunohistokimyasal incelemesinde, Avidin-Biotin Complex Peroksidase yöntemi uygulandı. Yaptığımız çalışmada içme suyuna %1 EG ile birlikte %2 AK ilavesi yapılan grupta ratların çoğunun öldüğü ve %2 AK ilavesinin bu tip çalışmalara uygun olmadığı belirlendi. Bu çalışmada %1 EG tek başına 7 ya da 14 gün verildiğinde ya da buna AK %0,5- %1 ve %2 dozlarında 7 gün verildiğindekristal birikimi ve histopatolojik değişiklikler açısından kontrol grubuna göre istatistiki olarak anlamlı değişklikler oluşmadığı saptandı. Çalışmada kristal birikimi, yangısal hücre infiltrasyonu ve tubul epitellerinde hidropik dejenerasyon gibi önemli değişiklikler ancak içme suyuna %1 EG ile birlikte %0,5 veya %1 oranında AK 14 gün verildiğinde tespit edildi. Bu gruplarda ayrıca yangısal hücrelerde ve intersitisyel fibroblastlarda S100A4 ekpresyonunda anlamlı artış olduğu belirlendi.

Anahtar Kelimeler

Amonyum klorid , Etilen glikol , Nefrolitiazis , Rat

Proje Numarası

24.YL.008

Effects of Ammonium Chloride in the Ethylene Glycol Experimental Nephrolithiasis Model in Rats

https://izlik.org/JA95RB45FF

Öz

Various animal models, including mice, rabbits, rats and pigs, have been developed to study the formation mechanism of kidney stones in vivo. This study aimed to assess the effect of ammonium chloride in a nephrolithiasis model induced by ethylene glycol (EG) (1%) combined with ammonium chloride (AC) at different doses (0.5%, 1%, and 2%) over various durations (7 and 14 days). The study consisted of nine groups, each consisting of seven male Wistar Albino rats. Histopathological analysis was performed using Hematoxylin-Eosin and Pizzolato’s special staining. It was observed that most rats in the group receiving 2% AC in drinking water alongside 1% EG died, indicating that 2% AC is unsuitable for such studies. The results showed that administering 1% EG alone for 7 or 14 days, or giving AC at doses of 0.5%, 1%, and 2% for 7 days, did not produce statistically significant differences compared to the control group regarding crystal deposition and histopathological changes (p < .002). Significant changes, such as crystal accumulation, inflammatory cell infiltration, and hydropic degeneration of tubular epithelium, were observed only when 0.5% or 1% AC was added to drinking water with 1% EG for 14 days (p < .002). 

Anahtar Kelimeler

Ammonium chloride , Ethylene glycol , Nephrolithiasis , Rat

Etik Beyan

This study was done with Hatay Mustafa Kemal University Animal Experiments Local Ethics Committee permission (Approval No: 2024/01-04, Date: 26.01.2024)

Destekleyen Kurum

This study was financially supported by the Scientific Research Projects Fund of Hatay Mustafa Kemal University (Project number: 24.YL.008).

Proje Numarası

24.YL.008

Kaynakça

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