Sıçanlarda Testis Torsiyon/Detorsiyon Hasarına Karşı Umbelliferon ve Naringinin İnflamatuar ve Steroidogenik Belirteçler Üzerindeki Etkilerinin Değerlendirilmesi

Öz

Bu çalışma, bir sıçan modelinde torsiyon/detorsiyon (T/D) kaynaklı testis hasarına karşı, her biri iki farklı doz seviyesinde (50 ve 100 mg/kg) uygulanan umbelliferon ve naringinin koruyucu etkilerini değerlendirmiş ve karşılaştırmıştır. Kırk iki yetişkin erkek Sprague-Dawley sıçanı rastgele yedi gruba (her biri n=6) ayrılmıştır: kontrol, Sham, T/D ve torsiyon sonrası yedi gün boyunca umbelliferon (50 ve 100 mg/kg) veya naringin (50 ve 100 mg/kg) ile tedavi edilen T/D grupları. Sol testis torsiyonu oluşturulmuş (720°, 2 saat iskemi) ve bunu takiben 7 günlük reperfüzyon süreci uygulanmıştır. Testis histolojisi (H&E), NF-κB immünohistokimyası ve TNF-α, IL-1β, IL-8, StAR, 3β-HSD ve 17β-HSD'nin serum ELISA düzeyleri değerlendirilmiştir. T/D; kontrollere kıyasla belirgin seminifer tübül düzensizliğine, dejenere spermatidlere ve artmış NF-κB immünopozitifliğine yol açmıştır. Ayrıca T/D, kontrole kıyasla TNF-α, IL-1β ve IL-8 düzeylerini anlamlı derecede yükseltmiş; StAR, 3β-HSD ve 17β-HSD düzeylerini ise baskılamıştır. Hem umbelliferon hem de naringin, her iki dozda da T/D grubuna kıyasla seminifer tübül yapısını korumuş, NF-κB immünoreaktivitesini azaltmış ve her üç pro-inflamatuar sitokinin düzeyini de anlamlı ölçüde düşürmüştür. 17β-HSD düzeyleri her iki bileşiğin her iki dozuyla da anlamlı ölçüde iyileştirilmiş; 3β-HSD ve StAR için de istatistiksel olarak anlamlı olmasa da iyileşme yönünde benzer bir eğilim gözlenmiştir. Umbelliferon ve naringin; NF-κB aktivasyonunu, pro-inflamatuar sitokin salınımını ve steroidojenik enzim baskılanmasını hafifleterek T/D kaynaklı testis hasarına karşı kıyaslanabilir ve anlamlı bir koruma sağlamakta; böylece testis iskemi-reperfüzyon hasarına karşı koruyucu ajanlar olma potansiyellerini desteklemektedir.

Anahtar Kelimeler

• Umbelliferon
• Testis Torsiyonu
• Steroidogenez
• Naringin
• NF-kappa B

Evaluation of the Effects of Umbelliferone and Naringin on Inflammatory and Steroidogenic Markers Against Testicular Torsion/Detorsion Injury in Rats

Öz

This study evaluated and compared the protective effects of umbelliferone and naringin, each administered at two dose levels (50 and 100 mg/kg), against torsion/detorsion (T/D)-induced testicular injury in a rat model. Forty-two adult male Sprague-Dawley rats were randomly allocated into seven groups (n=6 each): control, Sham, T/D, and T/D treated with umbelliferone (50 and 100 mg/kg) or naringin (50 and 100 mg/kg) for seven consecutive days after torsion. Left testicular torsion was induced (720°, 2 h ischemia) followed by a 7-day reperfusion. Testicular histology (H&E), NF-κB immunohistochemistry, and serum ELISA levels of TNF-α, IL-1β, IL-8, StAR, 3β-HSD, and 17β-HSD were assessed. T/D produced marked seminiferous tubule disorganization, degenerated spermatids, and increased NF-κB immunopositivity compared with controls. T/D also significantly elevated TNF-α, IL-1β, and IL-8 and suppressed StAR, 3β-HSD, and 17β-HSD levels relative to control. Both umbelliferone and naringin, at either dose, preserved seminiferous tubule structure, reduced NF-κB immunoreactivity, and significantly lowered all three pro-inflammatory cytokines compared with the T/D group. 17β-HSD levels were significantly restored by both compounds at both doses, with a parallel, though not statistically significant, trend toward restoration observed for 3β-HSD and StAR. Umbelliferone and naringin confer comparable, meaningful protection against T/D-induced testicular injury by attenuating NF-κB activation, pro-inflammatory cytokine release, and steroidogenic enzyme suppression, supporting their potential as protective agents against testicular ischemia-reperfusion injury.

Anahtar Kelimeler

• Naringin
• NF-kappa B
• Steroidogenesis
• Testicular torsion
• Umbelliferone

Kaynakça

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