Oksaliplatin Kaynaklı Testis Hasarı Üzerine Likopenin Etkilerinin Araştırılması

Yıl 2022, Cilt: 2 Sayı: 1, 49 - 55, 28.03.2022

Sefa Küçükler Fatih Mehmet Kandemir

Öz

Oksaliplatin (Olp) ilerlemiş kolorektalkanserlerin tedavisinde yaygın olarak kullanılan bir ilaçtır. Diğer platin türevlerine göre oksaliplatin, sadece hafif bir hematolojik ve gastrointestinal toksisiteye neden olmaktadır. Domateslerde ve ürünlerindebulunan asiklik bir hidrokarbon karotenoidolan likopen (Lcp), güçlü bir antioksidandır ve hayvan modellerinde antikanser özellikleri gösterilmiştir. Bu çalışma sıçanlarda Lcp’nin Olp ile indüklenen testis toksisitesine karşı koruyucu etkilerini değerlendirmeyi amaçlamaktadır. Araştırmada erkek Sprague Dawley sıçanları kullanıldı ve 5 deney grubu oluşturuldu: 1-kontrol grubu, 2-Likopen (Lcp) uygulanan grup, 3-Oksaliplatin (Olp) uygulanan grup, 4-Oksaliplatin + likopen 2 mg/kg (Olp+Lcp2) uygulanan grup ve 5-oksaliplatin + likopen 4 mg/kg (Olp+Lcp4) uygulanan grup. 1 ve 2. günler ile 5 ve 6. günler likopen uygulamasından 30 dakika sonra oksaliplatin 4 mg/kg dozunda %5 dektrozda çözülerek i.p. olarak uygulandı. Doku malondialdehit (MDA), glutatyon (GSH) düzeyleri, glutatyon peroksidaz (GPx), süperoksit dismutaz (SOD) ve katalaz (KAT) aktiviteleri biyokimyasal olarak belirlendi. Testis dokusunda oksaliplatin grubunda MDA düzeyleri yükselirken, GSH, GPx, SOD ve Kat değerleri azaldı. Likopenin oksaliplatin ile uygulanan farklı dozları ise MDA düzeyini azaltırken, GSH, GPx, SOD ve KAT aktivitelerini artırdı. Bu çalışma ile oksaliplatin ile oluşturulan testis hasarına karşı likopenin koruyucu özelliğinin olduğu tespit edilmiştir.

Anahtar Kelimeler

Likopen, oksaliplatin, oksidatif Stres, rat

Investigation Of The Effects Of Lycopene On Oxaliplatin-Induced Testicular Damage

Öz

Oxaliplatin (Olp) is a drug widely used in the treatment of advanced colorectal cancers. Compared to other platinum derivatives, oxaliplatin causes only mild haematological and gastrointestinal toxicity. Lycopene (Lcp), an acyclic hydrocarbon carotenoid found in tomatoes and their products, is a potent antioxidant and has been shown to have anticancer properties in animal models. This study aims to evaluate the protective effects of Lcp against Olp-induced testicular toxicity in rats. Male Sprague Dawley rats were used in the study and 5 experimental groups were formed: 1-control group, 2-Lycopene (Lcp) administered group, 3-Oxaliplatin (Olp) administered group, 4-Oxaliplatin + lycopene 2 mg/kg (Olp+Lcp2) administered group. group and 5-oxaliplatin + lycopene 4 mg/kg (Olp+Lcp4) administered group. On days 1 and 2, and days 5 and 6, 30 minutes after lycopene administration, oxaliplatin is dissolved in 5% dextrose ata dose of 4 mg/kg and administered i.p. was applied as Tissue malondialdehyde (MDA), glutathione (GSH) levels, glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (Cat) activities were determined biochemically. While MDA levels increasedin testicular tissue in the oxaliplatin group, GSH, GPx, SOD and CAT values decreased. Different doses of lycopene administered with oxaliplatin decreased MDA levels and increased GSH, GPx, SOD and CAT activities. In this study, it was determined that lycopene has a protective feature against testicular damage induced by oxaliplatin.

Anahtar Kelimeler

Lycopene, oxaliplatin, oxidative Stress, rat

Kaynakça

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