Sıçanlarda Çekal Ligasyon ve Delme Kaynaklı Sepsis Modeli

Yıl 2022, Cilt: 2 Sayı: 2, 81 - 89, 28.09.2022

Mustafa Can Güler Ayhan Tanyeli Ersen Eraslan Selim Çomaklı Yasin Bayır

Öz

Sepsis, yüksek ölüm oranına sahip dünya çapında bir sağlık sorunudur. Travma, cerrahi ve enfeksiyon gibi çeşitli durumlardan kaynaklanabilir. Böbrek ve akciğerler sepsise bağlı hasarlanan organlar arasında yer alır. Çekal ligasyon ve delme (CLP) klinik sepsise benzerdir. Klinik divertikülit veya apandisit perforasyonlarını taklit etmek için tercih edilir. Bu çalışmayı, ileride gerçekleştirmeyi planladığımız CLP ile indüklenen sepsise karşı koruyucu ajan çalışmaları için bir ön çalışma olarak tasarladık. Literatürde bulunan deneysel CLP modelleriyle uyumlu biyokimyasal ve histolojik sonuçları bulmayı hedefledik. Çalışmada 20 adet dişi Wistar Albino sıçan kullandık. Rastgele iki grup oluşturduk; sham grubu ve CLP grubu (n=10). İnterlökin 1-beta (IL-1β), IL-6 ve IL-10 sitokin seviyelerini ölçtük. Böbrek ve akciğer dokularında miyeloperoksidaz (MPO), süperoksit dismutaz (SOD), malondialdehit (MDA) ve glutatyon (GSH) düzeylerini belirledik. Ayrıca doku örneklerini histopatolojik ve immünohistokimyasal değerlendirme için inceledik. CLP grubunda sepsise bağlı güçlü bir immün yanıtın göstergesi olarak sitokin seviyeleri ve oksidan parametreleri artarken antioksidan parametreler azaldı. Ayrıca, histopatolojik ve immünohistokimyasal bulgular biyokimyasal bulguları sepsis lehine destekledi. Elde ettiğimiz sonuçlar ileride CLP ile indüklenen sepsis modeli olarak kullanabileceğimiz başarılı bir CLP modelini gösteren anlamlı bir sepsis tablosu oluşturdu.

Anahtar Kelimeler

Çekal ligasyon ve delme, sepsis, sıçan

Destekleyen Kurum

Atatürk Üniversitesi

Proje Numarası

8791

Cecal Ligation and Puncture-Induced Sepsis Model in Rats

Öz

Sepsis is a worldwide health problem with a high mortality rate. It may result from various conditions, including trauma, surgery, and infection. Kidneys and lungs rank among the sepsis-induced injured organs. Cecal ligation and puncture (CLP) is similar to clinical sepsis and is preferred to imitate clinical diverticulitis or appendicitis perforations. Here, we designed a CLP model as a prestudy for further protective agent studies against CLP-induced sepsis. We aimed to find biochemical and histological results compatible with previous experimental CLP models in the literature. We used 20 female Wistar Albino rats. We created two randomized groups, a sham group, and a CLP group (n=10). We measured interleukin 1-beta (IL-1β), IL-6, and IL-10 cytokine levels. We assessed myeloperoxidase (MPO), superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione (GSH) levels in renal and lung tissues. We also examined the tissue samples for histopathological and immunohistochemical evaluation. Cytokine levels and oxidant parameters were increased, and antioxidant parameters decreased in the CLP group as an indicator of a strong immune response due to sepsis. Histopathological and immunohistochemical findings supported the biochemical findings on behalf of sepsis. The results demonstrated a meaningful sepsis picture indicating a successful CLP model, which we may prefer to use in further CLP-induced sepsis models.

Anahtar Kelimeler

Cecal ligation and puncture, rat, sepsis

Proje Numarası

8791

Kaynakça

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