Laboratuvar Hayvanlarında Tarama Programlarının Önemi ve Bazı Önemli Viral Ajanların Araştırılması

Yıl 2024, Cilt: 4 Sayı: 2, 91 - 100, 19.09.2024

Erdal Polat , Hayrunnisa Bostan Yörü , Yasin Kalay , Sebahattin Akça

https://doi.org/10.62425/jlasp.1484805

Öz

Laboratuvar hayvanlarının sağlık tarama programları, biyomedikal araştırmalarda standardizasyonun sağlanması ve hem hayvanların hem de araştırma personelinin sağlığının korunması açısından büyük önem taşımaktadır. Bu bağlamda, Hepatit E virusu (HEV), Murine hepatit virusu (MHV) ve Murine norovirus (MNV) gibi patojenlerin düzenli olarak izlenmesi, laboratuvar ortamında hijyen ve sanitasyon standartlarının geliştirilmesine katkıda bulunabilir. Araştırmada, BALB/c mice ve Sprague Dawley, Wistar albino sıçanlarından oluşan rastgele seçilmiş örneklem grupları üzerinde çalışılmıştır. Deney hayvanlarından etik ilkeler çerçevesinde kan ve doku örnekleri toplanmış, laboratuvar ortamındaki ses, ışık, kafes yoğunluğu, yem ve suya erişim gibi faktörler gözlemlenerek kaydedilmiştir. Bu gözlemler, araştırmanın güvenilirliğini ve tekrarlanabilirliğini artırmak için elzemdir. RT-PCR testleri kullanılarak HEV, MHV ve MNV için tarama yapılmış ve test edilen tüm örnekler bu patojenler yönünden negatif bulunmuştur. Bu sonuçlar, laboratuvar ortamında uygulanan hijyen ve sanitasyon protokollerinin etkinliğini göstermektedir. Ayrıca, laboratuvar araştırma merkezlerinde eğitimli personel ve kemirgen kontrol programlarının önemi vurgulanmıştır. Sonuç olarak, bu çalışma, laboratuvar hayvanlarında sağlık tarama programlarının, araştırılan patojenler ve uygulanan koruma-kontrol tedbirleri ile ilişkili olarak, bilimsel araştırmaların standardını yükseltmede kritik bir rol oynadığını ortaya koymaktadır. Bu tür programlar, laboratuvar hayvanlarının sağlığını korumak ve araştırma sonuçlarının güvenilirliğini artırmak için hayati öneme sahiptir.

Anahtar Kelimeler

HEV, MNV, MHV, sağlık tarama programları, deney hayvanları

The Importance of Screening Programs in Laboratory Animals and Investigation of Several Important Viral Agents

Öz

Laboratory animal health screening programs are of great importance to ensure standardization in biomedical research and to protect the health of both animals and research personnel. In this
context, regular monitoring of pathogens such as Hepatitis E virus (HEV), Murine hepatitis virus (MHV) and Murine norovirus (MNV) can contribute to improving hygiene and sanitation standards
in the laboratory environment. In this study, randomly selected sample groups consisting of BALB/c mice and Sprague Dawley, Wistar albino rats were studied. Blood and tissue samples were collected from the laboratuary animals within the framework of ethical principles, and factors such as noise, light, cage density, access to feed and water in the laboratory environment were
observed and recorded. These observations are essential to increase the reliability and reproducibility of the study. RT-PCR tests were used to screen for HEV, MHV and MNV and all samples tested were negative for these pathogens. These results demonstrate the effectiveness of hygiene and sanitation protocols applied in the laboratory environment. Furthermore, the importance of trained personnel and rodent control programs in laboratory research centers was emphasized. In conclusion, this study reveals that health screening programs in laboratory animals play a critical role in raising the standard of scientific research in relation to the pathogens investigated and the protection-control measures implemented. Such programs are vital to protect the health of laboratory animals and improve the reliability of research results.

Anahtar Kelimeler

HEV, MNV, MHV, health screening programs, experimental animals.

Kaynakça

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