Karaman İlinde Tüketilen Balıklarda Ağır Metal Birikimi ve İnsan Sağlığı Üzerindeki Etkileri

Öz

Ağır metaller, çevrede birikebilme yetenekleri ile su ekosistemine ve balık tüketimi yoluyla da insanlara taşınabilirler. Biyolojik olarak parçalanamadıkları için ağır metallerin insan sağlığına ciddi zarar verme potansiyeli bulunmaktadır. Karaman ilinin su ürünleri üretimi potansiyelinin yetersiz olması su ürünlerinin farklı bölgelerden gelmesine yol açmıştır. Farklı ekolojik bölgelerden gelen balık türlerinin metal seviyeleri ile ilgili Karaman ilinde daha önce bir çalışma yapılmamıştır. Karaman ilinde 2023 yılında yerel pazardan alınan halkın çok tükettiği sekiz balık türünün (gökkuşağı alabalığı (Oncorhynchus mykiss), barbun (Mullus barbatus), çipura (Sparus aurata), hamsi (Engraulis encrasicolus), istavrit (Trachurus mediterraneus), kupes (Boops boops), levrek (Dicentrarchus labrax) ve mercan (Pagrus pagrus)) kas metal biyobirikimleri ve tüketiminin insan sağlığı üzerindeki olası etkileri araştırılmıştır. Metal konsantrasyonları (As, Cd, Cr, Cu, Fe, Mn, Ni ve Zn) İndüktif Eşleşmiş Plazma - Optik Emisyon Spektrometresi (ICP-OES) cihazıyla belirlenmiştir. Tespit edilen metal konsantrasyonları ulusal ve uluslararası yönetmeliklerin maksimum izin verilebilir limitleri ile kıyaslanmıştır. İnorganik arsenik (iAs) ortalama konsantrasyonun mercan (0,233 mg/kg) balığında, kadmiyum (Cd) ortalama konsantrasyonlarının ise istavrit (0,060 mg/kg), kupes (0,063 mg/kg) ve mercan (0,171 mg/kg) balıklarında yönetmeliklerin maksimum izin verilebilir limitlerini aştığı bulunmuştur. Sağlık riski değerlendirme yöntemleri (Tahmini Günlük Alım, Hedef Tehlike Oranı, Tehlike İndeksi), incelenen balık türlerinin tüketiminin güvenli olduğunu göstermiştir.

Anahtar Kelimeler

• Karaman
• ağır metal
• balık tüketim
• sağlık risk değerlendirme
• ICP-OES.

Determination of Heavy Metal Concentrations in Some Fish Species Consumed in Karaman Province, Türkiye

Öz

Heavy metals, with their ability to accumulate in the environment, can be transported to the aquatic ecosystem and to humans through fish consumption. Since they are not biodegradable, heavy metals have the potential to cause serious harm to human health. The insufficient aquaculture production of Karaman province has led to aquaculture products coming from different regions. No previous study has been conducted on the metal levels of fish species from different ecological regions in Karaman province. In 2023, the muscle metal bioaccumulations and the possible health effects of consumption of eight fish species (bogue (Boops boops), European seabass (Dicentrarchus labrax), European anchovy (Engraulis encrasicolus), red mullet (Mullus barbatus), rainbow trout (Oncorhynchus mykiss), red porgy (Pagrus pagrus), gilt-head seabream (Sparus aurata) and Mediterranean horse mackerel (Trachurus mediterraneus)) purchased from the local market were investigated in Karaman province. Metal concentrations (As, Cd, Cr, Cu, Fe, Mn, Ni and Zn) were determined by Inductively Coupled Plasma - Optical Emission spectroscopy (ICP-OES) and their levels were compared with the maximum permissible limits of national and international regulations. It was found that the mean concentrations of cadmium (Cd) in the Mediterranean horse mackerel (0.060 mg/kg), bogue (0.063 mg/kg) and red porgy (0.171 mg/kg), and the mean concentration of inorganic arsenic (iAs) in red porgy (0.233 mg/kg) exceeded the maximum permissible limits of the regulations. Health risk assessment methods (Estimated Daily Intake, Target Hazard Ratio, Hazard Index) have shown that the fish species examined are safe to consume.

Anahtar Kelimeler

• Karaman
• heavy metal
• fish consumption
• health risk assessment
• ICP-OES

Kaynakça

1. Aktop, Y., & Çağatay, İ. T. (2020). Ağır metallerin balıklarda birikimi ve etkileri. Menba Kastamonu Üniversitesi Su Ürünleri Fakültesi Dergisi, 6(1), 37-44.
2. Almafrachi, H. A. A., Gümüş, N. E., & Çorak Öcal, İ. (2024). Heavy metal bioaccumulation in fish: ımplications for human health risk assessment in ten commercial fish species from Konya, Türkiye. International Journal of Environmental Science and Technology. doi: 10.1007/s13762-024-05875-3
3. AOAC. (2016). Appendix F: Guidelines for standard method performance requirements. Erişim tarihi: 13 Mart 2024, https://www.aoac.org/wp-content/uploads/2019/08/app_f.pdf
4. Akturfan, M., & Dağcı, T. (2024). Ramazan Yemeklerinde Sosyo-Kültürel Değişim: Karaman İli Örneği. Anemon Muş Alparslan Üniversitesi Sosyal Bilimler Dergisi, 12(1), 13-31. doi: 10.18506/anemon. 1239749
5. Aşıkkutlu, B., Gümüş, N. E., & Akköz, C. (2021). Water quality properties of.org Acı Lake and Meke Lake (Konya, Turkey). LimnoFish-Journal of Limnology and Freshwater Fisheries Research, 7(3), 260-270. doi: 10.17216/limnofish.799091
6. Bat, L., Şahin, F., Öztekin, A., Arici, E., & Yardim Ö. (2019). Assessment of Cd, Hg, Pb, Cu and Zn amounts in muscles of Cyprinus carpio from Karasu Stream, Sinop. Current Agriculture Research Journal, 7(2), 171–180. doi: 10.12944/carj.7.2.05
7. Brix, K. V., Schlekat, C. E., & Garman, E. R. (2017). The mechanisms of nickel toxicity in aquatic environments: An adverse outcome pathway analysis. Environmental Toxicology and Chemistry, 36, 1128-1137. doi: 10.1002/etc.3706
8. Bosch, A. C., O'Neill, B., Sigge, G. O., Kerwath, S. E., & Hoffman, L.C. (2016) Heavy metals in marine fish meat and consumer health: a review. Agricultural and Food Sciences, Environmental Science, 96(1), 32–48. doi: 10.1002/jsfa.7360

9. Çiftçi, H., Er Çalışkan, Ç., & Öztürk, K. (2021). Bazı balık türlerinde iz ve toksik metal düzeylerinin belirlenmesi ve insan sağlığı riskinin değerlendirilmesi. Journal of Advanced Research in Natural and Applied Sciences, 7(2), 219-233. doi: 10.28979/jarnas.883611
10. Copat, C., Arena, G., Fiore, M., Ledda, C., Fallico, R., Sciacca, S., & Ferrante, M. (2013). Heavy metals concentrations in fish and shellfish from eastern Mediterranean Sea: consumption advisories. Food and Chemical Toxicology, 53, 33-37. doi: 10.1016/j.fct.2012.11.038
11. Cui, B. S., Zhang, Q. J., Zhang, K. J., Liu, X. H., & Zhang, H. G. (2011). Analyzing trophic transfer of heavy metals for food webs in the newly-formed wetlands of the Yellow River Delta, China. Environmental Pollution, 159(5), 1297-1306. doi: 10.1016/j.envpol.2011.01.024
12. Dadar, M., Adel, M., Ferrante, M., Saravi, H. N., Copat, C., & Conti, G. O. (2016). Potential risk assessment of tracemetals accumulation in food, water and edible tissue of rainbow trout (Oncorhynchus mykiss) farmed in Haraz River, Northern Iran. Toxin Reviews, 35, 141–146. doi: 10.1080/15569543.2016.1217023
13. DIN EN ISO 11885. (2009). Water quality Determination of selected elements by inductively coupled plasma optical emission spectrometry (ICP-OES) (ISO 11885: 2007); German version EN ISO, 2009, 11885.
14. Durmaz, E., Kocagöz, R., Bilacan, E., & Orhan, H. (2017). Metal pollution in biotic and abiotic samples of the Büyük Menderes River, Turkey. Environmental Science and Pollution Research, 24, 4274-4283. doi: 10.1007/s11356-016-6417-7
15. EC. (2023). Commission Regulation (EC) No 2023/915 of 25 April 2023: on maximum levels for certain contaminants in food and repealing Regulation (EC) No 1881/2006. Official Journal of the European Union, L 119/103. Erişim tarihi: 01 Haziran 2024, https://eur-lex.europa.eu/eli/reg/2023/915/oj
16. Fallah, A. A., Saei-Dehkordi, S. S., Nematollahi, A., & Jafari, T. (2011). Comparative study of heavy metal and trace element accumulation in edible tissues of farmed and wild rainbow trout (Oncorhynchus mykiss) using ICP-OES technique. Microchemical Journal, 98, 275–279. doi: 10.1016/j.microc.2011.02.007
17. FSANZ. (2024). Australia New Zealand Food Standards Code, Schedule 19 – Maximum levels of contaminants and natural toxicants. Erişim tarihi: 01 Haziran 2024, https://www.legislation.gov.au/F2015L00454/latest/text
18. Guérin, T., Chekri, R., Vastel, C., Sirot, V., Volatier, J. L., Leblanc, J. C., & Noël, L. (2011). Determination of 20 trace elements in fish and other seafood from the French market. Food Chemistry, 127(3), 934-942. doi: 10.1016/j.foodchem.2011.01.061
19. Güler, Ç., & Çobanoğlu, Z. (1997). Su kalitesi (1. Baskı). Ankara: T.C. Sağlık Bakanlığı.
20. Gültürk, M. (2021). Bingöl’de tüketilen bazı balıklarda ağır metal düzeylerinin araştırılması. (Yüksek Lisans Tezi), Munzur Üniversitesi, Tunceli.
21. Gümüş, N. E., & Akköz, C. (2021). Bioaccumulation of heavy metals in the water, sediment and the tissues of Carassius gibelio (Bloch, 1782) from EberLake. Ege Journal of Fisheries and Aquatic Sciences, 38(4), 507-514. doi: 10.12714/egejfas.38.4.12
22. Gümüş, B., & Buluş, A. (2020). Uluslararası çevre sorunları ve William Nordhaus’un çevre ekonomisine katkıları. Alanya Akademik Bakış Dergisi, 4(3), 1015-1031. doi: 10.29023/alanyaakademik.686110
23. Gümüş, N.E., Keskinkaya, H.B., Okudan, E.Ş. & Akköz, C. (2024). Akdeniz Bölgesi (Antalya/ Türkiye) makroalg türlerinin makro ve iz element seviyelerinin belirlenmesi, Necmettin Erbakan Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 6(1), 1-9. doi: 10.47112/neufmbd.2024.28
24. Hongxing, Z., & Yu-Kui, R. (2011). Determination of trace elements, heavy metals and rare earth elements in corn seeds from Beijing by ICP-MS simultaneously. E-Journal of Chemistry, 8(2), 782-786.
25. Kalantzi, I., Mylona, K., Pergantis, S. A., Coli, A., Panopoulos, S., & Tsapakis, M. (2019). Elemental distribution in the different issues of brood stock from Greek hatcheries. Aquaculture, 503, 175–185. doi: 10.1016/j.aquaculture.2019.01.004
26. Kalyoncu, L., Kalyoncu, H., & Arslan, G. (2012). Determination of heavy metals and metals levels in five fish species from Işıklı Dam Lake and Karacaören Dam Lake (Turkey). Environmental Monitoring and Assessment, 184, 2231–2235. doi: 10.1007/s10661-011-2112-9
27. Kavak, S., & Pekerşen, Y. (2022). Yöresel mutfak kültürünün turizme kazandırılmasında yerel halkın tutumu: Karaman İlinde bir araştırma. Seyahat ve Otel İşletmeciliği Dergisi, 19(1), 59-80. doi: 10.24010/soid.1019182
28. Korkmaz, C., Ay, Ö., Ersoysal, Y., Köroğlu, M. A., & Erdem, C. (2019). Heavy metal levels in muscle tissues of some fish species caught from north-east Mediterranean: evaluation of their effects on human health. Journal of Food Composition and Analysis, 81, 1-9. doi: 10.1007/s11270-017-3503-5
29. Li, Y., Wang, X., Xu, H., Xia, P., Wang, H., Jing, H., Li, J., & Zhao, J. (2018). High zinc removal from water and soil using struvite-supported diatomite obtained by nitrogen and phosphate recovery from wastewater. Environmental Chemistry Letters, 16(2), 569-573. doi: 10.1007/s10311-017-0694-3
30. Loghmani, M., Tootooni, M. M., & Sharifian, S. (2022). Risk assessment of trace element accumulation in two species of edible commercial fish Scomberoides commersonnianus and Cynoglossus arel from the northern waters of the Oman Sea. Marine Pollution Bulletin, 174, 113201. doi: 10.1016/j.marpolbul.2021.113201
31. Martins, A. C., Morcillo, P., Ijomone, O. M., Venkataramani, V., Harrison, F. E., Lee, E., Bowman, A. B., & Aschner, M. (2019). New insights on the role of manganese in Alzheimer’s Disease and Parkinson’s Disease. International Journal of Environmental Research and Public Health, 16(19), 3546. doi: 10.3390/ijerph16193546
32. MHPRC. (2013) National Food Safety Standard, maximum levels of contaminants in foods (GB2762-2012).
33. MHPRC. (2022). National Food Safety Standard, Maximum Levels of Contaminants in Foods (GB2762-2022). Erişim tarihi: 01 Haziran 2024, https://fas.usda.gov/data/china-china-releases-standard-maximum-levels-contaminants-foods-0
34. Morgano, M. A., Rabonato, L. C., Milani, R. F., Miyagusku, L., & Balian, S. C. (2011). Assessment of trace elements in fishes of Japanese foods marketed in São Paulo (Brazil). Food Control, 22(5), 778-785. doi: 10.1016/j.foodcont.2010.11.016
35. Mukaka, M. M. (2012). Statistics corner: A guide to appropriate use of correlation coefficient in medical research. Malawi Medical Journal, 24(3), 69-71.
36. Naz, S., Chatha, A. M. M., & Danabas, D. (2023). Effects of cadmium and nickel on embryonic development of fish: a review. Menba Journal of Fisheries Faculty, 9(2), 40-51. doi: 10.58626/menba.1266952
37. Orhan, M. A. (2023). “Çeşit Çok Ama Karaman Halkı Hamsi Diyor”. KGRt, Erişim tarihi: 01 Ekim 2024, https://www.kgrthaber.com/cesit-cok-ama-karaman-halki-hamsi-diyor
38. Prabakaran, K., Sompongchaiyakul, P., Bureekul, S., Wang, X., & Charoenpong, C. (2024). Heavy metal bioaccumulation and risk assessment in fishery resources from the Gulf of Thailand. Marine Pollution Bulletin, 198, 115864. doi: 10.1016/j.marpolbul.2023.115864
39. Rajeshkumar, S., & Li, X. (2018). Bioaccumulation of heavy metals in fish species from the Meiliang Bay, Taihu Lake, China. Toxicology Reports, 5, 288–295. doi: 10.1016/j.toxrep.2018.01.007
40. Sai Bhavya, K., Selvaran, A., Samrot, A. V., Javad, P. T. M., & Appalaraju, V. V. S. S. (2019). Leather processing, its effects on environment and alternatives of chrome tanning. International Journal of Advanced Research in Engineering and Technology (IJARET). 10(6), 69-79. doi: 10.34218/IJARET.10.6.2019.009
41. Qin, D., Jiang, H., Bai, S., Tang, S., & Mou, Z. (2015). Determination of 28 trace elements in three farmed cyprinid fish species from Northeast China. Food Control, 50, 1-8. doi: 10.1016/j.foodcont.2014.08.016
42. Tarley, C. R. T., Coltro, W. K. T., Matsushita, M., & de Souza, N. E. (2001). Characteristic levels of some heavy metals from Brazilian canned sardines (Sardinella brasiliensis). Journal of Food Composition and Analysis, 14, 611–617. doi: 10.1006/jfca.2001.1028
43. TGK. (2023). Türk Gıda Kodeksi Bulaşanlar Yönetmeliği. (2023, 05 Kasım) Resmi Gazete (Sayı: 32360). Erişim tarihi: 01 Haziran 2024, https://www.resmigazete.gov.tr/eskiler/2023/11/20231105-1.htm
44. TOB. (2021). Su Ürünleri İstatistikleri. Erişim tarihi: 20 Haziran 2023, https:/www.tarimorman.gov.tr/sgb/ Belgeler/Sag MenuVeriler /BSGM.pdf
45. Tokatlı, C., & Ustaoğlu, F. (2021). Meriç Delta balıklarında toksik metal birikimlerinin değerlendirmesi: muhtemel insan sağlığı riskleri. Acta Aquatica Turcica, 17(1), 136-145. doi: 10.22392/actaquatr.769656
46. Türkmen, A., Tepe, Y., & Türkmen, M. (2016). Determination of metals in tissues of fish species from Hurmabogazı Lagoon. Indian Journal of Geo-Marine Sciences, 45(2), February 2016, 277-282.
47. Türkmen, M., Türkmen, A., Tepe, Y., Töre, Y., & Ateş, A. (2009). Determination of metalsin fish species from Aegean and Mediterranean Seas. Food Chemistry, 113, 233-237. doi: 10.1016/j.foodchem.2008.06.071
48. Tuzen, M. (2009). Toxic and essential trace elemental contents in fish species from the Black Sea, Turkey. Food and Chemical Toxicology, 47(8), 1785-1790. doi: 10.1016/j.fct.2009.04.029
49. UNEP. (1984). Determination of total cadmium, zinc, lead and copper in selected marine organisms by flameless atomic absorption spectrophotometry. Reference Methods for Marine Pollution Studies No. 11 Rev. 1. Erişim tarihi: 20 Haziran 2023, https://wedocs.unep.org/20.500.11822/28494
50. USEPA. (1989) Risk Assessment Guidance For Superfund. Volume I: Human Health Evaluation Manual (Part A). İnterim Final EPA/540/1-89/002. Erişim tarihi: 20 Haziran 2023, https://www.epa.gov/sites/default/files/2015-09/documents/rags_a.pdf
51. USEPA. (1991) Risk Assessment Guidance For Superfund Volume I: Human Health Evaluation Manual, Supplemental Guidance Standard Default Exposure Factors İnterim Final OSWER Directive 9285.6-03 Erişim tarihi: 20 Haziran 2023, https://www.epa.gov/sites/default/files/2015-11/documents/OSWERdirective9285.6-03.pdf
52. USEPA. (2011) Exposure Factors Handbook 2011 edition EPA/600/R-09/052F. Erişim tarihi: 20 Haziran 2023, https://www.regulations.gov/document/EPA-HQ-OAR-2022-0730-0049
53. USEPA. (2019). Regional Screening Levels (RSLs)–Equations. Erişim tarihi: 20 Haziran 2023, https://www.epa.gov/risk/regional-screening-levels-rsls-equations
54. USEPA. (2021). Regional Screening Level (RSL) Summary Table (TR=1E-06 THQ=1.0). Erişim tarihi: 20 Haziran 2023 https://semspub.epa.gov/work/HQ/401635.pdf
55. Varol, M., & Sünbül, M. R. (2017). Comparison of heavy metal levels of farmed and escaped farmed rainbow trout and health risk assessment associated with their consumption. Environmental Science and Pollution Research, 24, 23114-23124. doi: 10.1007/s11356-017-9958-5
56. Varol, M., Kaya, G. K., & Sünbül, M. R. (2019). Evaluation of health risks from exposure to arsenic and heavy metals through consumption of ten fish species. Environmental Science and Pollution Research, 26(32), 33311-33320. doi: 10.1007/s11356-019-06450-x
57. Varol, M., Kaçar, E., & Akın, H. K. (2020). Accumulation of trace elements in muscle, gill and liver of fish species (Capoeta umbla and Luciobarbus mystaceus) in the Tigris River (Turkey), and health risk assessment. Environmental Research, 186, 109570. doi: 10.1016/j.envres.2020.109570
58. Varol, M., Kaçar, E., Sünbül, M. R., & Islam, A. R. M. T. (2022a). Levels of metals and elements in tissues of fish species in the Kızılırmak River (Turkey) and assessment of health risks and nutritional benefits. Environmental Research, 214, 113791. doi: 10.1016/j.envres.2022.113791
59. Varol, M., Kaçar, E., Sünbül, M. R., & Islam, A. R. M. T. (2022b). Species, tissue and gender-related metal and element accumulation in fish species in a large reservoir (Turkey) and health risks and nutritional benefits for consumers. Environmental Toxicology and Pharmacology, 94, 103929. doi: 10.1016/j.etap.2022.103929
60. Varol, M., & Kaçar, E. (2023). Bioaccumulation of metals in various tissues of fish species in relation to fish size and gender and health risk assessment. Current Pollution Reports, 9(3), 327-337. doi: 10.1007/s40726-023-00263-w
61. Yin, J., Wang, L., Liu, Q., Li, S., Li, J., & Zhang, X. (2020). Metal concentrations in fish from nine lakes of Anhui Province and the health risk assessment. Environmental Science and Pollution Research, 27, 20117–20124. doi: 10.1007/s11356-020-08368-1
62. Zerizghi, T., Yang, Y., Wang, W., Zhou, Y., Zhang, J., & Yi, Y. (2020). Ecological risk assessment of heavy metal concentrations in sediment and fish of a Shallow Lake: a case study of Baiyangdian Lake, North China. Environmental Monitoring and Assessment, 192(2), 154. doi: 10.1007/s10661-020-8078-8
63. Zyadah, M., & Chouikhi, A. (1999). Heavy metal accumulation in mullus barbatus, merluccius and boops fish from the Aegean Sea, Turkey. International Journal of Food Sciences and Nutrition, 50, 429–434. doi: 10.1080/09637489910099