Thiol-disulfide homeostasis as a marker of oxidative stress in soccer players was not affected by heart rate variability

Yıl 2023, Cilt: 5 Sayı: 2, 52 - 61, 31.12.2023

Yıldırım Kayacan Hami Sergen Katırcıoğlu

Öz

Objective: The study aims to examine the relationship between thiol-disulfide balance and autonomic nervous system activity in soccer players.
Methods: This study was conducted with 15 male soccer players between the ages of 19-32. ECG measurements were taken from the athletes at rest in the morning after waking up and heart rate variability (HRV) data were analyzed as an indicator of autonomic nervous system activity. Thiol disulfide homeostasis status was determined using a novel automated spectrophotometric analysis method before and after the match. The obtained findings were analyzed by using Pearson correlation and paired t-test.
Results: After the match, an increase was observed in the thiol disulfide (TD) parameters. But there was no statistically significant difference in the TD parameters before and after the match (P> 0.05). It was observed that the correlation between age and TD parameters disappeared after the exercise. No overall relationship was observed between HRV parameters and thiol-disulfide parameters (except for MINRR and AVRR parameters). A strong correlation was observed within the time and frequency-dependent HRV parameters
Conclusion: The current study examines the relationship between thiol-disulfide balance and HRV as a marker of oxidative stress for the first time. In addition, it was observed that mild-moderate exercise does not affect oxidative stress in trained athletes and works independently from the autonomic nervous system. It is considered that SDNN and RMSSD can be used as an important marker in the analysis of the parasympathetic system.

Anahtar Kelimeler

Thiol-disulfide homeostasis, HRV, Oxidative stress, Football

Destekleyen Kurum

Ondokuz Mayıs Üniversitesi

Proje Numarası

PYO.YDS.1904.18.009

Kaynakça

• Aldred S, Rohalu MA. Moderate intensity exercise program did not increase the oxidative stress in older adults. Archives of gerontology and geriatrics 2011;53(3):350-353.
• Ates I, Ozkayar N, Topcuoglu C, Dede F. Relationship between oxidative stress parameters and asymptomatic organ damage in hypertensive patients without diabetes mellitus. Scandinavian Cardiovascular Journal 2015;49(5):249-256.
• Beck ON. Use aerobic energy expenditure instead of oxygen uptake to quantify exercise intensity and predict endurance performance. Journal of applied physiology 2018;125 (2):672–674.
• Bektas, H., Vural, G., Gumusyayla, S., Deniz, O., Alisik, M., & Erel, O. (2016). Dynamic thiol–disulfide homeostasis in acute ischemic stroke patients. Acta Neurologica Belgica, 116(4), 489-494.
• Boutcher, S. H., & Stein, P. Association between heart rate variability and training response in sedentary middle-aged men. European journal of applied physiology and occupational physiology,1995; 70(1), 75-80.
• Campese VM, Ye S, Zhong H, Yanamadala V, Ye Z, Chiu J. Reactive oxygen species stimulates central and peripheral sympathetic nervous activity. Am J Physiol Heart Circ Physiol 2004; 286: 695–703.
• Chahine T, Baccarelli A, Litonjua A, Wright RO, Suh H, Gold DR, Schwartz J. Particulate air pollution, oxidative stress genes, and heart rate variability in an elderly cohort. Environmental health perspectives 2007;115(11):1617-1622.
• Chuang, H. C., Hsueh, T. W., Chang, C. C., Hwang, J. S., Chuang, K. J., Yan, Y. H., & Cheng, T. J. Nickel-regulated heart rate variability: the roles of oxidative stress and inflammation. Toxicology and applied pharmacology,2013; 266(2), 298-306.
• Cremers CM., Jakob U. Oxidant sensing by reversible disulfide bond formation. Journal of Biological Chemistry 2013;288(37):26489-26496.
• DeGiorgio CM, Miller P, Meymandi S, Chin A, Epps J, Gordon S, Harper RM. RMSSD, a measure of vagus-mediated heart rate variability, is associated with risk factors for SUDEP: the SUDEP-7 Inventory. Epilepsy & Behavior 2010;19(1),78-81.
• Düzova H, Emre MH, Karakoç Y, Karabulut AB, Yılmaz Z, Gürsul C, Yoloğlu S. The effects of moderate and strenuous running exercis on muscle and erythrocyte oxidant/antioxidant status. Journal of Sports Science and Medicine. 2008;8, 219-224
• Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clinical biochemistry 2014;47(18):326-332.
• Gorman JM, Sloan RP. Heart rate variability in depressive and anxiety disorders. American heart journal 2000;140(4):77-83.
• Hambrook, J. R., Gharamah, A. A., Pila, E. A., Hussein, S., & Hanington, P. C. Biomphalaria glabrata Granulin Increases Resistance to Schistosoma mansoni Infection in Several Biomphalaria Species and Induces the Production of Reactive Oxygen Species by Haemocytes. Genes, 2020; 11(1), 38.
• Huertas JR, Antioxidant effect of exercise: exploring the role of the mitochondrial complex I superassembly. Redox biology 2017;13: 477–481.,
• Huikuri HV, Mäkikallio TH, Perkiömäki J. Measurement of heart rate variability by methods based on nonlinear dynamics. Journal of electrocardiology 2003;36: 95-99.
• Yazar, H., Kayacan, Y., & Erel, Ö. (2022). Thiol-Disulfide Homeostasis as an Oxidative Stress Indicator: Applications to Nutrition. In Biomarkers in Nutrition (pp. 801-818). Cham: Springer International Publishing.
• Kayacan Y, Tapan T, Makaracı Y, Uçar C, Yıldız S. Salivary cortisol levels in elite male handball players during a match. Journal of Experimental and Clinical Medicine, 2017;34(3), 185-189.
• Kayacan, Y., & Yazar, H. (2022). Oxidative Stress Biomarkers in Exercise: Intake of Supplements. In Biomarkers in Nutrition (pp. 1-14). Cham: Springer International Publishing.
• Kayacan Y, Yazar H, Kisa EC, Ghojebeigloo BE. A novel biomarker explaining the role of oxidative stress in exercise and l-tyrosine supplementation: thiol/disulphide homeostasis. Archives of physiology and biochemistry 2018;124(3):232-236.
• Kayacan, Y., & Yildiz, S. Resting and postexercise heart rate variability in professional handball players. The journal of sports medicine and physical fitness,2016;56(3), 302-310.
• Laumbach, R. J., Kipen, H. M., Ko, S., Kelly-McNeil, K., Cepeda, C., Pettit, A., . & Veleeparambil, M. A controlled trial of acute effects of human exposure to traffic particles on pulmonary oxidative stress and heart rate variability. Particle and fibre toxicology, 2014;11(1), 45.
• Malik M. Heart rate variability: Standards of measurement, physiological interpretation, and clinical use: Task force of the European Society of Cardiology and the North American Society for Pacing and Electrophysiology. Annals of Noninvasive Electrocardiology 1996;1(2):151-181.
• Nandeesha H, Sathiyapriya V, Zachariah B, Pavithran P, Agrawal A, Selvaraj N. Altered oxidant-antioxidant status in non-obese men with moderate essential hypertension. Ind J Med Sci. 2007; 61: 326–331
• Niess AM. DNA damage after exhaustive treadmill running in trained and untrained men. International journal of sports medicine 1996;17(06):397–403.
• Pal S. High-intensity exercise induced oxidative stress and skeletal muscle damage in postpubertal boys and girls: a comparative study. The journal of strength & conditioning research 2018;32(4):1045–1052.
• Pavithran, P., Nandeesha, H., Sathiyapriya, V., Bobby, Z., & Madanmohan, T. Short-term heart variability and oxidative stress in newly diagnosed essential hypertension. Clinical and experimental hypertension,2008;30(7), 486-496.
• Pingitore A. Exercise and oxidative stress: potential effects of antioxidant dietary strategies in sports. Nutrition 2015;31(7–8):916–922.
• Ravi Kiran T, Subramanyam Mv, Asha Devi S. Swim exercise training and adaptations in the antioxidant defense system of myocardium of old rats: relationship to swim intensity and duration. Comp Biochem Physiol B Biochem Mol Biol. 2004;137(2):187-96.
• Sathiyapriya V, Selvaraj N, Nandeesha H, Zachariah B, Agrawal A, Pavithran P. Increased protein glycation in non-diabetic essential hypertension: Role of lipid peroxides. Arch Med Res. 2007; 38: 822–826
• Schafer FQ, Buettner GR. Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple. Free radical biology and medicine 2001;30(11):1191-1212.
• Schneider CD, Barp J, Ribeiro JL, Klein BA, Oliveira AR. Oxidative stress after three different intensities of running. Can. J. Appl. Physiol. 2005;30 (6): 723-734.
• Sen CK, Packer L. Thiol homeostasis and supplements in physical exercise. The American journal of clinical nutrition 2000;72(2): 653-669.
• Takimoto E, Kass DA. Role of oxidative stress in cardiac hypertrophy and remodeling. Hypertension. 2007; 49: 241–248
• Wessendorf R. L., & Lu, Y. Photosynthetic characterization of transgenic Synechocystis expressing a plant thiol/disulfide-modulating protein. Plant Signaling & Behavior, 2020;1709708.
• Windham BG, Fumagalli S, Ble A, Sollers JJ, Thayer JF, Najjar SS, Ferrucci L. The relationship between heart rate variability and adiposity differs for central and overall adiposity. Journal of obesity 2012(149516);2012:8.