Effects of penicillin and montelukast sodium on middle ear mucosa in rats with experimental acute otitis media

Abstract

Objective: To develop an experimental acute suppurative otitis media model and compare the responses of rats to penicillin and combinations of leukotriene antagonist with respect to histopathological observations conducted at both early and late phases. Methods: A total of eighty-three ears from fifty six Wistar rats used in this study. Pneumococcus suspension was injected transtympanically to all the rats. Subjects were classified under four different groups having 14 rats at each. In Group A, intramuscular penicillin G was injected to rats for a period of five days. In Group B, intraperitoneal montelukast was injected for 21 days in addition to penicillin. In Group C, intraperitoneal montelukast and in Group D intraperitoneal isotonic NaCl was injected to rats for 21 days. Cross-sections were semi-quantitatively graded with respect to various inflammatory components. Results: No significant difference was found between the groups, apart from mucosal vascularization with respect to mucosal and tympanic membrane (TM) parameters at early phases. However, statistically significant differences were found for the improvement of TM thickness with the help of penicillin treatment. Furthermore, considerable deviations were observed for the recuperation of TM and mucosal inflammation for groups where subjects were injected with montekulast as compared to other groups of the study. Conclusion: The results of this study clearly show that the beneficial effects of the antibiotic (penicillin) as well as leukotriene antagonist (montelukast) is statistically different those of placebo in acute otitis media in rats. When the parameters of inflammation in the rat middle ear were compared with each other, most of these parameters did not show any statistically different beneficial effects in montelukast and penicillin groups.

Keywords

• Acute otitis media, leukotriene, montelukast, tympanic mucosa

Atomik kuvvet mikroskobu ve tarayıcı elektron mikroskobuyla timpanosklerotik plakların analizi

Öz

Amaç: Atomik kuvvet mikroskobu ile kalsiyum kürecikleri oluşturan timpanosklerotik plak oluşumunu görüntüleyerek literatüre yeni bilgiler sunmak ve tarayıcı elektron mikroskobuna monte edilmiş enerji yayıcı X-ışını dedektörü ile temel yapısını incelemek. Yöntem: Timpanoskleroz cerrahisi geçirmiş 30 hastadan alınan örnekler üçüncü basamak sevk merkezimizde geriye dönük olarak incelendi. Atomik kuvvet mikroskobuyla en sert plağın yüzey topografisi ve üç boyutlu görüntüleri analiz edildi ve tarayıcı elektron mikroskobu – enerji yayıcı X-ışını spektroskopisiyle 5 farklı plak içindeki 5 farklı kalsiyum küreciğinin temel bileşimi incelendi. Bulgular: Atomik kuvvet mikroskobuyla timpanosklerotik plağın üç boyutlu analizi kalsiyum fosfat kristalli oluşumları göstermiştir. Var olan kalsiyum, fosfat, karbon, nitrojen, oksijen, sodyum ve magnezyum miktarının tayini için enerji yayıcı X-ışını spektroskopisiyle tarayıcı elektron mikroskobu kullanılmıştır. Sonuç: Timpanosklerotik plağın temel bileşimi hakkında bilgi edinmek için yüzey topografisi kullanımının timpanosklerozun etiyolojisi ve tedavisini anlayışımıza katkıda bulunacağına inanmaktayız.

Anahtar Kelimeler

• Timpanoskleroz, plak, analiz

Kaynakça

1. Gibb AG. Tympanosclerosis. Proc Roy Soc Med 1976;69:155- 62.
2. Friedmann I, Galey FR. The initiation and stages of mineraliza- tion in tympanosclerosis. J Laryngol Otol 1980;94:1215-29.
3. Zöllner F, Beck C. Tympanosclerosis. Zeitschrift für Laryngologie und Rhinologie 1955;34:137-55.
4. Goldstein J, Newbury DE, Joy DC, et al. Scanning Electron Microscopy and X-Ray Microanalysis. 3rd ed. New York: Springer; 2003. p. 127-133.
5. Unertl WN. The surface structure of crystalline solids. In: Bonnell D, editor. Scanning Probe Microscopy and Spectroscopy. Theory, Techniques, and Applications. 2nd ed. New York: Wiley; 2001:115-149.
6. Schiff M, Poliquin JF, Catanzaro A, et al. Tympanosclerosis. A the- ory of pathogenesis. Ann Otol Rhinol Laryngol 1980;89(Suppl.):1- 16.
7. Friedman I, Galey R, Odnert S. The ultrastructure of tym- panosclerosis: the source of the matrix vesicles and the pattern of calcospherules. Am J Otol 1981;3:144-9.
8. Bonucci E. The locus of initial calcification in cartilage and bone. Clin Orthop Relat Res 1971;(78):108-9.

9. Bonucci E. Fine structure and histochemistry of calcifying glob- ules in epiphyseal cartilage. Z Zellforsch Mikrosk Anat 1970; 103:192-217.
10. McKee GJ, Kerr AG. Tympanosclerosis: a scanning electron microscopic study. Clin Otolaryngol Allied Sci 1989;14:11-6.
11. Buyanover D, Tietz A, Luntz M, Sadé J. The biochemical com- position of tympanosclerotic deposits. Arch Otorhinolaryngol 1987;243:366-9.
12. Doner F, Yariktas M, Dogru H, Uzun H, Aydin S, Delibas N. The biochemical analysis of tympanosclerotic plaques. Otolaryngol Head Neck Surg 2003;128:742-5.
13. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY
14. NC-ND3.0) Licence (http://creativecommons.org/licenses/by-nc-nd/3.0/) which permits unrestricted noncommercial use, distribution, and reproduc
15. tion in any medium, provided the original work is properly cited.
16. Please cite this article as: Aslan H, Başoğlu MS, İlknur AE, Özbay C, Eren E, Oğuz Kılıç D, Kulduk E, Öztürkcan S, Katılmış H. Analysis of tym
17. panosclerotic plaques via atomic force microscope and scanning electron microscope. J Med Updates 2012;2(3):89-93.