Araştırma Makalesi
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3D Printed Polylactic Acid Scaffold For Dermal Tissue Engineering Application: The Fibroblast Proliferation in Vitro

Yıl 2019, Cilt: 1 Sayı: 2, 51 - 56, 16.12.2019

Öz

Dermal fibroblasts are mesenchymal cells that produce extracellular matrix. Fibroblasts play an important role in the skin wound healing process and skin bioengineering. The aim of this study is to evaluate the behaviour of 3D printed polylactic acid (PLA) scaffolds in terms of biocompatibility and toxicity on human dermal fibroblasts (HDFs). Scaffolds were prepared with the PLA filament using a custom made fused deposition modeling (FDM) printer. We fabricated scaffolds with two different pore sizes (35% and 40%). HDFs were seeded at different densities on PLA scaffolds. The cell growth was measured by WST-1 colorimetric assay after 12 and 18 days of seeding HDFs on 3D PLA scaffolds. The morphology and the adhesion property of HDFs were visualized by scanning electron microscopy (SEM). HDFs showed a significant cell proliferation in 3D printd PLA scaffolds. The cell proliferation was highest at a density of 4 x 104 cells per well. SEM images showed that HDFs attached the surfaces of the scaffolds and filled the inter-fiber gaps. Our results showed that PLA scaffolds fabricated by 3D bioprinting is a promising candidate for HDF seeding and could have a potential application wound healing or personalized drug trials.

Kaynakça

  • 1. Darby IA and Hewitson TD. 2007. Fibroblast differentiation in wound healing and fibrosis. International Review of Cytology. 257: 143-79.
  • 2. Takahashi‐Iwanaga H. 1991. The three‐dimensional cytoarchitecture of the interstitial tissue in the rat kidney. Cell and Tissue Research. 264: 269-281.
  • 3. Grinnell F, Ho CH, Tamariz E, Lee DJ, Skuta G. 2003. Dendritic Fibroblasts in Three-dimensional Collagen Matrices. Molecular Biology of the Cell. 14: 384-395
  • 4. Darby IA, Laverdet B, Bonté F, Desmoulière A. 2014. Fibroblasts and myofibroblasts in wound healing. Clinical, Cosmetic and Investigational Dermatology. 7: 301-311.
  • 5. Rozario T, DeSimone DW. 2010. The extracellular matrix in development and morphogenesis: a dynamic view. Developmental Biology. 341: 126-140.
  • 6. Hutmacher DW, Sittinger M, Risbud MV. 2004. Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems. Trends Biotechnology. 22: 354-62.
  • 7. Chanjuan D, Yonggang LV. 2016. Application of collagen scaffold in tissue engineering: recent advances and new perspectives. Polymers. 8: 2-42
  • 8. Guntillake PA, Adhikari R. 2003. Biodegradable synthetic polymers for tissue engineering. European Cells & Materials. 5: 1-16.
  • 9. Cui M, Liu L, Guo N, Su R, Ma F. 2015. Preparation, cell compatibility and degradability of collagen-Modified poly(lactic acid). Molecules. 20: 595-607.
  • 10. Akbarzadeh R, Yousefi AM. 2014. Effects of processing parameters in thermally induced phase separation technique on porous architecture of scaffolds for bone tissue engineering. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 102(6): 1304-15. 11. Dizon JRC, Espera AH, Chen Q, Advincula RC. 2018. Mechanical characterization of 3D-printed polymers. Additive Manufacturing. 20: 44-67.
  • 12. Bracaglia LG, Smith BT, Watson E, Arumugasaamy N, Mikos AG, Fisher JP. 2017. 3D Printing for the design and fabrication of polymer-based gradient scaffolds. Acta Biomaterialia. 56: 3
  • 13. Li Q, Li L, Li Z, Gong F, Feng W, Jiang X, Xiong P. 2002. Antitumor effects of the fibroblasts transfected TNF-alpha gene and its mutants. Journal of Huazhong University of Science and Technology Medical Sciences. 22: 92-95.
  • 14. Mohiti-Asli M, Saha S, Murphy SV, Gracz H, Pourdeyhimi B, Atala A, Loboa EG. 2015. Ibuprofen loaded PLA nanofibrous scaffolds increase proliferation of human skin cells in vitro and promote healing of full thickness incision wounds in vivo. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 105(2): 327-339.
  • 15. Gregor A, Filová E, Novák M, Kronek J, Chlup H, Buzgo M, Blahnová V, Lukášová V, Bartoš M, Nečas A, Hošek J. 2017. Designing of PLA scaffolds for bone tissue replacement fabricated by ordinary commercial 3D printer. Journal of Biological Engineering. 11: 31.

Dermal Doku Mühendisliği Uygulaması için 3B Baskılı Doku İskelesi: In vitro Fibroblast Proliferasyonu

Yıl 2019, Cilt: 1 Sayı: 2, 51 - 56, 16.12.2019

Öz

Fibroblastlar ekstrasellüler matriks üreten mezenkimal hücrelerdir. Fibroblastlar deride yara iyileşme sürecinde ve deri biyomühendisliğinde önemli bir role sahiptir. Bu çalışmanın amacı 3B baskılı Polilaktik asit (PLA) iskelelerinin insan dermal fibroblastlar (HDF) üzerindeki biyouyumluluk ve toksisite etkilerinin değerlendirilmesidir. İskeleler PLA malzemesi ile özel tasarım birleştirmeli yığma modellemesi (BYM) 3B yazıcı kullanılarak hazırlanmıştır. Çalışma için iki farklı por boyutunda (%35 ve %40) PLA iskeleler üretilmiştir. HDF’ler PLA iskelelere farklı sayılarda ekilmiştir. 3B PLA iskelelere ekilen HDF’lerin hücre çoğalması çalışmanın 12. ve 18. gününde WST-1 kolorimetrik yöntemi ile ölçülmüştür. HDF morfolojisi ve adhezyon özellikleri taramalı elektron mikroskopisi (SEM) ile görselleştirilmiştir. En yüksek hücre yoğunluğu 4 x 104 hücre ekilen grupta ölçülmüştür. SEM görüntüleri HDF’lerin iskele yüzeylerine tutunduklarını ve fiber-arası boşlukları doldurduklarını göstermiştir. Sonuçlarımız 3B baskılı üretilen PLA iskelelerin HDF ekiminde kullanılabileceğini ve yara iyileşmesi ve kişiselleştirilmiş ilaç denemelerinde potansiyel bir uygulamaya aday teşkil ettiklerini göstermiştir.

Kaynakça

  • 1. Darby IA and Hewitson TD. 2007. Fibroblast differentiation in wound healing and fibrosis. International Review of Cytology. 257: 143-79.
  • 2. Takahashi‐Iwanaga H. 1991. The three‐dimensional cytoarchitecture of the interstitial tissue in the rat kidney. Cell and Tissue Research. 264: 269-281.
  • 3. Grinnell F, Ho CH, Tamariz E, Lee DJ, Skuta G. 2003. Dendritic Fibroblasts in Three-dimensional Collagen Matrices. Molecular Biology of the Cell. 14: 384-395
  • 4. Darby IA, Laverdet B, Bonté F, Desmoulière A. 2014. Fibroblasts and myofibroblasts in wound healing. Clinical, Cosmetic and Investigational Dermatology. 7: 301-311.
  • 5. Rozario T, DeSimone DW. 2010. The extracellular matrix in development and morphogenesis: a dynamic view. Developmental Biology. 341: 126-140.
  • 6. Hutmacher DW, Sittinger M, Risbud MV. 2004. Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems. Trends Biotechnology. 22: 354-62.
  • 7. Chanjuan D, Yonggang LV. 2016. Application of collagen scaffold in tissue engineering: recent advances and new perspectives. Polymers. 8: 2-42
  • 8. Guntillake PA, Adhikari R. 2003. Biodegradable synthetic polymers for tissue engineering. European Cells & Materials. 5: 1-16.
  • 9. Cui M, Liu L, Guo N, Su R, Ma F. 2015. Preparation, cell compatibility and degradability of collagen-Modified poly(lactic acid). Molecules. 20: 595-607.
  • 10. Akbarzadeh R, Yousefi AM. 2014. Effects of processing parameters in thermally induced phase separation technique on porous architecture of scaffolds for bone tissue engineering. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 102(6): 1304-15. 11. Dizon JRC, Espera AH, Chen Q, Advincula RC. 2018. Mechanical characterization of 3D-printed polymers. Additive Manufacturing. 20: 44-67.
  • 12. Bracaglia LG, Smith BT, Watson E, Arumugasaamy N, Mikos AG, Fisher JP. 2017. 3D Printing for the design and fabrication of polymer-based gradient scaffolds. Acta Biomaterialia. 56: 3
  • 13. Li Q, Li L, Li Z, Gong F, Feng W, Jiang X, Xiong P. 2002. Antitumor effects of the fibroblasts transfected TNF-alpha gene and its mutants. Journal of Huazhong University of Science and Technology Medical Sciences. 22: 92-95.
  • 14. Mohiti-Asli M, Saha S, Murphy SV, Gracz H, Pourdeyhimi B, Atala A, Loboa EG. 2015. Ibuprofen loaded PLA nanofibrous scaffolds increase proliferation of human skin cells in vitro and promote healing of full thickness incision wounds in vivo. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 105(2): 327-339.
  • 15. Gregor A, Filová E, Novák M, Kronek J, Chlup H, Buzgo M, Blahnová V, Lukášová V, Bartoš M, Nečas A, Hošek J. 2017. Designing of PLA scaffolds for bone tissue replacement fabricated by ordinary commercial 3D printer. Journal of Biological Engineering. 11: 31.
Toplam 14 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Cerrahi
Bölüm Araştırma Makaleleri
Yazarlar

Ufkay Karabay 0000-0001-8608-1865

R. Buğra Hüsemoğlu Bu kişi benim 0000-0003-1979-160X

Mehtap Yüksel Eğrilmez Bu kişi benim 0000-0002-3570-1865

Hasan Havıtçıoğlu Bu kişi benim 0000-0001-8169-3539

Yayımlanma Tarihi 16 Aralık 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 1 Sayı: 2

Kaynak Göster

APA Karabay, U., Hüsemoğlu, R. B., Yüksel Eğrilmez, M., Havıtçıoğlu, H. (2019). 3D Printed Polylactic Acid Scaffold For Dermal Tissue Engineering Application: The Fibroblast Proliferation in Vitro. Journal of Medical Innovation and Technology, 1(2), 51-56.
AMA Karabay U, Hüsemoğlu RB, Yüksel Eğrilmez M, Havıtçıoğlu H. 3D Printed Polylactic Acid Scaffold For Dermal Tissue Engineering Application: The Fibroblast Proliferation in Vitro. Journal of Medical Innovation and Technology. Aralık 2019;1(2):51-56.
Chicago Karabay, Ufkay, R. Buğra Hüsemoğlu, Mehtap Yüksel Eğrilmez, ve Hasan Havıtçıoğlu. “3D Printed Polylactic Acid Scaffold For Dermal Tissue Engineering Application: The Fibroblast Proliferation in Vitro”. Journal of Medical Innovation and Technology 1, sy. 2 (Aralık 2019): 51-56.
EndNote Karabay U, Hüsemoğlu RB, Yüksel Eğrilmez M, Havıtçıoğlu H (01 Aralık 2019) 3D Printed Polylactic Acid Scaffold For Dermal Tissue Engineering Application: The Fibroblast Proliferation in Vitro. Journal of Medical Innovation and Technology 1 2 51–56.
IEEE U. Karabay, R. B. Hüsemoğlu, M. Yüksel Eğrilmez, ve H. Havıtçıoğlu, “3D Printed Polylactic Acid Scaffold For Dermal Tissue Engineering Application: The Fibroblast Proliferation in Vitro”, Journal of Medical Innovation and Technology, c. 1, sy. 2, ss. 51–56, 2019.
ISNAD Karabay, Ufkay vd. “3D Printed Polylactic Acid Scaffold For Dermal Tissue Engineering Application: The Fibroblast Proliferation in Vitro”. Journal of Medical Innovation and Technology 1/2 (Aralık 2019), 51-56.
JAMA Karabay U, Hüsemoğlu RB, Yüksel Eğrilmez M, Havıtçıoğlu H. 3D Printed Polylactic Acid Scaffold For Dermal Tissue Engineering Application: The Fibroblast Proliferation in Vitro. Journal of Medical Innovation and Technology. 2019;1:51–56.
MLA Karabay, Ufkay vd. “3D Printed Polylactic Acid Scaffold For Dermal Tissue Engineering Application: The Fibroblast Proliferation in Vitro”. Journal of Medical Innovation and Technology, c. 1, sy. 2, 2019, ss. 51-56.
Vancouver Karabay U, Hüsemoğlu RB, Yüksel Eğrilmez M, Havıtçıoğlu H. 3D Printed Polylactic Acid Scaffold For Dermal Tissue Engineering Application: The Fibroblast Proliferation in Vitro. Journal of Medical Innovation and Technology. 2019;1(2):51-6.