Use and comparison of MTT, XTT and iCELLigence methods in the evaluation of drug toxicity

Yıl 2025, Cilt: 6 Sayı: 1, 66 - 71, 14.02.2025

Ayşe Nur Coşkun Demirkalp

https://doi.org/10.47582/jompac.1605042

Öz

Toxicology tests are one of the fundamental methods used in biological and pharmaceutical research. These tests are used to evaluate the viability, proliferation and toxic responses of cells. The study of biological activities of cells plays a critical role in drug development, cancer research, toxicology and various biotechnological applications. Drug toxicology is an important field of research to determine the harmful effects of drugs on cellular and biological systems. In vitro tests are widely used for accurate evaluation of drugs and their toxic effects. These tests examine the effects of drugs on cell cultures, rapidly revealing their potential harmful effects in terms of time and resources. This review discusses the advantages of the MTT (3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide) and XTT (sodium 3'-[1-(phenylamino)-carbonyl]-3,4-tetrazolium]-bis (4 methoxy-6-nitro) benzene-sulfonic acid hydrate) tests, which are widely used as cytotoxicity tests, as well as the newer method iCELLigence. MTT and XTT are widely used and reliable tests that measure cell metabolism; both methods are very effective in assessing cell viability, but provide limited dynamic data. In contrast, iCELLigence is a newer technology and provides more in-depth data by monitoring the real-time responses of cells. iCELLigence continuously monitors the growth rate and morphological changes of cells, allowing for more comprehensive and sensitive results compared to traditional methods. Comparison of these methods allows determining which methodology provides more appropriate results according to different research needs.These tests are also used to define the concentration range over which more comprehensive and detailed in vitro testing can be performed to obtain meaningful data on parameters such as genotoxicity, mutation induction or programmed cell death. This review aims to compare these three methods and discuss their advantages and limitations in the assessment of drug toxicity

Anahtar Kelimeler

iCELLigence, MTT, XTT, drug toxicity, cytotoxic

Etik Beyan

This study is a compilation and is considered a research that does not require ethics committee approval. Referee Evaluation Process Externally peer-reviewed. Conflict of Interest Statement The authors have no conflicts of interest to declare.

Destekleyen Kurum

Financial Disclosure: The authors declared that this study has received no financial support.

İlaç toksisitesinin değerlendirilmesinde MTT, XTT ve iCELLigence yöntemlerinin kullanımı ve karşılaştırılması

Öz

Toksikoloji testleri biyolojik ve farmasötik araştırmalarda kullanılan temel yöntemlerden biridir. Bu testler hücrelerin canlılığını, çoğalmasını ve toksik tepkilerini değerlendirmek için kullanılır. Hücrelerin biyolojik aktivitelerinin incelenmesi ilaç geliştirme, kanser araştırması, toksikoloji ve çeşitli biyoteknolojik uygulamalarda kritik bir rol oynar. İlaç toksikolojisi, ilaçların hücresel ve biyolojik sistemler üzerindeki zararlı etkilerini belirlemek için önemli bir araştırma alanıdır. İn vitro testler ilaçların ve toksik etkilerinin doğru değerlendirilmesi için yaygın olarak kullanılır. Bu testler ilaçların hücre kültürleri üzerindeki etkilerini inceler ve zaman ve kaynak açısından potansiyel zararlı etkilerini hızla ortaya çıkarır. MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromür), XTT (sodyum 3'-[1-(fenilamino)-karbonil]-3,4-tetrazolium]-bis(4 metoksi-6-nitro)benzen-sülfonik asit hidrat) ve iCELLigence gibi hücresel canlılık testleri ilaç toksisitesini belirlemede kullanılan yaygın yöntemlerdir. Bu testler ayrıca genotoksisite, mutasyon indüksiyonu veya programlanmış hücre ölümü gibi parametreler hakkında anlamlı veriler elde etmek için daha kapsamlı ve ayrıntılı in vitro testlerin yapılabileceği konsantrasyon aralığını tanımlamak için kullanılır. Bu derleme, bu üç yöntemi karşılaştırmayı ve ilaç toksisitesinin değerlendirilmesindeki avantajlarını ve sınırlamalarını tartışmayı amaçlamaktadır.

Anahtar Kelimeler

iCELLigence, MTT, XTT, İlaç toksisitesi, sitotoksisite

Kaynakça

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