Investigation of the effects of atmospheric pressure cold plasma on bacterial isolates

Öz

Background: Atmospheric pressure cold plasma is a type of non-thermal plasma used for antimicrobial activity in the health, food and agriculture sectors. This study was carried out to investigate the efficacy of atmospheric pressure cold plasma on different bacterial isolates. Materials and Methods: The study was conducted on standard reference bacterial strains; Escherichia coli (O157:H7), Bacillus subtilis (ATCC 6633), Enterobacter aerogenes (ATCC 13048), Rhodococcus equi (ATCC 6939), Salmonella typhimurium (ATCC 14028), Enterococcus feacalis (ATCC 2912) and field isolates; 12 Escherichia coli isolates, 22 Staphylococcus spp, 18 Klebsiella spp., 2 Enterococcus spp., 3 Acinetobacter spp., 8 Candida spp., 1 Morgarella mongarii, 2 Corynebacterium spp., 1 Streptococcus pyogenes. Atmospheric pressure cold plasma jet and plasma activated medium (PAM) were applied to the isolates and their efficacy was investigated. Results: As a result of the study, it was found that cold plasma was effective against Escherichia coli (O157:H7), Enterobacter aerogenes (ATCC 13048), Salmonella typhimurium, Rhodococcus equi (ATCC 6939) and Enterococcus feacalis (ATCC 2912) among standard bacterial strains, while it was effective against Streptococcus pyogenes (group A Bet) and Staphylococcus epidermidis among field isolates. Conclusions: As a result of our study, the antimicrobial activity of atmospheric pressure cold plasma and PAM was demonstrated, and we believe that it will contribute to the health, food and agriculture sectors.

Anahtar Kelimeler

• Atmospheric Pressure Cold Plasma
• Plasma Activated Media
• Bacterial Isolate
• Microbial Activity

Atmosferik basınç soğuk plazmanın bakteri izolatları üzerine etkilerinin araştırılması

Öz

Amaç: Atmosferik basınç soğuk plazma sağlık, gıda ve tarım sektöründe antimikrobiyal etkinliği için kullanılan termal olmayan plazma türüdür. Bu çalışma farklı bakteri izolatları üzerine atmosferik basınç soğuk plazmanın etkinliğinin araştırılması amacıyla gerçekleştirildi. Materyal ve Metot: Çalışma standart referans bakteri suşu olan; Escherichia coli (O157:H7), Bacillus subtilis (ATCC 6633), Enterobacter aerogenes (ATCC 13048), Rhodococcus equi (ATCC 6939), Salmonella typhimurium (ATCC 14028), Enterococcus feacalis (ATCC 2912) ve saha izolatı olan 12 adet Escherichia coli izolatı, 22 adet Staphylococcus spp., 18 adet Klebsiella spp., 2 adet Enterococcus spp., 3 adet Acinetobacter spp., 8 adet Candida spp., 1 adet Morgarella mongarii, 2 adet Corynebacterium spp., 1 adet Streptococcus pyogenes üzerine gerçekleştirildi. İzolatlara atmosferik basınç soğuk plazma jeti ve plazma ile aktive edilmiş ortam (PAM) uygulandı ve etkinliği araştırıldı. Bulgular: Çalışma sonucunda standart bakteri suşlarında Escherichia coli (O157:H7), Enterobacter aerogenes (ATCC 13048), Salmonella typhimurium, Rhodococcus equi (ATCC 6939) ve Enterococcus feacalis (ATCC 2912)’e soğuk plazmanın etki ettiği, saha izolatlarından ise Streptococcus pyogenes (A grubuBet) ile Staphylococcus epidermidis’e etki gösterdiği bulundu. Sonuç: Çalışmamız sonucunda atmosferik basınç soğuk plazmanın ve PAM’ın antimikrobiyal etkinliği ortaya konuldu ve bu yönüyle sağlık, gıda ve tarım sektörlerine katkı sağlayacağını düşünmekteyiz.

Anahtar Kelimeler

• Atmosferik Basınç Soğuk Plazma
• Plazma ile Aktive Edilmiş Ortam
• Bakteri İzolatı
• Mikrobiyal Etkinlik

Kaynakça

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