Thlaspi perfoliatum'un MCF-7 HÜCRELERİ VE Allium TESTİ İLE ANTİKLASTOJENİK POTANSİYELİ

Öz

Bu çalışmada, Thlaspi perfoliatum metanol özütünün insan meme kanseri hücre hattı (MCF-7) üzerindeki sitotoksik ve antiproliferatif etkileri ile toksik maddeye (CuSO₄) karşı Allium cepa kök hücreleri üzerindeki genotoksik ve mitotik onarım etkileri ayrıntılı olarak değerlendirilmiştir. MTT deneyi sonuçları, özütün MCF-7 hücrelerinin canlılığını önemli ölçüde azalttığını ve özellikle orta ve yüksek konsantrasyonlarda (400-1000 ppm) hücre çoğalmasını engellediğini ortaya koymuştur. 400-600 ppm arasında değişen LD₅₀ değeri, özütün kanser hücreleri üzerinde güçlü bir antiproliferatif etkiye sahip olduğunu göstermektedir. Öte yandan, A. cepa modeli üzerinde yapılan analizler, CuSO₄ kaynaklı mitotik indeksteki azalmanın düşük ve orta dozlarda önemli ölçüde düzeldiğini, kromozomal anormalliklerin ise doza ve uygulama zamanına bağlı olarak azaldığını ortaya koymuştur. Bu bulgular T. perfoliatum'un antikanser etkiler gösterdiğini ve biyoaktif bileşenleri sayesinde genetik materyali koruyarak hücresel onarım süreçlerini desteklediğini göstermektedir. Bu bağlamda, T. perfoliatum metanol özütü gelecekte doğal bir terapötik ajan olarak değerlendirilme potansiyeline sahiptir ve geleneksel kullanımlarının bilimsel temellerle desteklenmesine katkıda bulunmaktadır.

Anahtar Kelimeler

• Kromozom
• MCF-7
• Mitotik İndeks
• Metanol
• Thlaspi perfoliatum

ANTICLASTOGENIC POTENTIAL OF Thlaspi perfoliatum WITH MCF-7 CELLS AND Allium TEST

Öz

In this study, the cytotoxic and antiproliferative effects of Thlaspi perfoliatum methanol extract on human breast cancer cell line (MCF-7) and its genotoxic and mitotic repair effects on Allium cepa root cells against toxic substance (CuSO₄) were evaluated in detail. MTT assay results revealed that the extract significantly reduced the viability of MCF-7 cells and inhibited cell proliferation, particularly at medium and high concentrations (400–1000 ppm). The LD₅₀ value ranging between 400–600 ppm indicates that the extract has a strong antiproliferative effect on cancer cells. On the other hand, analyses conducted on the A. cepa model revealed that the CuSO₄-induced decrease in the mitotic index recovered significantly at low and medium doses, while chromosomal abnormalities decreased depending on the dose and application time. These findings demonstrate that T. perfoliatum exhibits anticancer effects and supports cellular repair processes by protecting genetic material thanks to its bioactive components. In this context, T. perfoliatum methanol extract has the potential to be evaluated as a natural therapeutic agent in the future and contributes to supporting its traditional uses with scientific foundations

Anahtar Kelimeler

• Chromosome
• MCF-7
• Mitotic Index
• Methanol
• Thlaspi perfoliatum

Kaynakça

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