The Effect of Salinity (NaCl) Stress and Different Magnetic Applications on The Germination of Cucumber Seeds (Cucumis sativus L.)

Öz

Tuzluluk stresi, tohum çimlenmesini ve bitki büyümesini sınırlayan ana faktörlerden biridir. Bu nedenle tohum çimlenmesine olumlu etkisi olduğu için manyetik su kullanılmıştır. Bu çalışmada, tuzluluk (NaCl) Stresi ve farklı manyetik uygulamaların hıyar (Cucumis sativus L.) tohumlarının çimlenmesi üzerine etkisi belirlemek amacıyla yapılmıştır. Çalışma tesadüf parselleri deneme desenine göre üç tekerrürlü yürütülmüştür. Bu çalışma, beş farklı manyetik uygulama (MT1, MT2, MT3, MT4 ve MT5) manyetik ortamdan sırasıyla 1, 2, 3, 5 ve 8 defa geçirilmiş su ve manyetik olmayan (NMT) uygulamasının hıyar tohumlarının (Cucumis sativus L.) tuzluluk stresi (S1 = 0.38 dSm-1, S2 = 4.0 dSm-1, S3 = 8.0 dSm-1) altında çimlenmesine etkisini araştırmak amacıyla yapılmıştır. Çalışma sonunda, su tuzluluğu stresindeki artış son çimlenme yüzdesi (FGP), çimlenme yüzdesi 4. gün (GP), çimlenme indeksi (GI), çimlenme hızı katsayısı (CVG) ve çimlenme oranı indeksinde (GRI) azalmaya neden olduğu göstermiştir. Bununla beraber ortalama çimlenme süresinde (MGT) artış görülmüştür. FGP, GP, GI, CVG ve GRI'nin en yüksek değerleri S1'de iken, S3'te (8.0 dSm-1) S1'e (0.38 dSm-1) göre sırasıyla %7.8, %10.0, %15.8, %2.4 ve %16.2 azalmıştır. Manyetik olan sulama suyu kullanıldığında FGP, GP, GI, CVG ve GRI'inde bir artışa neden olmuş ve manyetik olmayan sulama suyunun NMT olması durumunda ise azalmalar olduğu görülmüştür. En yüksek FGP değerleri %92.20 S1 tuzluluk (0.38 dSm-1) ile MT4 konusunda, en düşük FGP değeri %72.20 su tuzluluğu S3 (8.0 dSm-1) ile NMT konusunda belirlenmiştir. Araştırma sonuçları, manyetik su uygulamasının hıyar tohumlarının çimlenmesini olumlu etkilediğini ve tuzluluk stresinin tohumların çimlenmesini azalttığını göstermiştir. Çalışma sonucuna göre, hıyar tohumlarının en iyi çimlenmesini sağlamak için MT4 (manyetikten 5 defa geçirilmiş su) uygulanması önerilmiştir.

Anahtar Kelimeler

• Manyetik su arıtma,
• Çimlenme indeksi,
• Tuzluluk stresi,
• Hıyar,
• Tohumların çimlenmesi

The Effect of Salinity (NaCl) Stress and Different Magnetic Applications on The Germination of Cucumber Seeds (Cucumis sativus L.)

Öz

Salinity stress is one of the main factors that limit seed germination and plant growth. Therefore, magnetic water was used because it had a positive effect on seed germination. This study was conducted to investigate the effect of magnetic (MT1, MT2, MT3, MT4, and MT5) treatment of water passing through magnets 1, 2, 3, 5, and 8 times respectively, and non-magnetic (NMT) treatment on the germination of cucumber seeds (Cucumis sativus L.) under salinity stress (NaCl) (S1 = 0.38 dSm-1, S2 = 4.0 dSm-1, S3 = 8.0 dSm-1). The experiment was conducted by the design of a one-way ANOVA with three replications. The results showed that water salinity stress a significantly decrease in the final germination percentage (FGP), germination index (GI), coefficient of the velocity of germination (CVG), germination percentage 4th day (GP), and germination rate index (GRI), while the mean germination time (MGT) increased significantly. In addition, the FGP, GP, GI, CVG, and GRI were significantly increased and the MGT was significantly decreased when using magnetic water compared to non-magnetic water. The highest values of FGP, GP, GI, CVG, and GRI were at S1 while they decreased by 7.8%, 10.0%, 15.8%, 2.4% and 16.2%, respectively in S3 (8.0 dSm-1), compared to S1 (0.38 dSm-1). The highest FGP values of 92.20% at MT4 were obtained with S1 salinity (0.38 dSm-1) and the lowest FGP value of 72.20% at NMT with water salinity S3 (8.0 dSm-1). The results of the research showed that magnetic water treatment has a positive effect on the germination of cucumber seeds and that salinity stress reduced the germination of seeds. Finally, it can be recommended to apply MT4 (water passed through the magnetic 5 times) to achieve the best germination of cucumber seeds.

Anahtar Kelimeler

• Magnetic water treatment
• Germination index
• salinity stress
• Cucumber
• Germination of seeds

Kaynakça

1. Ahamed M, Elzaawely A, Bayoumi YJAJoCS (2013) Effect of magnetic field on seed germination, growth and yield of sweet pepper (Capsicum annuum L.). 5 (3):286-294
2. Akhtar P, Hussain FJPJB (2009) Growth performance of Vicia sativa L. under saline conditions. 41 (6):3075-3080
3. Aladjadjiyan AJJoCEA (2002) Study of the influence of magnetic field on some biological characteristics of Zea mais. 3 (2):89-94
4. Alsuvaid, M., Demir, Y., Kiremit, M. S., & Arslan, H. (2022). Interaction Effect of Water Magnetization and Water Salinity on Yield, Water Productivity and Morpho-Physiological of Balkız Bean (Phaseolus vulgaris). Gesunde Pflanzen, 1-16.
5. Amiri M, Dadkhah AAJC, Physicochemical SA, Aspects E (2006) On reduction in the surface tension of water due to magnetic treatment. 278 (1-3):252-255
6. Asci OOJAJoB (2011) Salt tolerance in red clover (Trifolium pratense L.) seedlings. 10 (44):8774-8781
7. Atak Ç, Yurttaş B, Alikamanoğlu S, Topçul F, Rzakoulieva A, Danilov V (2000) Effect of magnetic field on Paulownia seeds.
8. Benech Arnold R, Fenner M, Edwards PJNP (1991) Changes in germinability, ABA content and ABA embryonic sensitivity in developing seeds of Sorghum bicolor (L.) Moench. induced by water stress during grain filling. 118 (2):339-347

9. Benlıoğlu B, Özkan U (2020) Germination and Early Growth Performances of Mung Bean (Vigna radiata (L.) Wilczek) Genotypes Under Salinity Stress. 17 (3):318-328. Tekirdağ Ziraat Fakültesi Dergisi, 17(3), 318-328.
10. Burssens S, Himanen K, Van de Cotte B, Beeckman T, Van Montagu M, Inzé D, Verbruggen NJP (2000) Expression of cell cycle regulatory genes and morphological alterations in response to salt stress in Arabidopsis thaliana. 211 (5):632-640
11. Carpýcý E, Celýk N, Bayram GJAJoB (2009) Effects of salt stress on germination of some maize (Zea mays L.) cultivars. 8 (19)
12. Carrow RN, Duncan RR (1998) Salt-affected turfgrass sites: Assessment and management. John Wiley & Sons,
13. Castroluna A, Ruiz O, Quiroga A, Pedranzani HJAeIA (2014) Effects of salinity and drought stress on germination, biomass and growth in three varieties of Medicago sativa L. 18 (1):39-50
14. Chang K-T, Weng C-IJCMS (2008) An investigation into the structure of aqueous NaCl electrolyte solutions under magnetic fields. 43 (4):1048-1055
15. Çulha Ş, ÇAKIRLAR HJAKÜFvMBD (2011) Tuzluluğun bitkiler üzerine etkileri ve tuz tolerans mekanizmaları. 11 (2):11-34
16. Dalia AS, Gendy A, Maria A, Mousa E Response of pepper plants (Capsicum annuum L.) to magnetic technologies. In: Proceedings of the First Nile Delta Conference on Export Crops (Improvement and Protection of the Egyptian Export Crops) Pp, 2009. pp 89-104
17. De Souza A, Garcí D, Sueiro L, Gilart F, Porras E, Licea LJBJotBS, The Society for Physical Regulation in Biology, Medicine TEBA (2006) Pre‐sowing magnetic treatments of tomato seeds increase the growth and yield of plants. 27 (4):247-257
18. Demirkol G, Yilmaz N, Özlem ÖJKSİÜTvDD (2019) Tuz stresinin yem bezelyesi (Pisum sativum ssp. arvense L.) seçilmiş genotipinde çimlenme ve fide gelişimi üzerine etkileri. 22 (3):354-359
19. Ekmekçi E, Mehmet A, Tekin KJATBD (2005) Tuzluluğun bitki gelişimine etkisi. 20 (3):118-125
20. Esechie HJJoA, Science C (1994) Interaction of salinity and temperature on the germination of sorghum. 172 (3):194-199
21. Fatahallah M, Ali F, Gawish R, Hassan DJMJAR (2014) Effect of water regimes and irrigation with magnetic and non-magnetic water on soil salinity and growth of bean plants. 39 (5):1643-1654
22. Foolad M, Lin GJH (1997) Genetic potential for salt tolerance during germination in Lycopersicon species. 32 (2):296-300
23. Gheidary S, Akhzari D, Pessarakli MJJoPN (2017) Effects of salinity, drought, and priming treatments on seed germination and growth parameters of Lathyrus sativus L. 40 (10):1507-1514
24. Grewal HS, Maheshwari BLJB (2011) Magnetic treatment of irrigation water and snow pea and chickpea seeds enhances early growth and nutrient contents of seedlings. 32 (1):58-65
25. Haileselasie THJAJoAS (2012) The effect of salinity (NaCl) on germination of selected grass pea (Lathyrus sativus L.) landraces of Tigray. 4 (2):96-101
26. Hajer A, Malibari AA, Al-Zahrani HS, Almaghrabi OAJAJoB (2006) Responses of three tomato cultivars to sea water salinity 1. Effect of salinity on the seedling growth. 5 (10)
27. Hossain MSJIRJBS (2019) Present scenario of global salt affected soils, its management and importance of salinity research. 1:1-3
28. Houle G, Morel L, Reynolds CE, Siégel JJAJoB (2001) The effect of salinity on different developmental stages of an endemic annual plant, Aster laurentianus (Asteraceae). 88 (1):62-67
29. Hozayn M, EL-Mahdy AA, Abdel-Rahman HJAJoAR (2015) Effect of magnetic field on germination, seedling growth and cytogenetic of onion (Allium cepa L.). 10 (8):849-857
30. Hozayn M, El-Monem A, Elwia T, El-Shatar MJSP-SA, Agronomy (2014) Future of magnetic agriculture in arid and semi arid regions (case study). 57:197-204
31. Ismail WH, Mutwali EM, Salih EA, Elmoula ETJSIJoA, –Volume ES (2020) Effect of Magnetized Water on Seed Germination, Growth and yield of Rocket Plant (Eruca sativa Mill). 7
32. Jones KW, Sanders DJJoST (1987) The influence of soaking pepper seed in water or potassium salt solutions on germination at three temperatures.97-102
33. Kadioğlu BJTZFD (2021) Determination of Germination Biology of Some Sage (Salvia ssp.) Species Under Salinity Stress. Tekirdağ Ziraat Fakültesi Dergisi, 18 (2):359-367
34. Kitajima K, Fenner MJSteoripc (2000) Ecology of seedling regeneration. 2:331-359
35. Kordas LJPJoES (2002) The effect of magnetic field on growth, development and the yield of spring wheat. 11 (5):527-530
36. Kusvuran SJIJoA, Biology (2012) Ion regulation in different organs of melon (Cucumis melo) genotypes under salt stress. 14 (1)
37. Mahmood S, Usman M (2014) Consequences of magnetized water application on maize seed emergence in sand culture.
38. Mansour MJBP (1994) Changes in growth, osmotic potential and cell permeability of wheat cultivars under salt stress. 36 (3):429-434
39. Marcum KBJAWM (2006) Use of saline and non-potable water in the turfgrass industry: Constraints and developments. 80 (1-3):132-146
40. Martinez E, Florez M, Carbonell MJIJoE, Agriculture, Biotechnology (2017) Stimulatory effect of the magnetic treatment on the germination of cereal seeds. 2 (1):375-381
41. Munns R, Tester MJARPB (2008) Mechanisms of salinity tolerance. 59:651-681
42. Mzibra A, Aasfar A, Benhima R, Khouloud M, Boulif R, Douira A, Bamouh A, Kadmiri IMJJoPGR (2021) Biostimulants derived from moroccan seaweeds: seed germination metabolomics and growth promotion of tomato plant. 40 (1):353-370
43. Namba K, Sasao A, Shibusawa SJGEC, 399 A (1994) Effect of magnetic field on germination and plant growth.143-148
44. Nimmi V, Modhu GJIA (2009) Effect of pre-sowing treatment with permanent magnetic field on germination and growth of chilli [Capsicum annum L.]. 23 (2):195-198
45. Oldacay S (2002) Effects of magnetic field on gamma irradiated sunflower (Helianthus annuus L.) varieties. Ph. D. Thesis, Istanbul University, Institute of Science and Engineering,
46. Orchard TJSs, technology (1977) Estimating the parameters of plant seedling emergence.
47. Parida AK, Das ABJE, safety e (2005) Salt tolerance and salinity effects on plants: a review. 60 (3):324-349
48. Passam H, Kakouriotis DJSH (1994) The effects of osmoconditioning on the germination, emergence and early plant growth of cucumber under saline conditions. 57 (3):233-240
49. Pessarakli M, Tucker T, Nakabayashi KJJoPN (1991) Growth response of barley and wheat to salt stress. 14 (4):331-340
50. Pietruszewski S, Kania KJIA (2010) Effect of magnetic field on germination and yield of wheat. 24 (3):297-302
51. Podlesny J, Pietruszewski S, Podlesna AJIA (2004) Efficiency of the magnetic treatment of broad bean seeds cultivated under experimental plot conditions. 18 (1)
52. Qados A, Hozayn MJA-EJoA, Science E (2010) Magnetic water technology, a novel tool to increase growth, yield and chemical constituents of lentil (Lens esculenta) under greenhouse condition. 7 (4):457-462
53. Racuciu M, Creanga D, Galugaru CJRJP (2008) The influence of extremely low frequency magnetic field on tree seedlings. 35:337-342
54. Reina FG, Pascual LAJBJotBS, The Society for Physical Regulation in Biology, Medicine TEBA (2001) Influence of a stationary magnetic field on water relations in lettuce seeds. Part I: Theoretical considerations. 22 (8):589-595
55. Romero-Aranda R, Soria T, Cuartero JJPs (2001) Tomato plant-water uptake and plant-water relationships under saline growth conditions. 160 (2):265-272
56. Sadeghian S, Yavari NJJoA, Science C (2004) Effect of water‐deficit stress on germination and early seedling growth in sugar beet. 190 (2):138-144
57. Samarah NH, Hani B, Makhadmeh IMJH (2021) Effect of Magnetic Treatment of Water or Seeds on Germination and Productivity of Tomato Plants under Salinity Stress. 7 (8):220
58. Scott S, Jones R, Williams WJCs (1984) Review of data analysis methods for seed germination 1. 24 (6):1192-1199
59. Tsegay BA, Gebreslassie BJAJoPS (2014) The effect of salinity (NaCl) on germination and early seedling growth of Lathyrus sativus and Pisum sativum var. abyssinicum. 8 (5):225-231
60. Ul Haq Z, Iqbal M, Jamil Y, Anwar H, Younis A, Arif M, Fareed MZ, Hussain FJIpia (2016) Magnetically treated water irrigation effect on turnip seed germination, seedling growth and enzymatic activities. 3 (2):99-106
61. Van Hoorn JJAWM (1991) Development of soil salinity during germination and early seedling growth and its effect on several crops. 20 (1):17-28
62. Yalçin SJDFBD (2018) The Effect of Magnetic Field on Three Different Varieties of Soybean Seed. 1 (1):1-8
63. Yaycili O, Alikamanoglu SJPC, Tissue, Culture O (2005) The effect of magnetic field on Paulownia tissue cultures. 83 (1):109-114