Interaction of Different Fusarium culmorum Isolates and Pratylenchus thornei on Wheat

Öz

In present study, the interaction of twelve Fusarium culmorum (W. G. Sm.) Sacc. isolates from Isparta, Burdur in Turkey and Pratylenchus thornei (Sher and Allen),1953 were investigated under controlled conditions (25±2°C and 60±5% RH) in Ikizce wheat cultivar. The experiment was established after the first leaves at tillering on wheat and working with four applications. Applications; only P. thornei inoculation (N), only F. culmorum inoculation (FCUL), simultaneous inoculation of P. thornei and F. culmorum (N+FCUL), and R. solani inoculation two weeks after P. thornei application (N+2FCUL) were made. In the inoculation of F. culmorum isolates, 5 ml of 50% diluted culture filtrate concentration was used for each pot, and 1000 larva+adult inoculum density was used for the P. thornei population. Evaluation was carried out 8 weeks after the establishment with disease severity and nematode reproduction rate on wheat. When N+FCUL and N+2FCUL applications were examined in the study, it was observed that P. thornei contributed to the increase of disease severity only in the YLVC16 isolate of F. culmorum. The disease severity scale of YLVC16 isolate was 2.4 in FCUL application, while 3.4 and 3.6 were in N+FCUL and N+2FCUL applications, respectively. The reproduction rate of P. thornei was found to be 2.5 in N application. In the experiment established with YLVC16, T21 and K17 isolates of F. culmorum, the reproduction rate of P. thornei was determined as 3.1, 3.0 and 3.3 in N+FCUL application, while 3.1, 3.2 and 3.4 in N+2FCUL application. Also, in terms of P. thornei reproduction rate, no significant difference was determined between N+FCUL and N+2FCUL applications in the experiment established with YLVC16, T21 and K17 isolates. In the present study, it was determined that only three of the 12 F. culmorum isolates (YLVC16, T21 and K17) contributed positively to the P. thornei reproduction rate, while P. thornei contributed to only one F. culmorum isolate (YLVC16) disease severity.

Anahtar Kelimeler

• Wheat
• Pathogenicity
• Interaction
• Synergistic effect
• Pratylenchus thornei

Proje Numarası

x

Buğdayda Farklı Fusarium culmorum İzolatları ile Pratylenchus thornei Etkileşimi

Öz

Çalışmada Türkiye’nin Isparta ve Burdur illerinden izole edilen on iki Fusarium culmorum (W. G. Sm.) Sacc. izolatı ile Pratylenchus thornei (Sher and Allen),1953 etkileşimi İkizce buğday çeşidinde kontrollü koşullar altında (25±2°C ve %60±5 nem) araştırılmıştır. Buğdaylarda ilk kardeş yapraklar oluştuktan sonra deneme kurulmuş ve 4 uygulamada denemeler yapılmıştır. Uygulamalar; sadece P. thornei uygulaması (N), sadece F. culmorum uygulaması (FCUL), eş zamanlı P. thornei ve F. culmorum uygulaması (N+FCUL) ve P. thornei uygulamasından 2 hafta sonra F. culmorum uygulaması (N+2FCUL) olarak yapılmıştır. Fusarium culmorum izolatlarının inokulasyonunda her saksıya %50 oranında seyreltilmiş kültür filtratı konsantrasyonundan 5 ml, P. thornei popülasyonu için ise 1000 larva+ergin birey inokulum yoğunluğu kullanılmıştır. Değerlendirme işlemi buğdayda hastalık şiddeti ve nematod üreme oranı üzerinden denemenin kurulumundan 8 hafta sonra gerçekleştirilmiştir. Çalışmada N+FCUL ve N+2FCUL uygulamalarına bakıldığında P. thornei’nin sadece F. culmorum’un YLVC16 izolatında hastalık şiddetinin artışına katkısının olduğu görülmüştür. YLVC16 izolatında hastalık şiddeti skala değeri FCUL uygulamasında 2.4 belirlenirken, N+FCUL ve N+2FCUL uygulamalarında sırasıyla 3.4 ve 3.6 tespit edilmiştir. Pratylenchus thornei üreme oranı N uygulamasında 2.5 bulunmuştur. Fusarium culmorum’un YLVC16, T21 ve K17 izolatları ile kurulan denemelerinde P. thornei üreme oranı N+FCUL uygulamasında sırasıyla 3.1, 3.0 ve 3.3 tespit edilirken, N+2FCUL uygulamasında 3.1, 3.2 ve 3.4 saptanmıştır. Ancak P. thornei üreme oranı açısından YLVC16, T21 ve K17 izolatları ile kurulan denemelerde N+FCUL ve N+2FCUL uygulamaları arasında önemli bir farklılık belirlenememiştir. Çalışmada 12 F. culmorum izolatı içerisinden sadece üç tanesinin (YLVC16, T21 ve K17) P. thornei üreme oranına pozitif katkısı belirlenirken, P. thornei’nin ise sadece bir F. culmorum izolatının (YLVC16) hastalık şiddetine katkısının olduğu belirlenmiştir.

Anahtar Kelimeler

• Buğday
• Pratylenchus thornei
• Fusarium culmorum
• Patojenite
• İnteraksiyon
• Sinerjistik etki
• Wheat
• Pathogenicity
• Interaction
• Synergistic effect

Destekleyen Kurum

x

Proje Numarası

x

Teşekkür

Çalışma süresince desteklerini esirgemeyen Zir. Yük. Müh. Nurçin TUNCEL'e teşekkür ederiz.

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