Bazı Origanum Türlerinin Sekonder Metabolitleri ve Uçucu Yağ İçeriklerinin Karşılaştırılması

Öz

Origanum cinsi, biyolojik özelliklerinden dolayı geleneksel tıpta en çok kullanılan şifalı bitkilerden biridir. Bu çalışma, beş Origanum türünün, O. majorana, O. onites, O. Syriacum, O. vulgare subsp. vulgare ve O. vulgare subsp hirtum morfolojik ve fitokimyasal özelliklerini belirlemeyi amaçlamaktadır. Çalışma, İran Urmiye Üniversitesi araştırma alanında yürütülmüştür. Bitkiler bazı kantitatif özellikleri, antioksidan bileşikleri ve uçucu yağ içeriklerini belirlemek için çeşitli yerlerden toplanmıştır. Sonuçlara göre en yüksek bitki boyu (86.4 cm) O. vulgare subsp. hirtum gözlenmiştir. En yüksek taze bitki ağırlığı ve kuru ağırlığı diğer türler ile karşılaştırıldığında O. onites (826 ve 250 g) ve O. vulgare subsp. hirtum (727.64 ve 230 g) türlerinde görülmüştür. Fakat en yüksek uçucu yağ, bitki başına uçucu yağ verimi ve hektar başına uçucu yağ verimi sırasıyla %5.26, 1.71 g ve 114 kg/ha ile O. vulgare subsp hirtum türünde gözlenmiştir. Kantitatif analiz sonuçlarına göre; en yüksek toplam fenol içeriği (%51.12), flavonoid (%6.93), 2, 2-difenil-1-pikrilhidrazil (DPPH), (%54.29), süperoksit (%50.04) içeriği ve radikal yakalama aktiviteleri O. vulgare subsp. hirtum’da ortaya konmuştur, ancak diğer türlerle karşılaştırıldığında, O. onites daha yüksek (%21.60) nitrik oksit radikali yakalama aktiviteleri göstermiştir. Uçucu yağ analizleri, Origanum cinsinin incelenen türlerinde, timol (%6.90-59.89), karvakrol (%0.83-48.91), γ-terpinen (%6.18-55.20), p-simen (%94 0.50-0.20) ve a-terpinenin (%2.71-) temel bileşenler olduğunu göstermiştir. Küme analizi, O. onites ve O. vulgare subsp hirtum arasında iki ana kategori ve yüksek benzerlik göstermektedir. Bu araştırmanın bulgularına göre fitokimyasal özellikler açısından O. vulgare subsp hirtum’un en iyi tür olduğu belirlenmiştir.

Anahtar Kelimeler

• Mercanköşk
• Fitokimyasallar
• Sekonder Metabolitler
• Timol

Etik Beyan

Bu çalışma için etik kuruldan izin alınmasına gerek yoktur.

Teşekkür

The authors are grateful to the Office of Vice Chancellor, Urmia University and Siirt University for supporting this study.

Comparison of Secondary Metabolites and Essential Oil Content of Some Origanum Species

Öz

Origanum genus is one of the most widely used herbs in folk medicine for its biological properties. This study was performed to determine the morphological and phytochemical properties of five species of Origanum including O. majorana, O. onites, O. syriacum, O. vulgare subsp. vulgare and O. vulgare subsp. hirtum as important ethnomedicinal plants. The study was performed at a Research Farm based at Urmia University, Iran. The plants were collected from various places for determining some quantitative properties, antioxidant compounds, and essential contents. The results showed that the highest plant height (86.4 cm) was observed in O. vulgare subsp. hirtum. The highest fresh weight and dry weight were observed in O. onites (826 and 250 g) and O. vulgare subsp. hirtum (727.64 and 230 g) species in comparison to others, respectively. However, the highest essential oil, essential oil yield per plant, and essential oil yield per ha were 5.26%, 1.71 g and 114 kg ha-1, respectively, which was observed in O. vulgare subsp. hirtum species. The quantitative analysis revealed higher content of total phenol (51.12%), flavonoid (6.93%), 2, 2-diphenyl-1-picrylhydrazyl (DPPH), (54.29%), superoxide (50.04%) and radical scavenging activities in O. vulgare subsp. hirtum species, but the O. onites species showed higher (21.60%) nitric oxide radical scavenging activities compared to other species. The essential oil analysis revealed that the thymol (6.90-59.89%), carvacrol (0.83-48.91%), γ-terpinene (6.55-18.20%), p-cymene (0.50-20.94%) and α-terpinene (2.71-4.28%) were the most prominent compounds in the studied species of the genus Origanum. Cluster analysis showed two main categories and high similarity between O. onites and O. vulgare subsp. hirtum. The findings of the current research indicate that O. vulgare subsp. hirtum was the best species in terms of phytochemical properties.

Anahtar Kelimeler

• Marjoram
• Phytochemicals
• Secondary metabolites
• Thymol

Etik Beyan

There is no need to obtain permission from the ethics committee for this study.

Teşekkür

The authors are grateful to the Office of Vice Chancellor, Urmia University and Siirt University for supporting this study.

Kaynakça

1. Adams, R. P. (2007). Identification of Essential oil Components by gas Chromatography/Quadrupole Mass Spectrometry, 4th ed. Allured Publ. Corp, Carol Stream.
2. Aktepe, B. P., Mertoğlu, K., Evrenosoğlu, Y. and Aysan, Y. (2019). Determination of antibacterial effect of different plant essential oils against Erwinia amylovora. Journal of Tekirdag Agricultural Faculty, 16: 34-41. https://doi.org/10.33462/jotaf.516848
3. Amiri, N., Yadegari, M. and Hamedi, B. (2018). Essential oil composition of Cirsium arvense L. produced in different climate and soil properties. Records of Natural Products, 12: 251.-262. https://doi.org/10.25135/rnp.27.17.06.043
4. Bhardwaj, K. and Dubey, W. (2019). Quantitative analysis of primary and secondary metabolites of ethanol seed extract of Origanum majorana (Marjoram). Journal of Pharmacogn Phytochem, 8:1251-1255.
5. Bonea, D. and Urechean, V. (2018). Effect of sweet marjoram (Origanum majorana L.) cogermination and aqueous extracts on maize (Zea mays L.). Agriculture for Life, Life for Agriculture, 1: 11-16. https://doi.org/10.2478/alife-2018-0002
6. Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28:25-30.
7. Duletić-Laušević, S., Aradski, A.A., Kolarević, S., Vuković-Gačić, B., Oalđe, M., Živković, J., Šavikin, K.and Marin, P.D. (2018). Antineurodegenerative, antioxidant and antibacterial activities and phenolic components of Origanum majorana L. (Lamiaceae) extracts. Journal of Applied Botany and Food Quality, 91: 126-134. https://doi.org/10.5073/JABFQ.2018.091.018
8. Elshamy, A.I., Mohamed, T.A., Marzouk, M.M., Hussien, T.A., Umeyama, A., Hegazy, M.E.F. and Efferth, T. (2018). Phytochemical constituents and chemosystematic significance of Pulicaria jaubertii E. Gamal-Eldin (Asteraceae). Phytochemistry Letters, 24: 105-109. https://doi.org/10.1016/j.phytol.2018.01.021

9. Faridvand, S., Rezaei-Chiyaneh, E., Battaglia, M., Gitari, H., Raza, M.A. and Siddique, K.H.M. (2021). Application of bio and chemical fertilizers improves yield, and essential oil quantity and quality of Moldavian balm (Dracocephalum moldavica L.) intercropped with mung bean (Vigna radiata L.). Food and Energy Security, p. e319. https://doi.org/10.1002/fes3.319
10. Garrett D. (1964). The quantitative Analysis of Drugs. Chapman and Hall, Japan, 3: 456-458. Gayoso, L., Claerbout, A.S., Calvo, M.I., Cavero, R.Y., Astiasarán, I. and Ansorena, D. (2016). Bioaccessibility of rutin, caffeic acid and rosmarinic acid: Influence of the in vitro gastrointestinal digestion models. Journal of Functional Foods, 26: 428-438. https://doi.org/10.1016/j.jff.2016.08.003
11. Gayoso, L., Roxo, M., Cavero, R.Y., Calvo, M.I., Ansorena, D., Astiasarán, I. and Wink, M. (2018). Bioaccessibility and biological activity of Melissa officinalis, Lavandula latifolia and Origanum vulgare extracts: Influence of an in vitro gastrointestinal digestion. Journal of Functional Foods, 44: 146-154. https://doi.org/10.1016/j.jff.2018.03.003
12. Hassan, M.J., Raza, M.A., Rehman, S.U., Ansar, M., Gitari, H., Khan, I., Wajid, M., Ahmed, M., Shah, G.A., Peng, Y. and Li, Z. (2020). Effect of cadmium toxicity on growth, oxidative damage, antioxidant defense system and cadmium accumulation in two sorghum cultivars. Plants, 9(11): 1575. https://doi.org/10.3390/plants9111575
13. Heydarzadeh, S., Gitari, H., Rahimi, A., Khiavi, H.K., Maitra, S. And Hosseinpour, A. (2021a). Yield and quality traits of field-grown sugar beet (Beta vulgaris L.) in response to foliar application of micronutrients and different levels of manure. International Journal of Bioresource Science, 8(2): 103–112.
14. Heydarzadeh, S., Hosseinpour, A., Gitari, H., Maitra, S. and Rahimi, A. (2021b). Improving quantitative and qualitative properties of cotton through the use of organic and chemical fertilizers under water deficit stress conditions. International Journal of Bioresource Science. 8(2): 69–80.
15. Heydarzadeh, S., Jalilian, J., Pirzad, A., Jamei, R. and Petrussa, E. (2022). Fodder value and physiological aspects of rainfed smooth vetch affected by biofertilizers and supplementary irrigation in an agri-silviculture system. Agroforestry Systems, 96: 1-12.
16. Jing, T.Y. and Zhao, X.Y. (1995). The improved pyrogallol method by using terminating agent for superoxide dismutase measurement. Progress in Biochemistry and Biophysics, 22: 84-86.
17. Kaplan, M., Mücahit Yilmaz, M., Uslu, R., Köprü, S., Gözelle, H. and Muhderem, G. (2019). An optimization study for the production of Origanum onites tincture by response surface methodology: effect of liquid/solid ratio, ethanol concentration and storage period. Journal of Tekirdag Agricultural Faculty, 16: 11-22.
18. Koleva, V., Dragoeva, A., Stoyanova, Z., Yordanova, Z., Ali, S., Uzunov, N.M., Melendez-Alafort, L., Rosato, A. and Enchev, D.D. (2018). In vitro cytotoxicity of allelopathic plants L. ssp. L. and subsp. Acta Scientiarum Naturalium, 5: 64-69.
19. Kosakowska, O., Węglarz, Z. and Bączek, K. (2019). Yield and quality of ‘Greek oregano’ (Origanum vulgare L. subsp. hirtum) herb from organic production system in temperate climate. Industrial Crops and Products, 141(10):111782 https://doi.org/10.1016/j.indcrop.2019.111782
20. Marrelli, M., Statti, G.A. and Conforti, F. (2018). Origanum spp.: an update of their chemical and biological profiles. Phytochemistry Reviews, 17:873-888.
21. Méabed, E.M., El-Sayed, N.M., Abou-Sreea, A.I. and Roby, M.H. (2018). Chemical analysis of aqueous extracts of Origanum majorana and Foeniculum vulgare and their efficacy on Blastocystis spp. cysts. Phytomedicine, 43: 158-163.
22. Mirriam, A., Mugwe, J., Raza, M.F., Seleiman, M.A., Maitra, S. and Gitari, H.I. (2022). Aggrandizing soybean yield, phosphorus use efficiency and economic returns under phosphatic fertilizer application and inoculation with Bradyrhizobium. Journal of Soil Science and Plant Nutrition, 22: 5086- 5098. https://doi.org/10.1007/s42729-022-00985-8
23. Mirzapour, M., Valizadeh, N., Heydarzadeh, S., Rahimi, A. and Gitari, H. (2022). Factors Affecting the Production of Secondary Metabolites in Medicinal Plants. Pg 3-32. In: Ozyazıcı G (Ed). New Development on Medicinal and Aromatic Plants-II, Iksad publishing house, Turkey.
24. Moghrovyan, A., Sahakyan, N., Babayan, A., Chichoyan, N., Petrosyan, M. and Trchounian, A. (2019). Essential oil and ethanol extract of oregano (Origanum vulgare L.) from armenian flora as a natural source of terpenes, flavonoids and other phytochemicals with antiradical, antioxidant, metal chelating, tyrosinase inhibitory and antibacterial activity. Current Pharmaceutical Design, 25: 1809-1816.
25. Moreno, Y. and Arteaga-Miñano, H. (2018). Natural conservation of guinea pig (Cavia porcellus) meat vacuum packed: Oregano essential oil effect on the physicochemical, microbiological and sensory characteristics. Scientia Agropecuaria, 9: 467-476.
26. Morshedloo, M. R., Salami, S.A., Nazeri, V., Maggi, F. and Craker, L. (2018). Essential oil profile of oregano (Origanum vulgare L.) populations grown under similar soil and climate conditions. Industrial Crops and Products, 119: 183-190.
27. Mwadalu, R., Mochoge, B., Mwangi M., Maitra S. and Gitari, H. (2022). Response of Gadam sorghum (Sorghum bicolor) to farmyard manure and inorganic fertilizer application. International Journal of Agriculture, Environment, and Biotechnology, 15: 51–60. https://doi.org/10.30954/0974-1712.01.2022.6
28. Ngugi, M. M., Gitari, H. I., Muui C. and Gweyi-Onyango, J. P. (2021). Cadmium mobility, uptake and accumulation in spinach, kale and amaranths vegetables as influenced by Silicon fertilization. Bioremediation Journal, 26(2): 113–127. https://doi.org/10.1080/10889868.2021.1924111
29. Oke-Altuntas, F., Demirci, M.A., Demirtas, I., Yaglioglu, A. S. and Behcet, L. (2018). Phytochemical screening, antiproliferative and antioxidant properties of various extracts from endemic origanum acutidens. Combinatorial Chemistry & High Throughput, 21: 281-291.
30. Pandey, A., Belwal, T., Tamta, S., Bhatt, I. and Rawal, R. (2019). Phenolic compounds, antioxidant capacity and antimutagenic activity in different growth stages of in vitro raised plants of Origanum vulgare L. Molecular Biology Reports, 46:2231-2241. https://doi.org/10.1007/s11033-019-04678-x
31. Pezzani, R., Vitalini, S. and Iriti, M. (2017). Bioactivities of Origanum vulgare L.: an update. Phytochemistry Reviews, 16: 1253-1268.
32. Rahimi, A., Mohammadi, M.M., Moghadam, S.S., Heydarzadeh, S. and Gitari, F. (2022). H. Effects of stress modifier biostimulants on vegetative growth, nutrients and antioxidants contents of garden thyme (Thymus vulgaris L.) under water stress. Journal of Plant Growth Regulation, 41: 2059–2072. https://doi.org/10.1007/s00344-022-10604-6
33. Ramakrishna, R., Sarkar, D. and Shetty, K. (2019). Metabolic stimulation of phenolic biosynthesis and antioxidant enzyme response in dark germinated barley (Hordeum vulgare L.) sprouts using bioprocessed elicitors. Food Science and Biotechnology, 28: 1093-1106.
34. Raudone, L., Motiekaityte, V., Vainoriene, R., Zymone, K., Marksa, M. and Janulis, V. (2018). Phytochemical profiles of alpine plant Horminum Pyrenaicum L. during phenological growth stages. Chemistry & Biodiversity, 15: e1800190. https://doi.org/10.1002/cbdv.201800190
35. Salvo, A., La Torre, G.L., Rotondo, A., Cicero, N., Gargano, R., Mangano, V., Casale, K.E. and Dugo, G. (2018). Multiple analytical approaches for the organic and inorganic characterization of Origanum vulgare L. samples. Natural Product Research, 33: 2815-2822. https://doi.org/10.1080/14786419.2018.1503269
36. Sefeer, K. and Elumalai, K. (2018). Larvicidal activity of plant oil, Origanum majorana L. against the selected mosquito larvae and GC-MS analysis of its phytochemical compounds. Annals of the Entomological Society of America, 36: 01-05.
37. Shafiee-Hajiabad, M., Hardt, M. and Honermeier, B. (2014). Comparative investigation about the trichome morphology of Common oregano (Origanum vulgare L. subsp. vulgare) and Greek oregano (Origanum vulgare L. subsp. hirtum). Journal of Applied Research on Medicinal and Aromatic Plants, 1(2): 50-58. https://doi.org/10.1016/j.jarmap.2014.04.001
38. Singleton, V.L., Orthofer, R. and Lamuela-Raventós, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299: 152-178.
39. Spyridopoulou, K., Fitsiou, E., Bouloukosta, E., Tiptiri-Kourpeti, A., Vamvakias, M., Oreopoulou, A., Papavassilopoulou, E., Pappa, A. and Chlichlia, K. (2019). Extraction, chemical composition, and anticancer potential of Origanum onites L. essential oil. Molecules, 24(14):2612. https://doi.org/10.3390/molecules24142612
40. Svidenko, L., Grygorieva, O., Vergun, O., Hudz, N., Horˇcinová, E., Sedláˇcková, V., Šimkowá, J. and Brindza, J. (2018). Characteristic of leaf peltate glandular trichomes and their variability of some Lamiaceae family species. Agrobiodiversity, 10: 124–131. https://doi.org/10.15414/agrobiodiversity.2018.2585-8246.124-132
41. Tripathy, B., Satyanarayana, S., Khan, K. A. and Raja, K. (2017). An updated review on traditional uses, taxonomy, phytochemistry, pharmacology and toxicology of Origanum majorana. International Journal of Pharma Research and Health Sciences, 5:17-23.
42. Węglarz, Z., Kosakowska, O., Przybył, J. L., Pióro-Jabrucka, E. and Bączek, K. (2020). The quality of Greek oregano (O. vulgare L. subsp. hirtum (Link) Ietswaart) and common oregano (O. vulgare L. subsp. vulgare) cultivated in the temperate climate of central Europe. Foods, 9(11); 1671. https://doi.org/10.3390/foods9111671