Assessment of Genetic Diversity Among Fusarium Species Isolated from Wheat Using SCoT Markers

Yıl 2026, Cilt: 23 Sayı: 1, 21 - 31, 07.01.2026

Göksel Özer , Müberra Yeken , Sibel Derviş , Gülsüm Palacıoğlu

https://doi.org/10.33462/jotaf.1469186

Öz

Fusarium species pose significant threats to wheat cultivation by inducing root rot, crown rot, and head blight. Understanding the genetic variations within these species is pivotal for effective disease management strategies. This investigation aimed to evaluate the genetic variation among isolates of Fusarium culmorum, F. graminearum, and F. pseudograminearum following their identification via cultural and species-specific PCR assays. The application of species-specific PCR facilitated the precise identification of these species by generating bands of distinct sizes (F. culmorum: 570 bp, F. pseudograminearum: 520 bp; F. graminearum: 420 bp), aligning with morphological classifications. A total of 36 Start Codon Targeted (SCoT) markers were employed to characterize the genetic variability among Fusarium isolates. Among the selected SCoT primers, 181 bands were amplified, with 131 exhibiting polymorphism (72.37%). The percentages of polymorphic bands in primers varied between 61.54 and 89.29. The highest polymorphism locus was observed in Fusarium culmorum with 12.71%, followed by F. pseudograminearum and F. graminearum. The average polymorphism information content and resolving power for the SCoT markers were calculated as 0.26 and 10.04, respectively. Utilizing the unweighted pair group method with arithmetic mean (UPGMA) clustering analysis employing Dice's method, the SCoT data delineated the Fusarium isolates into three distinct clusters corresponding to species. The study which focused on specifically evaluating the genetic diversity of Fusarium culmorum, F. graminearum and F. pseudograminearum, recognized as primary threats to global wheat cultivation, with SCoT markers, substantial genetic variations were revealed among Fusarium isolates at both inter- and intra-species levels. This investigation represents the initial endeavor to elucidate the genetic diversity of Fusarium isolates obtained from wheat utilizing SCoT markers.

Anahtar Kelimeler

Fusarium spp. , Genetic diversity , Root rot , SCoT markers , Wheat

Etik Beyan

There is no need to obtain permission from the ethics committee for this study.

Destekleyen Kurum

The work received partial financing from TUBITAK (Türkiye’s Scientific and Technological Research Council).

Proje Numarası

1919B012004078

Buğdaydan İzole Edilen Fusarium Türlerinde SCoT Markörleri Kullanılarak Genetik Çeşitliliğin Değerlendirilmesi

Öz

Fusarium türleri kök çürüklüğü, kökboğazı çürüklüğü ve başak yanıklığına neden olarak buğday yetiştiriciliğinde ciddi zararlara neden olmaktadır. Bu türler içerisindeki genetik varyasyonun anlaşılması, etkili hastalık yönetimi stratejileri için oldukça önemli bir yere sahiptir. Bu çalışmanın amacı, kültürel yöntemler ve tür özgü PCR testleri aracılığıyla Fusarium culmorum, F. graminearum ve F. pseudograminearum izolatlarının tanımlanmasının ardından, bu türler arasındaki genetik çeşitliliği değerlendirmektir. Türe özgü PCR uygulaması, morfolojik sınıflandırmalarla uyumlu olarak çeşitli boyutlarda bantlar (F. culmorum: 570 bp, F. pseudograminearum: 520 bp; F. graminearum: 420 bp) oluşturmuştur. Fusarium izolatları içindeki genetik değişkenliği karakterize etmek için toplam 36 adet başlangıç kodonunu hedefleyen (SCoT) markör kullanılmıştır. Seçilen SCoT primerlerinden 181 bant amplifiye edilmiş ve bunların 131'i (%72.37) polimorfizm göstermiştir. Primerler arasındaki polimorfik band yüzdeleri 61.54 ve 89.29 arasında değişim göstermiştir. En yüksek polimorfizm lokusu %12.71 ile Fusarium culmorum izolatlarında görülmüş, bunu F. pseudograminearum ve F. graminearum izolatları takip etmiştir. SCoT markörleri için ortalama polimorfizm bilgi içeriği ve çözünürlük gücü sırasıyla 0.26 ve 10.04 olarak hesaplanmıştır. Dice'in yöntemine göre aritmetik ortalamalı ağırlıksız çift grup yöntemi (UPGMA) kümeleme analiz kullanılarak, SCoT verileri Fusarium izolatlarını türlerle uyumlu bir şekilde üç ayrı kümeye ayırmıştır. Küresel buğday yetiştiriciliğine yönelik birincil tehditler olarak kabul edilen F. culmorum, F. graminearum ve F. pseudograminearum'un genetik çeşitliliğinin SCoT markörleri ile değerlendirilmesine özel olarak odaklanan bu çalışmada, Fusarium izolatları arasında hem türler arası hem de tür içi seviyelerde önemli genetik varyasyonlar ortaya konulmuştur. Bu araştırma, başlangıç kodonunu hedefleyen belirteçlerin kullanılarak buğdaydan elde edilen Fusarium izolatlarının genetik çeşitliliğini belirlemek amacıyla SCoT markörlerinin kullanıldığı ilk çalışma niteliğindedir.

Anahtar Kelimeler

Buğday , Fusarium spp. , Genetik çeşitlilik , Kök çürüklüğü , SCoT markörleri

Etik Beyan

There is no need to obtain permission from the ethics committee for this study.

Destekleyen Kurum

The work received partial financing from TUBITAK (Türkiye’s Scientific and Technological Research Council).

Proje Numarası

1919B012004078

Kaynakça

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