Determination of PVY Distribution in Section of Seed Potato Tubers Tohumluk Patates Yumru Kesitlerinde Patates Y Virüsü Dağılımının Belirlenmesi

Öz

Potato virus Y (PVY) (species Potyvirus yituberosi, genus Potyvirus, family Potyviridae) is a plant pathogenic virus that seriously affects potato production. PVY is one of the mandatory plant pathogenic viruses that are tested in the seed potato tuber certification scheme. The primary method of managing PVY is to produce or provide virus-free seed tubers. However, reliably detecting the virus in tubers remains rather challenging and is an important subject of field research. An investigation was conducted to explore the localization of PVY within infected tubers of the highly susceptible cultivar, Desire, to determine its distribution across different tuber sections. Samples were taken from the apical bud, stem bud, lateral eyes, and pith sections of the dormant tubers, and tested using a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and Immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR). The DAS-ELISA detected PVY in 50% of samples (5/10), with positive results only in apical buds. However, IC-RT-PCR achieved higher sensitivity for detecting PVY in all the apical buds (10/10; 100%). Specifically, in stem buds tested positive in 80% of cases (8/10), while lateral buds yielded detection rates of 80% (8/10) and 50% (5/10). IC-RT-PCR consistently identified PVY in all examined tubers, demonstrating its effectiveness for virus detection. The results indicate that sampling only the apical bud is sufficient for PVY detection using either DAS-ELISA or IC-RT-PCR. The pith and deep tissue below the bark of the potato tuber were unsuitable for PVY detection. For personnel with less experience, who may struggle to distinguish between apical and stem buds, these two sections can be combined, provided the apical bud is included. In future investigations, it is crucial to acknowledge that the virus titer may vary with potato variety and PVY strain. This study's applicability extends to diverse potato varieties and virus strains for comprehensive exploration.

Anahtar Kelimeler

• Potato
• PVY
• Distribution
• Tuber sections
• DAS-ELISA
• IC-RT-PCR

Tohumluk Patates Yumru Kesitlerinde Patates Y Virüsü Dağılımının Belirlenmesi

Öz

Patates Y virüsü (PVY) fitopatojen bir virüstür ve patates üretimini etkileyen en önemli patojenlerden biridir. PVY, tohumluk patates yumrusu sertifikasyon sisteminde test edilmesi zorunlu bitki patojenik virüslerden biridir. PVY ile mücadelede birincil yöntem, sağlıklı tohumluk yumruları üretmek veya temin etmektir. Ancak, yumrularda virüsü güvenilir bir şekilde tespit etmek oldukça zorlu ve önemli bir araştırma konusudur. Bu çalışma, PVY ile enfekteli ve etmene son derece duyarlı Desire çeşidi patates yumrularının farklı kesitlerinde virüsün dağılımını araştırmak için yürütülmüştür. Araştırma için örnekler dormant yumruların apikal tomurcuğundan, gövde tomurcuğundan, lateral gözlerinden ve öz kısımlarından alınarak, DAS-ELISA ve IC-RT-PCR kullanılarak teste tabi tutulmuştur. DAS-ELISA örneklerinin %50’sinde (5/10) PVY tespit etmiştir ve sadece apikal tomurcuklarda pozitif sonuçlar vermiştir. Ancak, IC-RT-PCR, tüm apikal tomurcuklarda PVY’yi tespit etmede daha yüksek hassasiyete ulaşmıştır (%100, 10/10). Özellikle, IC-RT-PCR gövde tomurcuklarında %80 (8/10), lateral tomurcuklarda ise %80 (8/10) ve %50 (5/10) oranında tespit edebilme başarısı göstermiştir. IC-RT-PCR, incelenen tüm yumrularda PVY'yi tutarlı bir şekilde tespit ederek virüs tanımlamada başarılı bir şekilde kullanılabilecek yöntem olarak belirlenmiştir. Sonuçlar, sadece apikal tomurcuğun örneklemesinin PVY tespit için yeterli olduğunu göstermektedir. Patates yumrusunun kabuğunun altındaki öz ve derin doku, yumruda PVY tespiti için kullanışlı değildir. Hem DAS-ELISA hem de IC-RT-PCR yöntemleri kullanılarak PVY tespiti için apikal tomurcuk kullanılmalıdır. Apikal ve kök tomurcuklarını ayırt edemeyen daha az deneyimli personel için, apikal tomurcuk dahil edilmesi koşuluyla bu iki bölüm birleştirilebilir. Gelecekteki araştırmalarda, virüs titresinin patates çeşidine ve PVY'nin spesifik strainine göre değişebileceğini dikkate almak çok önemlidir. Bu çalışma, farklı patates çeşitlerine ve virüs strainlerinde de uygulanabilir.

Anahtar Kelimeler

• Patates
• PVY
• Dağılım
• Yumru alanları
• DAS-ELISA
• IC-RT-PCR

Kaynakça

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