Salicylic Acid Seed Priming Enhances Lentil Germination and Seedling Vigor under Salinity Stress

Öz

Salicylic acid (SA), a plant-derived phenolic compound, acts as a signaling molecule that regulates controlling diverse plant responses and plays a role in plant defense under stress conditions. This study aimed to assess the potential of SA seed priming in mitigating the negative effects of salt stress on lentil germination and early seedling development. The germination and vigor of seeds primed with five different doses of SA (0, 0.25, 0.5, 0.75, and 1 mM) were tested at five salinity levels (0, 50, 100, 150 and 200 mM) in peat-perlite. The research was conducted using a completely randomized design with factorial arrangement under greenhouse conditions. Results indicated that low concentrations of SA (0.25 mM) had positive effects on days to seedling emergence, emergence energy (EE), emergence percentage (EP), emergence rate index (ERI), mean emergence time (MET), peak value (PV) and seedling vigor index suggesting that SA can enhance germination and seedling emergence under high salinity. However, as SA concentration increased, a decrease in emergence rate was observed, indicating that higher SA levels may have inhibitory effects on seed performance after sowing. The results also show that SA pre-treatment can significantly improve shoot length, root length, fresh shoot weight, and fresh root weight of lentil seedlings exposed to NaCl stress. Adverse effects of increasing NaCl concentrations were noted for all measured traits, with shoot length, root length, fresh shoot weight, and fresh root weight decreasing, and, no growth observed at the highest NaCl concentration (200 mM). In conclusion, SA can be effectively used in seed priming to mitigate salinity stress during early lentil growth, but the effectiveness of this treatment depends on the salinity level.

Anahtar Kelimeler

• Abiotic stress
• Emergence
• Growth
• Hormone
• Lens culinaris L.

Tuz Stresi Altında Salisilik Asit Tohum Ön Uygulaması ile Mercimekte Çimlenme ve Fide Canlılığının Arttırılması

Öz

Bitkisel kaynaklı bir fenolik bileşik olan salisilik asit (SA), çeşitli bitki tepkilerini kontrol eden bir sinyal molekülü olup stres şartlarında bitki savunmasında rol almaktadır. Bu çalışma, tuz stresinin mercimek çimlenmesi ve erken fide gelişimi üzerindeki olumsuz etkilerini azaltmada salisilik asit ile gerçekleştirilen tohum ön işlem uygulamasının etkisini değerlendirmeyi amaçlamaktadır. Beş farklı salisilik asit dozu (0, 0.25, 0.5, 0.75 ve 1 mM) ile ön işlem uygulanan tohumların çimlenme ve canlılığı 5 farklı tuz seviyesinde (0, 50, 100, 150 ve 200 mM) torf-perlit ortamında test edilmiştir. Deneme faktöriyel düzende tesadüf parselleri deneme deseninde sera koşullarında yürütülmüştür. Sonuçlar, düşük konsantrasyonlardaki salisilik asidin (0.25 mM), fide çıkış zamanı, çıkış enerjisi (EE), çıkış yüzdesi (EP), çıkış oran indeksi (ERI), ortalama çıkış süresi (MET), pik değer (PV) ve fide canlılık indeksi gibi parametreler üzerinde olumlu etkilerini ve SA’in yüksek tuzlu koşullar altında çimlenmeyi ve fide çıkışını artırabileceğini göstermiştir. Ancak, SA konsantrasyonu arttıkça, çıkış oranında azalma tespit edilmiş olup, yüksek SA seviyesinin ekimden sonra tohum performansı üzerinde engelleyici etkilere sahip olabileceğini düşündürmüştür. Sonuçlar, SA ile ön işlem uygulamasının NaCl stresine maruz kalan mercimek fidelerinin sürgün uzunluğunu, kök uzunluğunu, taze sürgün ağırlığını ve taze kök ağırlığını önemli ölçüde iyileştirebileceğini göstermektedir. Artan NaCl konsantrasyonlarında sürgün uzunluğu, kök uzunluğu, taze sürgün ağırlığı ve taze kök ağırlığı dahil olmak üzere gözlenen tüm özellikler için olumsuz etkiler tespit edilmiş ve en yüksek tuz konsantrasyonu 200 mM seviyesinde büyüme gözlemlenmemiştir. Sonuç olarak, salisilik asit ile ön işlem uygulamasının tuzluluk düzeyine bağlı olarak etkinliğinin değişiklik göstermesine rağmen mercimeğin erken büyüme aşamalarında tuzluluk stresini azaltmada etkili bir araç olarak kullanılabileceğini göstermektedir.

Anahtar Kelimeler

• Abiyotik stres
• Çıkış
• Büyüme
• Hormon
• Lens culinaris L.

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