Impact of Salinity and Drought Stress on Germination of Asclepias curassavica L.

Yıl 2026, Cilt: 23 Sayı: 2, 545 - 553, 16.03.2026

Işın Kocabaş Oğuz

https://doi.org/10.33462/jotaf.1646291

https://izlik.org/JA97UT63LG

Öz

In this study, the germination performance of Asclepias curassavica L. seeds under salinity and drought stress, as well as their effects on radicle and plumule, were investigated. For the salinity stress experiment, solutions were prepared using 6 different NaCl at 0, 50, 100, 150, 200 and 250 mM. To simulate drought stress, six distinct levels of water stress were created using polyethylene glycol-6000 (PEG 6000) solutions with varying water retention capacities, encompassing 0, -2, -4, -6, -8, and -9.8 bar. Germination trials were carried out with 20 seeds in each petri dish according to the random plots experimental design with four replications. In order to examine the germination performances of the seeds, the petri dishes in which the solutions to create the stress conditions were applied and the seeds were placed were tested for 15 days in a controlled germination cabinet with a constant ambient temperature of 20°C, 70% humidity, 14 hours of light, and 10 hours of dark conditions. According to the results obtained, in salinity stress applications, the germination rate of Asclepias curassavica L. seeds ranged from 100.00% to 5.00%; the radicle length varied between 34.34 mm and 2.65 mm; the plumule length ranged from 35.11 mm to 8.11 mm; the fresh weight of the radicle fluctuated between 9.38 mg and 0.70 mg; and the fresh weight of the plumula varied from 20.25 mg to 5.88 mg. In drought stress treatments, the germination rate of Asclepias curassavica L. seeds ranged from 100.00% to 65.00%; radicle length ranged from 33.86 mm to 5.89 mm; plumule length ranged from 23.86 mm to 10.04 mm; radicle fresh weight ranged from 8.95 mg to 2.53 mg; and plumule fresh weight ranged from 15.55 mg to 5.58 mg. The study indicates that low to moderate salt levels did not hinder the germination of Asclepias curassavica seeds, but salt stress could lead to a reduction in radicle and plumule growth, resulting in disproportionate development of the plants. Additionally, it was concluded that the germination of Asclepias curassavica seeds was preserved under drought stress up to a certain level, with root and shoot growth increasing compared to plants that were not exposed to stress. These findings highlight the species' ability to adapt under stress conditions.

Anahtar Kelimeler

Germination , Asclepias curassavica , Salinity , Drought stress , Radicle , Plumule

Etik Beyan

There is no need to obtain permission from the ethics committee for this study.

Tuzluluk ve Kuraklık Stresinin Asclepias curassavica L.'nin Çimlenmesine Etkisi

https://izlik.org/JA97UT63LG

Öz

Bu çalışmada Asclepias curassavica L. türünün tuzluluk ve kuraklık stresine karşı tohumların çimlenme performansları, radikula ve plumula üzerindeki etkileri incelenmiştir. Tuzluluk stresi deneyi için çözeltiler altı farklı NaCl konsantrasyonu ile formüle edilmiştir: 0, 50, 100, 150, 200 ve 250 mM. Kuraklık stresine benzer ortam sağlamak için su tutma kapasitesi yüksek polietilen glikol-6000 (PEG 6000) çözeltisi kullanılmış ve 0, -2, -4, -6, -8 ve -9,8 bar dahil olmak üzere altı farklı su stresi derecesi oluşturulmuştur. Çimlendirme denemeleri her petri kutusunda 20 tohum olacak şekilde tesadüf parselleri deneme desenine göre dört tekrarlamalı olarak yürütülmüştür. Tohumların çimlenme performanslarını incelemek amacıyla, stres koşullarını oluşturacak olan çözeltilerin uygulandığı ve tohumların bulunduğu petri kapları; ortam sıcaklığı 20oC’de sabit olan, %70 nem 14 saat aydınlık 10 saat karanlık koşullarda, kontrollü bir çimlendirme kabini içerisinde 15 gün süresince teste tabi tutulmuştur. Elde edilen sonuçlara göre, tuzluluk stresi uygulamalarında Asclepias curassavica L. tohumlarının çimlenme oranı %100.00 ile %5.00 arasında; radikula uzunluğu 34.34 mm ile 2.65 mm arasında; plumula uzunluğu 35.11 mm ile 8.11 mm arasında; radikula yaş ağırlığı 9.38 mg ile 0.70 mg arasında ve plumula yaş ağırlığı 20.25 mg ile 5,88 mg arasında değişmiştir. Kuraklık stresi uygulamalarında ise, Asclepias curassavica L. tohumlarının çimlenme oranı %100.00 ile %65.00 arasında; radikula uzunluğu 33.86 mm ile 5.89 mm arasında; plumula uzunluğu 23.86 mm ile 10,04 mm arasında; radikula yaş ağırlığı 8.95 mg ile 2.53 mg arasında ve plumula yaş ağırlığı 15.55 mg ile 5.58 mg arasında değişiklik göstermiştir. Çalışma, düşük ile orta tuz seviyelerinin Asclepias curassavica tohumlarının çimlenmesini engellemediğini, ancak tuz stresinin radikula ve plumula büyümesindeki azalmaya yol açarak bitkilerin orantısız gelişimine neden olabileceğini göstermektedir. Ayrıca, belirli bir seviyeye kadar kuraklık stresi altında Asclepias curassavica tohumlarının çimlenmesinin korunduğu ve strese maruz kalmayan bitkilere göre kök ve sürgün büyümesinin arttığı sonucuna ulaşılmıştır. Bu bulgular, bu türün stres koşullarında adaptasyon yeteneğini vurgulamaktadır.

Anahtar Kelimeler

Çimlenme , Asclepias curassavica , Tuzluluk , Kuraklık stresi , Radikula , Plumula

Etik Beyan

There is no need to obtain permission from the ethics committee for this study.

Kaynakça

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