The Effect of Humic Acid Use on Reducing Salt Stress in Purple Basil (Ocimum basilicum L.)

Öz

Salt stress is a significant environmental stressor limiting plant development, yield potential and overall plant performance worldwide. Humic acid, a biostimulant agent, encourages plant growth and boosts stress tolerance to against the adverse impacts of salt stress. The current research intends to examine the activity of humic acid application in alleviating the detrimental influence of saltinity in purple basil (Ocimum basilicum L. cv. midnight). To achieve this goal, a completely randomised factorial experiment with three replications was designed. Two different humic acid doses (0 and 1000 mg L-1) and three varying salt treatments (0, 75 and 150 mM NaCl) were administered to the plants. Purple basil plants growth traits (plant height, leaf length, leaf fresh and dry weights), chlorophyll fluorescence (Fv/Fm), electrolyte leakage and photosynthetic pigment (chlorophyll a, chlorophyll b, total chlorophyll and carotenoids) levels were identified. Plant height, leaf length, fresh leaf weight, dry leaf weight, chlorophyll fluorescence (Fv/Fm), chlorophyll a and total chlorophyll level declined in salt stressed plants subjected in relative to the control group. Humic acid treatment helped with the elimination of the harmful influence of salinity on these parameters (except leaf length, chlorophyll a and total chlorophyll at 150 mM NaCl concentration). However, chlorophyll b and total carotenoid content in salt-treated plants showed no significant difference at 75 mM NaCl, whereas they decreased considerably at 150 mM NaCl. While the influence of humic acid treatment on chlorophyll b and total carotenoid content was statistically insignificant (p>0.05) at 75 mM NaCl, it was found to have decreasing effect at 150 mM NaCl. Furthermore, electrolyte leakage increased linearly with increasing salinity, while humic acid application only showed an ameliorating effect at 75 mM NaCl concentration. The results of this study showed that humic acid was successful in removing the impact of salt stress in purple basil at 75 mM NaCl.

Anahtar Kelimeler

• Abiotic stress
• Humic acid
• Photosynthetic pigments
• Purple basil
• Salinity

Mor Fesleğende (Ocimum basilicum L.) Tuz Stresinin Azaltılmasında Hümik Asit Kullanımının Etkisi

Öz

Tuzluluk stresi, dünya genelinde bitki büyümesini ve verimliliğini sınırlayan önemli bir çevresel stres faktörüdür. Hümik asit, bir biyostimülan olarak, bitkilerin gelişimini teşvik etmekte ve tuz stresinin zararlı etkilerine karşı bitki direncini artırmaktadır. Bu çalışma, mor fesleğende (Ocimum basilicum L. cv. midnight) tuz stresinin olumsuz etkilerini hafifletmede humik asit uygulamasının rolünü değerlendirmeyi amaçlamaktadır. Bu amaca ulaşmak için, üç tekrarlı tamamen rastgele faktöriyel bir deney tasarlanmıştır. Bitkilere iki farklı humik asit konsantrasyonu (0 ve 1000 mg L-1) ve üç farklı tuz seviyesi (0, 75 ve 150 mM NaCl) uygulanmıştır. Mor fesleğen bitkisinin büyüme parametreleri (bitki boyu, yaprak uzunluğu, yaprak taze ve kuru ağırlıkları), elektrolit sızıntısı, klorofil floresansı (Fv/Fm) ve fotosentetik pigment (klorofil a, b, toplam klorofil ve karotenoidler) seviyeleri belirlenmiştir. Bitki boyu, yaprak uzunluğu, yaprak taze ağırlığı, yaprak kuru ağırlığı, klorofil floresansı (Fv/Fm), klorofil a ve toplam klorofil içeriği tuz stresi altındaki bitkilerde kontrole kıyasla azalmıştır. Hümik asit uygulaması, tuzluluğun bu parametreler üzerindeki olumsuz etkilerini hafifletmeye yardımcı olmuştur (150 mM NaCl konsantrasyonunda yaprak uzunluğu, klorofil a ve toplam klorofil hariç). Bununla birlikte, tuz stresi altındaki bitkilerde klorofil b ve toplam karotenoid içeriği 75 mM NaCl'de önemli bir farklılık göstermezken, 150 mM NaCl'de belirgin şekilde azalmıştır. Hümik asit uygulamasının klorofil b ve toplam karotenoid içeriği üzerindeki etkisi 75 mM NaCl'de istatistiksel olarak önemsizken (p<0.05), 150 mM NaCl'de azaltıcı etkisi bulunmuştur. Ayrıca, elektrolit sızıntısı artan tuzlulukla doğrusal olarak artarken, hümik asit uygulaması sadece 75 mM NaCl konsantrasyonunda iyileştirici etki göstermiştir. Bu çalışmadan elde edilen sonuçlar, humik asidin mor fesleğende tuz stresinin neden olduğu hasarı hafifletmede 75 mM NaCl konsantrasyonunda başarılı sonuçlar verdiğini ortaya koymuştur.

Anahtar Kelimeler

• Abiyotik stres
• Hümik asit
• Fotosentetik pigmentler
• Mor fesleğen
• Tuzluluk

Kaynakça

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